| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 687,303 | 0 | A | G | 52.5% | 45.3 / 74.9 | 59 | intergenic (‑63/+27) | corC/ybeY | CNNM family magnesium/cobalt transport protein CorC/rRNA maturation RNase YbeY |
| Reads supporting (aligned to +/- strand): ref base A (8/20); new base G (31/0); total (39/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.11e-09 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.29e-08 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
ACTGTGTGAATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCATAATCTCTGT > NZ_CP009273/687217‑687386 | actGTGTGAATTGTCGTCGCTCATGGCGTCGGATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGGCGt > 2:1282803/1‑90 (MQ=255) tgtgtgAATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCt > 2:1051679/1‑90 (MQ=255) gtgtgAATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGGCGTCTg > 2:145768/1‑90 (MQ=255) gtgtgAATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGGCGTCTg > 2:388995/1‑90 (MQ=255) gtgtgAATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGGCGTCTg > 2:29497/1‑90 (MQ=255) gtgtgAATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGGCGTCTg > 2:159135/1‑90 (MQ=255) gtgtgAATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGGCGGCTg > 2:863773/1‑90 (MQ=255) gtgtgAATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGGCGGCTg > 2:833957/1‑90 (MQ=255) gtgtgAATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGGCGGCTg > 1:1659946/1‑90 (MQ=255) gtgtgAATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCtt > 2:901027/1‑89 (MQ=255) gtgAATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCCCCAACGGCGGGGACGTCTGcc < 1:355019/90‑1 (MQ=255) gtgAATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGGCGTCTGcc > 2:1110363/1‑90 (MQ=255) gtgAATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGcc < 1:1145812/90‑1 (MQ=255) aaTTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGGCGTCTGCCAGt > 2:1404982/1‑90 (MQ=255) ttGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGGCGGCTGCCAGTCa > 2:1485635/1‑90 (MQ=255) tcgtcaCTCATGGCGTCGTATGGGTTCTCATGTTAGGTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGGCGCCGGCCAGTCaaag > 1:1258804/1‑89 (MQ=255) gtcgCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGGCGTCTGCCCGTTAAAAGc > 1:895305/1‑90 (MQ=255) cTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTgg < 2:934315/90‑1 (MQ=255) cTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTgg < 2:1051780/90‑1 (MQ=255) tCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGGCGGCTGCCAGGCAAATGCCTGGc > 2:1822663/1‑90 (MQ=255) tCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAAtggctggc > 2:352818/1‑90 (MQ=255) tcgtATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGGCGTCTGcc > 2:984875/1‑67 (MQ=255) tcgtATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGcc < 1:984875/67‑1 (MQ=255) cagtATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAattatt < 2:1705358/88‑1 (MQ=255) cgtATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGGCGGCTGCCCGTCAAAAACCTGGCAAattattt > 1:778887/1‑89 (MQ=255) ggTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGGCGGCTGCCCGTCAAAAACCTGGGAAATTATTTTTtttc > 1:1842473/1‑90 (MQ=255) ttCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGGCGTCTGCCAGTCAAAAGCCTGGCAAATTATTTTTTTTCgg > 2:675017/1‑90 (MQ=255) tctcATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGGCGTCTTCCCGTCAAAAGCCTGGCCAAATATTTTTTTTCCGc > 1:952092/1‑90 (MQ=255) cagcATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGc < 2:1315449/87‑1 (MQ=255) ctcATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGGCTGCCAGTCAAATGCCCGGCAAATTATTTTTTTTCGGCa > 2:1210909/1‑90 (MQ=255) tcATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGGGGGCTGCCAGTCAAAAACCGGGCCAAATATTTTTTTTCGGCaa > 1:57675/1‑90 (MQ=255) tcATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGGCGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTTTCGGCaa > 2:289947/1‑90 (MQ=255) cATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGGCGTCTGCCAGTCAAATGCCTGGCAAATTATTTTTTTTCGGCAAt > 2:124290/1‑90 (MQ=255) ttagttaATCAAAACGCCGTCGTTAATCACCAACGGCGGGGGCGGCTGCCAGTCAAAAGCCTGGCAAATTTTTTTTTTTCGGCAATGTAc > 1:261716/1‑90 (MQ=255) ttagttaATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTAc < 1:1051679/90‑1 (MQ=255) tagttaATCAAAACGCCGTCGTTAATCACCAACGGCGGGGGGGGCTGCCCGGCAAAAGCCCCGCAAAATATTCTTTCCCGGCAATGTACg > 1:1515987/1‑90 (MQ=255) agttaATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACgg < 2:1513220/90‑1 (MQ=255) gttaATCAAAACGCCGTCGTTAATCACCAACGGCGGGGGCGTCTGCCAGTCAAAAGCCTGGCAAATTATTTTTTTTCGGCAATGTAAGGa > 2:1285311/1‑90 (MQ=255) ttaATCAAAACGCCGTCGTTAATCACCAACGGCGGGGGCGGGTGCCAGTCCAATAACTTGCAAAATATTTCTTCTTCGCCAAGGACGGAt > 1:1226819/1‑90 (MQ=255) ttaATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGAt < 1:1548500/90‑1 (MQ=255) taATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATc < 1:836073/90‑1 (MQ=255) taATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATc < 1:933157/90‑1 (MQ=255) taATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATc < 2:284302/90‑1 (MQ=255) taATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATc < 1:291592/90‑1 (MQ=255) aaTCAAAACGCCGTCGTTAATCACCAACGGCGGGGGCGTCTGCCAGGCAAATGCCTGGCAAATTATTTTTTTTCGGCAATGTACGGGTcc > 2:1006584/1‑90 (MQ=255) aaTCAAAACGCCGTCGTTAATCACCAACGGCGGGGGCGGCTGCCCGTCAAAAGCCTGGCAAATTATTTTTTTTCGGCAATGTACGGGTcc > 1:1731805/1‑90 (MQ=255) aaTCAAAACGCCGTCGTTAATCACCAACGGCGGGGGCGGCTGCCCGTCAAAAGCCTGGCAAAATATTTTTTTTCGGCAATGTACgggctg > 1:1315449/1‑86 (MQ=255) aaTCAAAACGCCGTCGTTAATCACCAACGGCGGGGGCGGCTGCCAGTCAAAAGCCTGGCAAAATATTTTTTTTCGGCAAAGTACGGGTcc > 2:1772259/1‑90 (MQ=255) aaaaCGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCAt < 2:154514/90‑1 (MQ=255) gCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGccc < 1:1285311/90‑1 (MQ=255) gtcgtTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCaga < 1:1190448/90‑1 (MQ=255) gtcgtTAATCACCAACGGCGGGGACGGCTGCCCGGCAAAAGCCTGGCAAAATATTCTTTTTCGGCAAAGGACGGGTCCCCATAGCCCcga > 1:308881/1‑90 (MQ=255) tAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTTTCGGCAATGTACGGATCCCCATAACCCAGagcaaa > 2:1803494/1‑89 (MQ=255) caccacCGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCATa < 1:1515333/90‑1 (MQ=255) cACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCATa < 1:1772607/90‑1 (MQ=255) ccAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCATAAt > 2:695738/1‑90 (MQ=255) aaCGGCGGGGGCGTCTGCCAGTCAAAAGCCTGGCAAATTATTTTTTTTCGGCAATGTACGGATCCTCATAGCCCCGAGCAAACATAAtct > 1:592642/1‑90 (MQ=255) aaCGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCATAAtct < 2:653616/90‑1 (MQ=255) gcggGGACGTCTGCCCGTCAAAAGCCTGGCAAATTATTTTTTTTTGGCAAAGTACGGATCCTCATAGCCCACAGCAAGCATAATCTCTGt > 1:1664409/1‑90 (MQ=255) | ACTGTGTGAATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCATAATCTCTGT > NZ_CP009273/687217‑687386 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |