| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 642,216 | 0 | T | C | 55.6% | 3.9 / 21.4 | 18 | K250K (AAA→AAG) | citG | triphosphoribosyl‑dephospho‑CoA synthase CitG |
| Reads supporting (aligned to +/- strand): ref base T (1/7); new base C (10/0); total (11/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.51e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.38e-04 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAAT‑GCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTT > NZ_CP009273/642132‑642299 | gccgccTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTGGGATGGCCCCCttttt > 1:516100/1‑90 (MQ=255) cTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAAT‑GCCCACTTTTTGCaata < 1:595281/90‑1 (MQ=255) gagaTTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTTGAAT‑GCCCCCCTTTTTCAATAATGtttt > 2:413416/1‑89 (MQ=255) gaTTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTTGAAT‑GCCCCCCTTTTTCAATAAAGTTTtcg > 2:605883/1‑90 (MQ=255) cgttcgATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAAT‑GCCCCCTTTTTGCAATAATGTTTGCGCCTcg < 2:524732/90‑1 (MQ=255) gATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAAT‑GCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTg < 1:1058047/90‑1 (MQ=255) cTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTGGGAT‑GCCCCCCTTTTTCAATAAATTTTTTGCCCCCCGCCGTAGccc > 2:1058047/1‑89 (MQ=255) cccTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTCGAAT‑GCCCCCCTTTTTTAATAATATTTTTGCCCCCCGCCGTTGCCCgc > 2:1352301/1‑90 (MQ=255) ccTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTCGAAT‑GCCCCCCTTTTTCCATAATATTTTCGCCCCCCGCCGTAGCCAgcg > 1:367158/1‑90 (MQ=255) tGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTGGAAT‑GCCCCCCTTTTTTAAAAAAATTTTCGCCCCCCCCCGTTGCCAGCGCa > 1:243519/1‑90 (MQ=255) tGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGGTGAAT‑GCCCCCCTTTTTCAAAAAAGTTTTTGCCTCGCGCCGTAGACAgcgac > 2:972762/1‑88 (MQ=255) cTGCCGGAGATAATCGAGATCGGCGGGGGTTTGGAT‑GCCCCCCTTTTTCGATAATTTTTTTGCCTCCCGCTTTTGGCCGCGCCGGgcccc > 1:213893/1‑90 (MQ=255) gCCGGAGATAATCGAGATCGGCGGGGGGTGGGAT‑GCCCCCCTTTTTTTATAATTTTTTTGCCCCCCGCCGTAGCCCGCGCCGGccccgtc > 1:545567/1‑87 (MQ=255) tCGAGATCGGCGGGGGTTCGAAT‑GCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACcgcgc < 2:347311/90‑1 (MQ=255) agaTCGGCGGGGGGTCGAAT‑GCCCCCCTTTTTTAATAATGTTTTCGCCTCCCGCTGTAGCCAGCGCCGGCCCCCCCCCCCCCCCCCCGAt > 2:1625643/1‑90 (MQ=255) aTCGGCGGGGGTTCGAAT‑GCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGc < 1:466187/90‑1 (MQ=255) gggggTTCGAAT‑GCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGtt < 1:247315/90‑1 (MQ=255) gAAT‑GCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGGTGCAACGTTGGTATCGc > 1:80396/1‑90 (MQ=255) gCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGtt < 1:605883/90‑1 (MQ=255) | GCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAAT‑GCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTT > NZ_CP009273/642132‑642299 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |