| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,380,331 | 0 | T | G | 53.1% | 29.1 / 34.2 | 33 | intergenic (+21/‑82) | yfbL/yfbM | M28 family metallopeptidase/YfbM family protein |
| Reads supporting (aligned to +/- strand): ref base T (6/9); new base G (17/0); total (23/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.78e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.22e-03 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAA > NZ_CP009273/2380251‑2380406 | tATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGGTCCGaaaaa > 1:1307728/1‑90 (MQ=255) aTGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGc < 1:1500079/90‑1 (MQ=255) cTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACat < 2:588858/90‑1 (MQ=255) tCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACatt < 1:310/90‑1 (MQ=255) gTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGGCCCGAAAAAAAGACAAGGACaatttttttt > 1:656825/1‑90 (MQ=255) ggTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCCGAAAAAAAGACATGCACATTTTTTTTTTCCTCa > 2:761076/1‑90 (MQ=255) ggTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGGCCCGAAAAAAAGACAAGCACATTTTTTTTTTCCTcc > 2:1259535/1‑89 (MQ=255) tgtTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATc < 1:761076/90‑1 (MQ=255) gtTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGGTCCGAAAAAAAGAAAAGAACAATTTTTTTTTTCTCctct > 1:1435117/1‑90 (MQ=255) ataACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGGCGAGAAAAAAAAACAAACGCATATTATTTTTTCTCCTCTCtt > 1:1050774/1‑90 (MQ=255) cTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTaa < 1:812075/90‑1 (MQ=255) tttGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAAAGACATGCACATTTTTTTTTTCCTCATCTGtttttaa > 2:87492/1‑87 (MQ=255) tttGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAAAGACAAGCACATTTTTTTTTTTCTCCTCTTTTTTTAg > 2:1562344/1‑90 (MQ=255) tttGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGGCCCGAAAAAAAGACATGCACATTTTTTTTTTCCTCCTCTTTTTTTAg > 2:1782133/1‑90 (MQ=255) tttGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGGCCCGAAAAAAAGACAAGCACATTTTTTTTTTTCTCCTCTGtttttaa > 2:504649/1‑87 (MQ=255) tttGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGGCCAGAAAAAACGACATGCACATTTTTTTTTTCCTCATCTTTTTTTAg > 2:34125/1‑90 (MQ=255) tttGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGGCCAGAAAAAACGACAAGCACATTTTTTTTTTCCTCATCTGTTTTTAg > 2:137340/1‑90 (MQ=255) tttGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGGCCAGAAAAAAAGACAAGCACATTTTTTTTTTCCTCCTCTGtttttaa > 2:812075/1‑87 (MQ=255) acaGTAAATAATAAAAATTTCTCGAGGCGGGGCCCGAAAAAACGAAAAGCACAATTTTTTTTTCCCCCTCTGTTTTTAAATGCGaaaaaa > 2:1001584/1‑87 (MQ=255) acaGTAAATAATAAAAATTTCTCGAGGCGGGGCCAGAAAAAAAAAAAAGCACAATTTTTTTTTTCCCCTCTGGTTTTTAATGCGaaaaaa > 1:1535233/1‑87 (MQ=255) aaaTAATAAAAATTTCTCGAGGCGGGTGCCGAAAAAAAGACACGAACATTTTTTTTTTCCCCCTCTTTTTTTAGATGTGGAACATaaaaa > 1:594765/1‑89 (MQ=255) aaaTAATAAAAATTTCTCGAGGCGGGGGCAGAAAAAAAGACAAGAAAAATTTTTTTTTTCTCATTTGTTTTTAGATTGGAAAAAAGAAAt > 1:1190455/1‑90 (MQ=255) aaaTAATAAAAATTTCTCGAGGCGGGGCCAGAAAAAAAGACAAGCACATTTTTTTTTTCCTCCTCTTTTTTTAGATGGGGAAAAAGaaaa > 1:26410/1‑89 (MQ=255) aaaTAATAAAAATTTCTCGAGGCGGGGCCAGAAAAAAAGAAAAGCACAATTTTTTTTTTCTCCTCTGTTTTTAAATGTGAAAAAAGaaaa > 1:1819972/1‑89 (MQ=255) taataaAAATTTCTCGAGGCGGGGCCCGAAAAAAAGAAAAGGACAATTTTTTTTTCCTCCTCTGTTTTTAGATGGGAAAAAAGaaaatta > 1:1314982/1‑86 (MQ=255) ataaAAATTTCTCGAGGCGGGTCCAGAAAAAACGACATGCACATTTTTTTTTTCCTCATCTGTTTTTAGATGCGGAAAATGAAATTTaaa > 2:716829/1‑89 (MQ=255) ataaAAATTTCTCGAGGCGGGTCCAGAAAAAAAGACATGCACATTTTTTTTTTCCTCATCTGTTTTTAGATGCGAAAAATGAAAATTAAc > 2:225018/1‑90 (MQ=255) aaaaaTTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACgg < 1:156092/90‑1 (MQ=255) aaTTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACggagg < 2:160535/90‑1 (MQ=255) aTTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACggagga < 1:1562344/90‑1 (MQ=255) aTTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACggagga < 1:1860485/90‑1 (MQ=255) aTTTCTCGAGGCGGGCCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACggagga < 2:748078/90‑1 (MQ=255) tttCTCGAGGCGGGGCCAGAAAAAACGACAAGCACAATTTATTTTTTCTCCTCTGTTTTTAAATTGGAAAAAAAAAAATTAACAGAGGaa > 1:978417/1‑90 (MQ=255) | TATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAA > NZ_CP009273/2380251‑2380406 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |