| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,213,870 | 0 | A | C | 60.7% | 24.3 / 28.7 | 28 | T363P (ACC→CCC) | pdeG | cyclic diguanylate phosphodiesterase |
| Reads supporting (aligned to +/- strand): ref base A (8/3); new base C (0/17); total (8/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.31e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.40e-05 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TCATATTGGAATTAATTTTAGCGCTTCGCATATTATTTCGCCGACGTTTGTCGACGAGTGTTTAAATTTCCGTGACAGTTTTACCCGCCGCGATTTAAACCTTGTTCTGGAAGTCACCGAGCGTGAGCCATTGAATGTTGATGAAAGTCTGGTTCAGCGGTTGAACAT > NZ_CP009273/1213788‑1213955 | tCATATTGAAATTATTTTTAGCGCTTCGAATATTTTTCCGCCGACGTTTGTCGACGAGTTTTTAAATTTCCGTGACATTTTTCCccgccg < 1:653477/90‑1 (MQ=255) cttatTGAAATTATTTTTAGCGCTTCGCATATTTTTCCGCCGACTTTTGTCGACGAGTTTTTAATTTTCCGTGACATTTTTCCCcgccgc < 1:636876/88‑1 (MQ=255) cATATTGGAATTATTTTTAGCGCTTCGCATTTTTTTCCGCCGCCTTTTGTCGACGAGTTTTAAATTTTCCGTGACATTTTTCCCcgccgc < 1:390049/90‑1 (MQ=255) tGGAATTAATTTTAGCGCTTCGCATATTATTTCGCCGACGTTTGTCGACGAGTGTTTAAATTTCCGTGACAGTTTTACCCGCCGCGAttt > 2:636876/1‑90 (MQ=255) tatttttAGCTCTTCGCATATTTTTCCGCCGCCTTTTGTCGACGATTTTTTAATTTCCCGTAACATTTTTCCCCGCCGCGATTTAAACCt < 1:1491548/87‑1 (MQ=255) taattTTAGCGCTTCGCATATTATTTCGCCGACGTTTGTCGACGAGTGTTTAAATTTCCGTGACAGTTTTACCCGCCGCGATTTAAACCt < 2:297488/90‑1 (MQ=255) gtttttAGCTCTTCGCATTTTTTTTCGCCGACTTTTGTCGACGAGTTTTTAAATTTCCGTGACATTTTTCCCCGCCGCGATTTAAACCtt < 1:262727/88‑1 (MQ=255) atttttAGCGCTTCGCATATTATTTCGCCGACGTTTGTCGACGAGTTTTTAAATTTCCGTGACAGTTTTCCCCGCCGCGATTTAAACCtt < 2:418138/88‑1 (MQ=255) cctttCGCATATTATTTCGCCGACTTTTGTCGACGAGTTTTTAAATTTCCGTGACATTTTTCCCCGCCGCGATTTAAACCTTGTTCTGGa < 2:1439359/87‑1 (MQ=255) ttCGCATATTATTTCGCCGACGTTTGTCGACGAGTGTTTAAATTTCCGTGACAGTTTTACCCGCCGCGATTTAAACCTTGTTCTGGAAgc > 2:262727/1‑89 (MQ=255) ttattTCGCCGACGTTTGTCGACGAGTGTTTAAATTTCCGTGACAGTTTTACCCGCCGCGATTTAAACCTTGTTCTGGAAGTCACCGAGc > 2:149341/1‑90 (MQ=255) tattTCGCCGACGTTTGTCGACGAGTGTTTAAATTTCCGTGACAGTTTTACCCGCCGCGATTTAAACCTTGTTCTGGAAGTCACCGAGCg > 1:869209/1‑90 (MQ=255) attTCGCCGACGTTTGTCGACGAGTGTTTAAATTTCCGTGACAGTTTTACCCGCCGCGATTTAAACCTTGTTCTGGAAGTCACCGAGCGt > 1:723499/1‑90 (MQ=255) tCGCCGCCTTTTGGCGACGATTTTTTAATTTCCCGTGACATTTTTCCCCGCCGCGATTTAAACCTTGTTCTGGAAGTCACCGAGCGTGAg < 1:1596721/90‑1 (MQ=255) tCGCCGACGTTTGTCGACGAGTGTTTAAATTTCCGTGACAGTTTTACCCGCCGCGATTTAAACCTTGTTCTGGAAGTCACCGAGCGTGAg > 1:482169/1‑90 (MQ=255) tCGCCGACGTTTGTCGACGAGTGTTTAAATTTCCGTGACAGTTTTACCCGCCGCGATTTAAACCTTGTTCTGGAAGTCACCGAGCGTGAg > 2:1598331/1‑90 (MQ=255) cGCCGACGTTTGTCGACGAGTTTTTAAATTTCCGTGACAGTTTTCCCCGCCGCGATTTAAACCTTGTTCTGGAAGTCACCGAGCGTGAGc < 2:497542/90‑1 (MQ=255) cGCCGACGTTTGTCGACGAGTGTTTAAATTTCCGTGACAGTTTTACCCGCCGCGATTTAAACCTTGTTCTGGAAGTCACCGAGCGTGAGc > 1:946916/1‑90 (MQ=255) gccttttGTCGAGGTGTTTTTAATTTTCCGTGACATTTTTCCCCGCCGCGATTTAAACCTTGTTCTGGAAGTCACCGAGCGTGAGCCAtt < 1:1764754/86‑1 (MQ=255) tCCGTGACGTTTTTCCCCGCCGCGATTTAAACCTTGTTCTGGAAGTCACCGAGCGTGAGCCATTGAATGTTGATGAAAGTCTGGTTCAGc < 2:1290022/90‑1 (MQ=255) tCCGTGACATTTTTCCCCGCCGCGATTTAAACCTTGTTCTGGAAGTCACCGAGCGTGAGCCATTGAATGTTGATGAAAGTCTGGTTCAGc < 2:1691873/90‑1 (MQ=255) tCCGTGACATTTTTCCCCGCCGCGATTTAAACCTTGTTCTGGAAGTCACCGAGCGTGAGCCATTGAATGTTGATGAAAGTCTGGTTCAGc < 1:306449/90‑1 (MQ=255) cGTGACGTTTTTCCCCGCCGCGATTTAAACCTTGTTCTGGAAGTCACCGAGCGTGAGCCATTGAATGTTGATGAAAGTCTGGTTCAGCgg < 1:337431/90‑1 (MQ=255) cGTGACGTTTTTCCCCGCCGCGATTTAAACCTTGTTCTGGAAGTCACCGAGCGTGAGCCATTGAATGTTGATGAAAGTCTGGTTCAGCgg < 1:209709/90‑1 (MQ=255) cGTGACAGTTTTACCCGCCGCGATTTAAACCTTGTTCTGGAAGTCACCGAGCGTGAGCCATTGAATGTTGATGAAAGTCTGGTTCAGCgg < 2:723499/90‑1 (MQ=255) tGACAGTTTTCCCCGCCGCGATTTAAACCTTGTTCTGGAAGTCACCGAGCGTGAGCCATTGAATGTTGATGAAAGTCTGGTTCAGCGGtt < 2:981980/90‑1 (MQ=255) cAGTTTTACCCGCCGCGATTTAAACCTTGTTCTGGAAGTCACCGAGCGTGAGCCATTGAATGTTGATGAAAGTCTGGTTCAGCGGTTGaa < 1:1598331/90‑1 (MQ=255) ttttCCCCGCCGCGATTTAAACCTTGTTCTGGAAGTCACCGAGCGTGAGCCATTGAATGTTGATGAAAGTCTGGTTCAGCGGTTGAACAt < 2:1153701/90‑1 (MQ=255) | TCATATTGGAATTAATTTTAGCGCTTCGCATATTATTTCGCCGACGTTTGTCGACGAGTGTTTAAATTTCCGTGACAGTTTTACCCGCCGCGATTTAAACCTTGTTCTGGAAGTCACCGAGCGTGAGCCATTGAATGTTGATGAAAGTCTGGTTCAGCGGTTGAACAT > NZ_CP009273/1213788‑1213955 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |