Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP009273349,9990TG52.9% 125.7 / 200.7 121V528G (GTG→GGG) prpEpropionate‑‑CoA ligase
Reads supporting (aligned to +/- strand):  ref base T (1/56);  new base G (63/1);  total (64/57)
Fisher's exact test for biased strand distribution p-value = 2.32e-32
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.84e-23
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 121 total aligned reads displayed.

TTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCG‑TTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCG  >  NZ_CP009273/349915‑350087
                                                                                    |                                                                                         
ttGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGGTGGGGGGaaaa                                                                                      >  2:25747/1‑89 (MQ=255)
ttGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGGGGCGGGGGGTGGGGGGGAAg                                                                                      >  1:1074515/1‑90 (MQ=255)
 tGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATTCGGGCGTTGCCGAAGAGGCGGTGGGTGGGGGGGAAGa                                                                                     >  2:680462/1‑90 (MQ=255)
    cGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATgc                                                                                  <  2:1610920/90‑1 (MQ=255)
          tCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGaa                                                                            <  2:167526/90‑1 (MQ=255)
          tCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGaa                                                                            <  2:1233890/90‑1 (MQ=255)
                   gACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAggt                                                                   <  2:1271777/90‑1 (MQ=255)
                   gACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAggt                                                                   <  2:1061912/90‑1 (MQ=255)
                      gcgcgAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGGGGAAGATGCGCTGAAAGGGCAgggggg                                                                >  2:1052780/1‑89 (MQ=255)
                      gcgcgAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGGGAAAGATGCGCTGAAAGGGCAgggggg                                                                >  2:1169663/1‑89 (MQ=255)
                      gcgcgAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGGGAAAGAAGCGCTGAAAAGGGAgggggg                                                                >  2:1887213/1‑89 (MQ=255)
                      gcgcgAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGATGCGCTGTAAGGGGAggtggc                                                                >  2:808639/1‑90 (MQ=255)
                      gcgcgAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGAAGGGCTGAAAGGGCAggtggg                                                                >  2:1303644/1‑89 (MQ=255)
                      gcgcgAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGTTGGGGGGAAAGATGCGCTGAAAGGGGAggtggc                                                                >  2:1216361/1‑90 (MQ=255)
                      gcgcgAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGTTGGGGGGAAAGAAGCGCTGAAAGGGCAgggggg                                                                >  2:470065/1‑89 (MQ=255)
                      gcgcgAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGGTGGGGGGAAAGATGCGCTGAAAGGGGAgggggg                                                                >  2:45488/1‑89 (MQ=255)
                      gcgcgAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGGTGGGGGGAAAGATGCGCTGAAAGGGCAggtggg                                                                >  1:1768078/1‑89 (MQ=255)
                       cgcgAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGGTGGGGGGGAAGATGCGCTGAAAGGGGAggtgggg                                                               >  2:688876/1‑88 (MQ=255)
                          gagaTTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGGTGGGGGGGAAGAAGAGCCGGAAGGGGAgggggggggg                                                            >  1:1534431/1‑88 (MQ=255)
                           agaTTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAggtggcggtgg                                                           <  2:1267150/90‑1 (MQ=255)
                            gaTTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGGGGAAAATGCGCTGAAAGGGGAgggggcgggggg                                                          >  2:577987/1‑89 (MQ=255)
                                 aaGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGAAGCGCCGAAAAGGCAGGGGGGGGGGGGG‑GTTg                                                    >  1:1572273/1‑90 (MQ=255)
                                   gagagTATCTCCAGCCATCCGGGTGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCG‑TTTGTc                                                  <  1:1372862/90‑1 (MQ=255)
                                   gagagTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCG‑TTTGTc                                                  <  2:806894/90‑1 (MQ=255)
                                   gagagTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCG‑TTTGTc                                                  <  1:1052780/90‑1 (MQ=255)
                                   gagagTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCG‑TTTGTc                                                  <  1:572741/90‑1 (MQ=255)
                                   gagagTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCG‑TTTGTc                                                  <  2:328623/90‑1 (MQ=255)
                                     gagTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCG‑TTTGTCAt                                                <  2:168/90‑1 (MQ=255)
                                     gagTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCG‑TTTGTCAt                                                <  2:1795574/90‑1 (MQ=255)
                                     gagTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGGGGAAGATGGGCTGAAAGGGCAGGTGGGGGGGGGG‑GTTGTCAt                                                >  2:411702/1‑90 (MQ=255)
                                     gagTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGGGAAAGATGCGCTGAAAGGGGAGGGGGGGGTGGCG‑GTTGTCAt                                                >  2:472573/1‑90 (MQ=255)
                                     gagTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGGGAAAGATGCGCTGAAAGGGCAGGGGGCGGGGGCG‑TTTGTCAt                                                >  2:309798/1‑90 (MQ=255)
                                     gagTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGGGAAAGAAGCGCTGAAAGGGCAGGGGGGGGGGGCG‑GTTGTCAt                                                >  2:1422271/1‑90 (MQ=255)
                                     gagTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGAAGCGCCGAAAGGGCAGGGGGGGGGGGGG‑GTTGTCAt                                                >  1:1819565/1‑90 (MQ=255)
                                     gagTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAAAAGCGCTGAAAGGGCAGGGGGGGGGGGGG‑GTTGTCAt                                                >  2:1772613/1‑90 (MQ=255)
                                     gagTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAGATGCGCTGAAAGGGGAGGGGGGGGGGGGG‑GTTGTCAt                                                >  1:85023/1‑90 (MQ=255)
                                     gagTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGGTGGGGGGGAAGAAGCGCTGAAAGGGGAGGGGGGGGGGGGG‑GTTTTCAt                                                >  1:781617/1‑90 (MQ=255)
                                     gagTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGGTGGGGGGAAAGATGGGCTGAAAGGGGAGGGGGCGGGGGGG‑GTTGTCAt                                                >  1:66553/1‑90 (MQ=255)
                                     gagTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGGTGGGGGGAAAGATGCGCTGAAAGGGGAGGGGGGGGGGGGG‑GTTGTTAt                                                >  1:248917/1‑90 (MQ=255)
                                     gagTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGGTGGGGGGAAAAATGCGCTGAAAGGGGAGGGGGGGGGGGGG‑GTTGTCAt                                                >  2:1045256/1‑90 (MQ=255)
                                     gagTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGGTGGGGGGAAAAATGCGCTGAAAGGGGAGGGGGCGGGGGGG‑GTTGTCAt                                                >  1:671138/1‑90 (MQ=255)
                                     gagTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGGGGGGGGGGAAGATGCGGTGGAAGGGGGGGGGGGGGTGGCG‑GGTGTCag                                                >  1:1196274/1‑89 (MQ=255)
                                      agTATCTCCAGCCATCC‑GGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCG‑TTTGTCATTc                                              <  2:1547668/90‑1 (MQ=255)
                                       gTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGGGAAAGATGCGCTGAAAGGGCAGGGGGCGGGGGCG‑TTTGTCATTc                                              >  2:464070/1‑90 (MQ=255)
                                       gTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAAATGCGCTGAAAGGGGAGGGGGGGGGGGGG‑GTTGTCATTc                                              >  1:168/1‑90 (MQ=255)
                                       gTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGTTGGGGGGAAAGATGGGCTGAAAAGGGAGGGGGGGGGGGGG‑GTTGTTAttt                                              >  2:1165413/1‑89 (MQ=255)
                                          tctcCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATgcg                                                                                 <  1:1827178/53‑1 (MQ=255)
                                          tctcCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGGGAAAGATgcg                                                                                 >  2:1827178/1‑53 (MQ=255)
                                          tctcCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAGATGCGCTGAAAGGGCAGGGGGCGGGGGCG‑GTTGTCATTTCGa                                           >  2:242179/1‑90 (MQ=255)
                                               aGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGAt                                                                                    >  2:1296848/1‑45 (MQ=255)
                                               aGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCG‑TTTGTCATTCCGAAagag                                      <  2:651357/90‑1 (MQ=255)
                                               aGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGATGCGCTGAAAGGGGAGGGGGCGGGGGGG‑GTTGTCATTCCGAAagag                                      >  2:1278536/1‑90 (MQ=255)
                                               aGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAAATGCGCTGGAAGGGGAGGGGGGGGGGGGG‑TTTGGCATTTCCGAagag                                      >  1:778916/1‑90 (MQ=255)
                                               aGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAAATGCGCTGAAAGGGGAGGGGGGGGGGGGG‑GTTGTCATTTCGAAaaag                                      >  2:1897712/1‑90 (MQ=255)
                                               aGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAAATGCGCTGAAAGGGGAGGGGGGGGGGGGG‑GTTGTTATTCCGAAaaag                                      >  1:651357/1‑90 (MQ=255)
                                               aGCCATCCGGGCGTTGCCGAAGTGGCGGGGGTTGGGGGGAAAGATGCGCTGAAAGGGGAGGGGGGGGGGGCG‑TTTGTCATTCCGAAagag                                      >  2:575763/1‑90 (MQ=255)
                                               aGCCATCCGGGCGTTGCCGAAGTGGCGGGGGGTGGGGGGAAAAAAGCGCCGAAAGGGGAGGGGGGGGGGGGG‑GGTGTGATTCCGGAagag                                      >  1:1231670/1‑90 (MQ=255)
                                                 ccATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAGATGCGCTGAAAGGGGAGGGGGGGGGGGGG‑GTTGTCATTTCGAAagagag                                    >  2:1555410/1‑90 (MQ=255)
                                                  cATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCG‑TTTGTCATTCCGAAAGAGAGc                                   <  2:1420293/90‑1 (MQ=255)
                                                   aTCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGGGAAAGAAGCGCTGAAAAGGGAGGTGGGGGGGGGG‑GTTGTCATTCCCAAaaagaggg                                  >  2:1572130/1‑88 (MQ=255)
                                                   aTCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGATGCGCTGGAAGGGCAGGGGGGGGGGGGG‑GTTGTTATTCCGAAAGAGAGCg                                  >  2:1621461/1‑90 (MQ=255)
                                                   aTCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAGATGCGCTGAAAGGGGAGGGGGGGGGGGGG‑GTTGTTATTTCGGAAGAGAGCg                                  >  1:350747/1‑90 (MQ=255)
                                                    tCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCG‑TTTGTCATTCCGAAAGAGAGCGa                                 <  1:472573/90‑1 (MQ=255)
                                                     ccGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCG‑TTTGTCATTCCGAAAGAGAGCGAc                                <  1:411702/90‑1 (MQ=255)
                                                     ccGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCG‑TTTGTCATTCCGAAAGAGAGCGAc                                <  1:925775/90‑1 (MQ=255)
                                                     ccGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCG‑TTTGTCATTCCGAAAGAGAGCGAc                                <  2:183450/90‑1 (MQ=255)
                                                     ccGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCG‑TTTGTCATTCCGAAAGAGAGCGAc                                <  1:526761/90‑1 (MQ=255)
                                                     ccGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCG‑TTTGTCATTCCGAAAGAGAGCGAc                                <  1:1346600/90‑1 (MQ=255)
                                                     ccGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCG‑TTTGTCATTCCGAAAGAGAGCGAc                                <  1:1398642/90‑1 (MQ=255)
                                                     ccGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCG‑TTTGTCATTCCGAAAGAGAGCGAc                                <  1:1364542/90‑1 (MQ=255)
                                                     ccGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCG‑TTTGTCATTCCGAAAGAGAGCGAc                                <  1:721072/90‑1 (MQ=255)
                                                     ccGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCG‑TTTGTCATTCCGAAAGAGAGCGAc                                <  1:317057/90‑1 (MQ=255)
                                                     ccGGGCGTTGCCGAAGTGGCGGTGGTTGGGGGGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCG‑TTTGTCATTCCGAAAGAGAGCGAc                                >  2:489753/1‑90 (MQ=255)
                                                       gggCGTTGCCGAGGTGGCGGGGGTTGGGGGGAAAGATTCGCCGAAAGGGCAGGTGGCGGTGGCG‑TTTGTCATTCCGAAAGAGAGCGACAg                              <  1:20131/90‑1 (MQ=255)
                                                       gggCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAAAAGCGCTGAAAGGGGGGGGGGGGGGGGGG‑GTTGTCATTTCGAAAAAGAGCGACAg                              >  1:1534731/1‑90 (MQ=255)
                                                       gggCGTTGCCGAAGTGGCGGGGGGTGGGGGGAAAGATGCGCTGAAAGGGCAGGGGGGGGGGGCG‑GTTGGCATTTCGGAAGAGAGCGGCAg                              >  1:115577/1‑90 (MQ=255)
                                                            ttGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCG‑TTTGTCATTCCGAAAGAGAGCGACAGTCTgg                         <  1:307556/90‑1 (MQ=255)
                                                            ttGCCGAAGTGGCGGGGGTTGGGGGGAAAGATGCGCTGAAAGGGGAGGGGGGGGGGGGG‑GTTGTCATTTCGAAAGAGAGCGGCAGGCTgg                         >  1:1521554/1‑90 (MQ=255)
                                                             tGCCGAAGTGGCGGTGGTTGGGGGGGAAGATGCGCTGAAAGGGGGGGGGGGGGGGGCG‑TTTGGGATTCCGAAAAAGAGGGCCAGTCTGGa                        >  2:1804057/1‑90 (MQ=255)
                                                              gCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCG‑TTTGTCATTCCGAAAGAGAGCGACAGTCTGGaa                       <  2:1492938/90‑1 (MQ=255)
                                                              gCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCG‑TTTGTCATTCCGAAAGAGAGCGACAGTCTGGaa                       <  1:670484/90‑1 (MQ=255)
                                                              gCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCG‑TTTGTCATTCCGAAAGAGAGCGACAGTCTGGaa                       <  2:1465499/90‑1 (MQ=255)
                                                               ccGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCG‑TTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAg                      <  1:1527589/90‑1 (MQ=255)
                                                               ccGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCG‑TTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAg                      <  1:1887213/90‑1 (MQ=255)
                                                                 gAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCG‑TTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGAt                    <  1:516504/90‑1 (MQ=255)
                                                                 gAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCG‑TTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGAt                    <  2:152924/90‑1 (MQ=255)
                                                                      ggCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCG‑TTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGa               <  2:204823/90‑1 (MQ=255)
                                                                      ggCGGTGGTTGGGGGGAAAGATGCGCTGAAAGGGCAGGGGGCGGGGGCG‑GTTTTCATTCCGAAAAAGAGCGACAGTCTGGGAGATCGTGa               >  2:907247/1‑90 (MQ=255)
                                                                      ggCGGTGGGTGGGGGGAAAGATGCGCTGAAAGGGCAGGGGGGGGGGGCG‑GTTTTTATTTCGAAAGAGAGGGACAGGCTGGGAGATCgggg               >  1:354294/1‑87 (MQ=255)
                                                                         ggtggtTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCG‑TTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGAtgt            <  1:45520/90‑1 (MQ=255)
                                                                         ggtggtTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCG‑TTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGAtgt            <  1:76790/90‑1 (MQ=255)
                                                                            gggtGGGGGGAAAGGGGCGCGGGAAGGGCAGGTGGCGGGGGCGTTTTTTCATT‑CAAAAAAGGGGGAAATTTTTGAAGATCGGGATGTggt         >  2:1241558/4‑89 (MQ=255)
                                                                               tGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCG‑TTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCa      <  1:131017/90‑1 (MQ=255)
                                                                               tGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCG‑TTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCa      <  1:18211/90‑1 (MQ=255)
                                                                                ggggTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCG‑TTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCAc     <  1:334702/90‑1 (MQ=255)
                                                                                ggggTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCG‑TTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCAc     <  2:1002321/90‑1 (MQ=255)
                                                                                ggggTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCG‑TTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCAc     <  2:1648063/90‑1 (MQ=255)
                                                                                ggggTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCG‑TTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCAc     <  2:925444/90‑1 (MQ=255)
                                                                                ggggTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCG‑TTTGTCATTCCGAAAGAGAGCAACAGTCTGGAAGATCGTGATGTGGCGCAc     <  2:1588036/90‑1 (MQ=255)
                                                                                   gTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCG‑TTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCg  <  2:332791/90‑1 (MQ=255)
                                                                                    |                                                                                         
TTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCG‑TTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCG  >  NZ_CP009273/349915‑350087

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑