| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,149,203 | 0 | A | C | 53.8% | 6.9 / 14.8 | 13 | T154P (ACC→CCC) | mdtB | multidrug efflux RND transporter permease subunit MdtB |
| Reads supporting (aligned to +/- strand): ref base A (5/1); new base C (0/7); total (5/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.66e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.68e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
ACTTGTTGCC‑GAGCGATCTGCCTAACCCGCCGGTTTACAGCAAA‑GTGAACCCGGCAGATCCGCCGATCATGACGCTCGCCGTCACCTCAACCGCCATGCCGATGACGCAAGTGGAAGATATGGTGGAAACCCGCGTCGCGCAGAAAATCTCGCAGATTTCCGGCG > NZ_CP009273/2149120‑2149284 | aCTTTTTGCCGGAGCGTTCTCCCAACCCCGCCGTTTTACAGCAAA‑GTGAACCCGGCAGATCCGCCGATCATGACGCTCGCCGTCACCTCa < 2:413248/90‑1 (MQ=255) gAGCGATCTGCCTAACCCGCCGGTTTACAGCAAA‑GTGAACCCGGCAGATCCGCCGATCATGACGCTCGCCGTCACCTCAACCGCCATGcc > 1:226402/1‑90 (MQ=255) caaccccGCCGTTTTAAAGAAAA‑GTGACCCCGGCAGACCCGCCGATCATGACGCTCGCCGTCCCCTAAACCGCCATGCCGATGACGCAAg < 2:859086/86‑1 (MQ=255) aCCCGCCGGTTTACAGCAAA‑GTGAACCCGGCAGATCCGCCGATCATGACGCTCGCCGTCACCTCAACCGCCATGCCGATGACGCAAGTgg > 1:1883204/1‑90 (MQ=255) cccGCCGGTTTACAGCAAA‑GTGAACCCGGCAGATCCGCCGATCATGACGCTCGCCGTCACCTCAACCGCCATGCCGATGACGCAAGTGGa > 2:1311275/1‑90 (MQ=255) gttttACAAAAAA‑GTAACCCCGGCAGACCCGCCGATCATGACGCTCGCCGTCCCCTCAACCGCCATGCCGATGACGCAAGTGGAAGatat < 2:193188/88‑1 (MQ=255) ggTTAACAGAAAA‑GTAACCCCGCCAGCCCCGCCGATCATGCCGCTCGCCGCCCCCTCAACCGCCATGCCGATGACGCAAGTGGAAGatat < 2:1587705/90‑1 (MQ=255) gTTTTCAACAAAGGTGACCCCGGCGGACCCGCCGATAATGACGCCCGCGGCCCCCTCAACCGCCATGCCGATGACGCAAGTGGAAGatat < 1:915804/90‑1 (MQ=255) cccGGCAGACCCGCCGATCATGACGCCCGCCGTCCCCTCAACCGCCATGCCGATGACGCAAGTGGAAGATATGGTGGAAACCCGCGTcgc < 2:416136/90‑1 (MQ=255) cgccgATCATGACGCTCGCCGCCCCCTCAACCGCCATGCCGATGACGCAAGTGGAAGATATGGTGGAAACCCGCGTCGCGCAGAAAAtct < 2:788190/90‑1 (MQ=255) tGACGCTCGCCGTCACCTCAACCGCCATGCCGATGACGCAAGTGGAAGATATGGTGGAAACCCGCGTCGCGCAGAAAATCTCGCAGAttt > 1:569620/1‑90 (MQ=255) tCGCCGTCACCTCAACCGCCATGCCGATGACGCAAGTGGAAGATATGGTGGAAACCCGCGTCGCGCAGAAAATCTCGCAGATTTCCGGCg > 1:1489772/1‑90 (MQ=255) ccgccgcCCCCTCAACCGCCATGCCGATGACGCAAGTGGAAGATATGGTGGAAACCCGCGTCGCGCAGAAAATCTCGCAGATTTCCGGCg < 2:1489772/89‑1 (MQ=255) | ACTTGTTGCC‑GAGCGATCTGCCTAACCCGCCGGTTTACAGCAAA‑GTGAACCCGGCAGATCCGCCGATCATGACGCTCGCCGTCACCTCAACCGCCATGCCGATGACGCAAGTGGAAGATATGGTGGAAACCCGCGTCGCGCAGAAAATCTCGCAGATTTCCGGCG > NZ_CP009273/2149120‑2149284 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |