| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,268,200 | 0 | T | A | 53.8% | ‑0.3 / 15.7 | 13 | I23F (ATT→TTT) | garP | galactarate/glucarate/glycerate transporter GarP |
| Reads supporting (aligned to +/- strand): ref base T (1/5); new base A (7/0); total (8/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.66e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.56e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CTGCAACTCTTTTGCCACTTCGGTACCAGCAATAGACAGCGTTGCACGATCGGCGTAGTTAACGGCGGTAACAATAAAAATAATCAGTAATATTAAATAGCGGGTATGCACGCCTTTCTTTTTTTCGTCAACGGTGTCCAGAATCATTTTATTTACCT > NZ_CP009273/3268120‑3268277 | cTGCAACTCTTTTGCCACTTCGGTACCAGCAATAGACAGCGTTGCACGATCGGCGTAGTTAACGGCGGTAACAATAAAAATAATCAGTaa < 1:198967/90‑1 (MQ=255) gCAACTCTTTTGCCACTTCGGTACCAGCAATAGACAGCGTTGCACGATCGGCGTAGTTAACGGCGGTAAAAAAAAAAAAAATCAGTAata > 1:569962/1‑90 (MQ=255) tttGCCACTTCGGTACCAGCAATAGACAGCGTTGCACGATCGGCGTAGTTAACGGCGGGAAAAAAAAAAAAAAACAGTAAAATTAAATaa > 2:709923/1‑89 (MQ=255) ttGCCACTTCGGTACCAGCAATAGACAGCGTTGCACGATCGGCGTAGTTAACGGCGGTAACAATAAAAATAATCAGTAATATTAAATAGc < 1:709923/90‑1 (MQ=255) aGCAATAGACAGCGTTGTACGATCGGCGTAGTTAACGGCGGTAACAATAAAAAAAATAAGAAAAATTAAAAAGCGGGTATGCACCCcttt > 1:596966/1‑90 (MQ=255) aGCAATAGACAGCGTTGCACGATCGGCGTAGTTAACGGCGGTAAAAATAAAAAAAAACAGTAATATTAAAAAACGGGGATGGACGCcttt > 2:158540/1‑90 (MQ=255) tAGACAGCGTTGCACGATCGGCGTAGTTAACGGCGGGAAAAAAAAAAAAAAAAAGTAATATTAAAAAGAGGGGATGCACGCCCTtttttt > 2:681742/1‑90 (MQ=255) gCGTTGCACGATCGGCGTAGTTAACGGCGGTAAAAATAAAAAAAATAAGGTATATTAAAAAGGGGGGGGTCACCCCCTTCTTTTTTTctg > 2:375142/1‑88 (MQ=255) gCGTTGCACGATCGGCGTAGTTAACGGCGGGAACAAAAAAAAAAATCAGTAATATTTAATAGCGGGGGTGGACGCCTttttttttttttt > 2:401642/1‑87 (MQ=255) cACGATCGGCGTAGTTAACGGCGGTAACAATAAAAATAATCAGTAATATTAAATAGCGGGTATGCACGCCTTTCTTTTTTTCGTCGACgg < 1:456359/90‑1 (MQ=255) gTTAACGGCGGTAACAATAAAAATAATCAGTAatat > 1:792816/1‑36 (MQ=255) gTTAACGGCGGTAACAATAAAAATAATCAGTAatat < 2:792816/36‑1 (MQ=255) tAACAATAAAAATAATCAGTAATATTAAATAGCGGGTATGCACGCCTTTCTTTTTTTCGTCAACGGTGTCCAGAATCATTTTATTTACCt < 1:401642/90‑1 (MQ=255) | CTGCAACTCTTTTGCCACTTCGGTACCAGCAATAGACAGCGTTGCACGATCGGCGTAGTTAACGGCGGTAACAATAAAAATAATCAGTAATATTAAATAGCGGGTATGCACGCCTTTCTTTTTTTCGTCAACGGTGTCCAGAATCATTTTATTTACCT > NZ_CP009273/3268120‑3268277 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |