| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 642,203 | 0 | T | G | 65.0% | ‑1.5 / 16.5 | 20 | T255P (ACC→CCC) | citG | triphosphoribosyl‑dephospho‑CoA synthase CitG |
| Reads supporting (aligned to +/- strand): ref base T (1/6); new base G (13/0); total (14/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.81e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.63e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAAT‑GCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTT > NZ_CP009273/642117‑642287 | cAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTCg > 2:501780/1‑90 (MQ=255) aGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGtcgga > 1:255538/1‑86 (MQ=255) gCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTTGGAT‑Gcccccc > 2:637983/1‑89 (MQ=255) aCTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGGCGCAT‑Ggcccccct > 1:1250837/1‑87 (MQ=255) gccgccTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTGGGATGGCCCCCttttt > 1:516100/1‑90 (MQ=255) cTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAAT‑GCCCACTTTTTGCaata < 1:595281/90‑1 (MQ=255) gagaTTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTTGAAT‑GCCCCCCTTTTTCAATAATGtttt > 2:413416/1‑89 (MQ=255) gaTTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTTGAAT‑GCCCCCCTTTTTCAATAAAGTTTtcg > 2:605883/1‑90 (MQ=255) cgttcgATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAAT‑GCCCCCTTTTTGCAATAATGTTTGCGCCTcg < 2:524732/90‑1 (MQ=255) gATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAAT‑GCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTg < 1:1058047/90‑1 (MQ=255) cTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTGGGAT‑GCCCCCCTTTTTCAATAAATTTTTTGCCCCCCGCCGTAGccc > 2:1058047/1‑89 (MQ=255) cccTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTCGAAT‑GCCCCCCTTTTTTAATAATATTTTTGCCCCCCGCCGTTGCCCgc > 2:1352301/1‑90 (MQ=255) ccTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTCGAAT‑GCCCCCCTTTTTCCATAATATTTTCGCCCCCCGCCGTAGCCAgcg > 1:367158/1‑90 (MQ=255) tGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTGGAAT‑GCCCCCCTTTTTTAAAAAAATTTTCGCCCCCCCCCGTTGCCAGCGCa > 1:243519/1‑90 (MQ=255) tGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGGTGAAT‑GCCCCCCTTTTTCAAAAAAGTTTTTGCCTCGCGCCGTAGACAgcgac > 2:972762/1‑88 (MQ=255) cTGCCGGAGATAATCGAGATCGGCGGGGGTTTGGAT‑GCCCCCCTTTTTCGATAATTTTTTTGCCTCCCGCTTTTGGCCGCGCCGGgcccc > 1:213893/1‑90 (MQ=255) gCCGGAGATAATCGAGATCGGCGGGGGGTGGGAT‑GCCCCCCTTTTTTTATAATTTTTTTGCCCCCCGCCGTAGCCCGCGCCGGccccgtc > 1:545567/1‑87 (MQ=255) tCGAGATCGGCGGGGGTTCGAAT‑GCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACcgcgc < 2:347311/90‑1 (MQ=255) agaTCGGCGGGGGGTCGAAT‑GCCCCCCTTTTTTAATAATGTTTTCGCCTCCCGCTGTAGCCAGCGCCGGCCCCCCCCCCCCCCCCCCGAt > 2:1625643/1‑90 (MQ=255) aTCGGCGGGGGTTCGAAT‑GCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGc < 1:466187/90‑1 (MQ=255) gggggTTCGAAT‑GCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGtt < 1:247315/90‑1 (MQ=255) | CAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAAT‑GCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTT > NZ_CP009273/642117‑642287 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |