Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092732,380,3570AT61.3% 4.3 / 33.1 31intergenic (+47/‑56)yfbL/yfbMM28 family metallopeptidase/YfbM family protein
Reads supporting (aligned to +/- strand):  ref base A (4/8);  new base T (19/0);  total (23/8)
Fisher's exact test for biased strand distribution p-value = 6.27e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.66e-05
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

GTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCTATTTTGCAGAAATTGACT  >  NZ_CP009273/2380272‑2380446
                                                                                     |                                                                                         
gTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGGCCCGAAAAAAAGACAAGGACaatttttttt                                                                                       >  1:656825/1‑90 (MQ=255)
      ggTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCCGAAAAAAAGACATGCACATTTTTTTTTTCCTCa                                                                                 >  2:761076/1‑90 (MQ=255)
      ggTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGGCCCGAAAAAAAGACAAGCACATTTTTTTTTTCCTcc                                                                                 >  2:1259535/1‑89 (MQ=255)
        tgtTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATc                                                                               <  1:761076/90‑1 (MQ=255)
         gtTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGGTCCGAAAAAAAGAAAAGAACAATTTTTTTTTTCTCctct                                                                              >  1:1435117/1‑90 (MQ=255)
            ataACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGGCGAGAAAAAAAAACAAACGCATATTATTTTTTCTCCTCTCtt                                                                           >  1:1050774/1‑90 (MQ=255)
                cTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTaa                                                                       <  1:812075/90‑1 (MQ=255)
                 tttGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAAAGACATGCACATTTTTTTTTTCCTCATCTGtttttaa                                                                      >  2:87492/1‑87 (MQ=255)
                 tttGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAAAGACAAGCACATTTTTTTTTTTCTCCTCTTTTTTTAg                                                                      >  2:1562344/1‑90 (MQ=255)
                 tttGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGGCCCGAAAAAAAGACATGCACATTTTTTTTTTCCTCCTCTTTTTTTAg                                                                      >  2:1782133/1‑90 (MQ=255)
                 tttGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGGCCCGAAAAAAAGACAAGCACATTTTTTTTTTTCTCCTCTGtttttaa                                                                      >  2:504649/1‑87 (MQ=255)
                 tttGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGGCCAGAAAAAACGACATGCACATTTTTTTTTTCCTCATCTTTTTTTAg                                                                      >  2:34125/1‑90 (MQ=255)
                 tttGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGGCCAGAAAAAACGACAAGCACATTTTTTTTTTCCTCATCTGTTTTTAg                                                                      >  2:137340/1‑90 (MQ=255)
                 tttGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGGCCAGAAAAAAAGACAAGCACATTTTTTTTTTCCTCCTCTGtttttaa                                                                      >  2:812075/1‑87 (MQ=255)
                            acaGTAAATAATAAAAATTTCTCGAGGCGGGGCCCGAAAAAACGAAAAGCACAATTTTTTTTTCCCCCTCTGTTTTTAAATGCGaaaaaa                                                           >  2:1001584/1‑87 (MQ=255)
                            acaGTAAATAATAAAAATTTCTCGAGGCGGGGCCAGAAAAAAAAAAAAGCACAATTTTTTTTTTCCCCTCTGGTTTTTAATGCGaaaaaa                                                           >  1:1535233/1‑87 (MQ=255)
                                 aaaTAATAAAAATTTCTCGAGGCGGGTGCCGAAAAAAAGACACGAACATTTTTTTTTTCCCCCTCTTTTTTTAGATGTGGAACATaaaaa                                                      >  1:594765/1‑89 (MQ=255)
                                 aaaTAATAAAAATTTCTCGAGGCGGGGGCAGAAAAAAAGACAAGAAAAATTTTTTTTTTCTCATTTGTTTTTAGATTGGAAAAAAGAAAt                                                      >  1:1190455/1‑90 (MQ=255)
                                 aaaTAATAAAAATTTCTCGAGGCGGGGCCAGAAAAAAAGACAAGCACATTTTTTTTTTCCTCCTCTTTTTTTAGATGGGGAAAAAGaaaa                                                      >  1:26410/1‑89 (MQ=255)
                                 aaaTAATAAAAATTTCTCGAGGCGGGGCCAGAAAAAAAGAAAAGCACAATTTTTTTTTTCTCCTCTGTTTTTAAATGTGAAAAAAGaaaa                                                      >  1:1819972/1‑89 (MQ=255)
                                    taataaAAATTTCTCGAGGCGGGGCCCGAAAAAAAGAAAAGGACAATTTTTTTTTCCTCCTCTGTTTTTAGATGGGAAAAAAGaaaatta                                                   >  1:1314982/1‑86 (MQ=255)
                                      ataaAAATTTCTCGAGGCGGGTCCAGAAAAAACGACATGCACATTTTTTTTTTCCTCATCTGTTTTTAGATGCGGAAAATGAAATTTaaa                                                 >  2:716829/1‑89 (MQ=255)
                                      ataaAAATTTCTCGAGGCGGGTCCAGAAAAAAAGACATGCACATTTTTTTTTTCCTCATCTGTTTTTAGATGCGAAAAATGAAAATTAAc                                                 >  2:225018/1‑90 (MQ=255)
                                        aaaaaTTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACgg                                               <  1:156092/90‑1 (MQ=255)
                                           aaTTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACggagg                                            <  2:160535/90‑1 (MQ=255)
                                            aTTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACggagga                                           <  1:1562344/90‑1 (MQ=255)
                                            aTTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACggagga                                           <  1:1860485/90‑1 (MQ=255)
                                            aTTTCTCGAGGCGGGCCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACggagga                                           <  2:748078/90‑1 (MQ=255)
                                             tttCTCGAGGCGGGGCCAGAAAAAACGACAAGCACAATTTATTTTTTCTCCTCTGTTTTTAAATTGGAAAAAAAAAAATTAACAGAGGaa                                          >  1:978417/1‑90 (MQ=255)
                                                                   aaGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCt                    >  1:1635119/1‑90 (MQ=255)
                                                                    aGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCTa                   >  1:501581/1‑90 (MQ=255)
                                                                          aTGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCTATTTTGc             <  2:699008/90‑1 (MQ=255)
                                                                                     attttCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCTATTTTGCAGAAATTGACt  <  1:1259535/90‑1 (MQ=255)
                                                                                     |                                                                                         
GTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCTATTTTGCAGAAATTGACT  >  NZ_CP009273/2380272‑2380446

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑