Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP009273765,6750AC59.0% 36.3 / 45.3 39T748P (ACC→CCC) mngBmannosylglycerate hydrolase
Reads supporting (aligned to +/- strand):  ref base A (12/4);  new base C (0/23);  total (12/27)
Fisher's exact test for biased strand distribution p-value = 4.65e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.89e-06
Rejected as consensus: Frequency below/above cutoff threshold.

GGGCGGCCTTCGGGAATTAAAATGCCAGTCCCGGACTCACAACTACGTGGTCTGCTTTCTTGTCGCCTAAGTTTATTGAGTTATACCGGTACGCCAACCGCCGCTGGTGTAGCTCAGCAGGCGCGAGCATGGCTGACTCCAGTACAGTGTTACAACAAAATCCCATGGGA  >  NZ_CP009273/765591‑765760
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gggCGGCCTTCGGGAATTAAAATGCCAGTCCCGGACTCACAACTACGTGGTCTGCTTTCTTGTCGCCTAAGTTTATTGAGTTATACCGGt                                                                                  >  1:47006/1‑90 (MQ=255)
gggCGGCCTTCGGGAATTAAAATGCCAGTCCCGGACTCACAACTACGTGGTCTGCTTTCTTGTCGCCTAAGTTTATTGAGTTATACCGGt                                                                                  >  1:269697/1‑90 (MQ=255)
               aTTAAAATGCCAGCCCCGGACCCACACCTACGTGGTCTGTTTTTTTGCCGCCTAATTTTATTGATTTATCCCGGTCCGCCAACCGCCGCt                                                                   <  1:881016/90‑1 (MQ=255)
                  aaaaTCCCCGCCCCGGCCCCACAACTCCGTGGTTTGTTTTTTTGCCCCCAAATTTTATTGATTTATCCCGGTACGCCAACCGCCGCTGgt                                                                <  1:578855/90‑1 (MQ=255)
                   aaaTCCCAGCCCCGGACTCACAATTACGTGGTCTGCTTTCTTGTCGCCTAAGTTTATGGATTTATCCCGGAACCCCACCCGCCGCTGgtg                                                               <  1:928052/90‑1 (MQ=255)
                         cAGTCCCGGACTCACAACTACGTGGTCTGCTTTCTTGTCGCCTAAGTTTATTGAGTTATACCGGTACGCCAACCGCCGCTGGTGTAGCTc                                                         >  2:1607823/1‑90 (MQ=255)
                          aGTCCCGGACTCACAACTACGTGGTCTGCTTTCTTGTCGCCTAAGTTTATTGAGTTATACCGGTACGCCAACCGCCGCTGGTGTAGCTca                                                        >  1:1307686/1‑90 (MQ=255)
                              ccGGACTCACAACTACGTGGTCTGCTTTCTTGTCGCCTAAGTTTATTGAGTTATACCGGTACGCCAACCGCCGCTGGTGTAGCTcagcag                                                    <  2:399715/90‑1 (MQ=255)
                                ggACTCACAACTACGTGGTCTGCTTTCTTGTCGCCTAAGTTTATTGAGTTATACCGGTACGCCAACCGCCGCTGGTGTAGCTCAGCAGgc                                                  >  1:1264462/1‑90 (MQ=255)
                                 ggcTCACATCTACGTGGTCTGTTTTCTTGGCGCCTAAGTTTATTGAGTTATACCGGGCCCCCAACCGCCGCTGGTGTAGCTCAGCAGgcg                                                 <  2:186473/88‑1 (MQ=255)
                                      aaaaCTCCGGGGTCTGTTTTTTTGTCGCCTAATTTTATTGAGTTATCCCGGTCCGCCAACCGCCGCTGGTGTAGCTCAGCAGGCGCGAGc                                            <  1:118070/88‑1 (MQ=255)
                                        aaCTACGTGGTCTGTTTTCTTGTCGCCTAATTTTATTGAGTTATCCCGGTACGCCAACCGCCGCTGGTGTAGCTCAGCAGGCGCGAGCAt                                          <  2:1043805/90‑1 (MQ=255)
                                        aaCTACGTGGTCTGTTTTCTTGTCGCCAAATTTTATTGAGTTATCCCGGTCCCCCACCCGCCGCTGGTGTAGCTCAGCAGGCGCGAGCAt                                          <  2:1307686/90‑1 (MQ=255)
                                             gggggTCGGCTTTTGTGCCGCCTAATTTTTTTGGTTTATCCCGGTGCGCCACCCGCCGCTGGTGTAGCTCAGCAGGCGCGAGCATGGCTg                                     <  1:949807/87‑1 (MQ=255)
                                             cggggTCTGTTTTCTTGTCGCCTAATTTTATTGAGTTATCCCGGTCCCCCAACCGCCGCTGGTGTAGCTCAGCAGGCGCGAGCATGGCTg                                     <  2:1186025/87‑1 (MQ=255)
                                             cGTGGTCTGTTTTCTTGTCGCCTAATTTTTTTGAGTTATCCCGGTACGCCAACCGCCGCTGGTGTAGCTCAGCAGGCGCGAGCATGGCTg                                     <  1:1607823/90‑1 (MQ=255)
                                             cGTGGTCTGTTTTCTTGTCCCCTAAGTTTATTGAGTTATCCCGGTACGCCAACCGCCGCTGGTGTAGCTCAGCAGGCGCGAGCATGGCTg                                     <  2:297284/90‑1 (MQ=255)
                                             cGTGGTCTGCTTTTTTGTCGCCTAAGTTTTTTGATTTATCCCGGTCCGCCAACCGCCGCTGGTGTAGCTCAGCAGGCGCGAGCATGGCTg                                     <  2:1139143/90‑1 (MQ=255)
                                             cGTGGTCTGCTTTCTTGTCGCCTAATTTTATTGAGTTATACCGGTACGCCAACCGCCGCTGGTGTAGCTCAGCAGGCGCGAGCATGGCTg                                     <  2:481318/90‑1 (MQ=255)
                                             cGTGGTCTGCTTTCTTGTCGCCTAAGTTTATTGAGTTATCCCGGTACGCCAACCGCCGCTGGTGTAGCTCAGCAGGCGCGAGCATGGCTg                                     <  2:271768/90‑1 (MQ=255)
                                             cGTGGTCTGCTTTCTTGTCGCCTAAGTTTATTGAGTTATCCCGGTACGCCAACCGCCGCTGGTGTAGCTCAGCAGGCGCGAGCATGGCTg                                     <  2:163783/90‑1 (MQ=255)
                                             cGTGGTCTGCTTTCTTGTCGCCTAAGTTTATTGAGTTATCCCGGTACGCCAACCGCCGCTGGTGTAGCTCAGCAGGCGCGAGCATGGCTg                                     <  2:396460/90‑1 (MQ=255)
                                             cGTGGTCTGCTTTCTTGTCGCCTAAGTTTATTGAGTTATCCCGGTACGCCAACCGCCGCTGGTGTAGCTCAGCAGGCGCGAGCATGGCTg                                     <  1:628596/90‑1 (MQ=255)
                                             cGTGGTCTGCTTTCTTGTCGCCTAAGTTTATTGAGTTATACCGGTACGCCAACCGCCGCTGGTGTAGCTCAGCAGGCGCGAGCATGGcct                                     >  1:186473/1‑88 (MQ=255)
                                               gggTCTCTTTTCTTGTCGCCAAATTTTATTGAGTTATCCCGGTCCGCCACCCGCCGCTGGTGTAGCTCAGCAGGCGCGAGCATGGCTGAc                                   <  1:1471520/89‑1 (MQ=255)
                                                 gTCTGCTTTCTTGTCGCCTAAGTTTATTGAGTTATACCGGTACGCCAACCGCCGCTGGTGTAGCTCAGCAGGCGCGAGCATGGCTGACTc                                 >  1:1504306/1‑90 (MQ=255)
                                                        ttCTTGTCGCTTAATTTTATTGATTTATCCCGGTACGCCAACCGCCGCTGGTGTAGCTCAGCAGGCGCGAGCATGGCTGACTCCAGTACa                          <  2:713101/90‑1 (MQ=255)
                                                        ttCTTGTCGCCTAATTTTTTTGATTTATCCCGGTACGCCAACCGCCGCTGGTGTAGCTCAGCAGGCGCGAGCATGGCTGACTCCAGTACa                          <  2:1504306/90‑1 (MQ=255)
                                                        ttCTTGTCGCCTAAGTTTATTGAGTTATCCCGGTACGCCAACCGCCGCTGGTGTAGCTCAGCAGGCGCGAGCATGGCTGACTCCAGTACa                          <  2:304563/90‑1 (MQ=255)
                                                        ttCTTGCCCCCTAATTTTATTGATTTATCCCGGTACGCCAACCGCCGCTGGTGTAGCTCAGCAGGCGCGAGCATGGCTGACTCCAGTACa                          <  1:255797/90‑1 (MQ=255)
                                                           ttGTCGCCTAAGTTTATTGAGTTATACCGGTACGCCAACCGCCGCTGGTGTAGCTCAGCAGGCGCGAGCATGGCTGACTCCAGTACAgtg                       >  1:263818/1‑90 (MQ=255)
                                                               cGCCTAAGTTTATTGAGTTATACCGGTACGCCAACCGCCGCTGGTGTAGCTCAGCAGGCGCGAGCATGGCTGACTCCAGTACAGTGTTac                   >  2:1600742/1‑90 (MQ=255)
                                                               cGCCTAAGTTTATTGAGTTATACCGGTACGCCAACCGCCGCTGGTGTAGCTCAGCAGGCGCGAGCATGGCTGACTCCAGTACAGTGTTac                   >  1:1328306/1‑90 (MQ=255)
                                                                        ttATTGAGTTATACCGGTACGCCAACCGCCGCTGGTGTAGCTCAGCAGGCGCGAGCATGGCTGACTCCAGTACAGTGTTACAACAAAATc          <  2:263818/90‑1 (MQ=255)
                                                                          aTTGAGTTATACCGGTACGCCAACCGCCGCTGGTGTAGCTCAGCAGGCGCGAGCATGGCTGACTCCAGTACAGTGTTACAACAAAATccc        >  1:1360346/1‑90 (MQ=255)
                                                                            ttatttATCCCGCTCCCCCAACCGCCGCTGGTGTAGCTCAGCAGGCGCGAGCATGGCTGACTCCAGTACAGTGTTACAACAAAATCCCAt      <  2:22739/86‑1 (MQ=255)
                                                                              aGTTATCCCGGTCCCCCACCCGCCGCTGGTGTAGCTCAGCAGGCGCGAGCATGGCTGACTCCAGTACAGTGTTACAACAAAATCCCATgg    <  1:448180/90‑1 (MQ=255)
                                                                               gTTATACCGGTACGCCAACCGCCGCTGGTGTAGCTCAGCAGGCGCGAGCATGGCTGACTCCAGTACAGTGTTACAACAAAATCCCATggg   >  2:434425/1‑90 (MQ=255)
                                                                                ttATCCCGGTACGCCAACCGCCGCTGGTGTAGCTCAGCAGGCGCGAGCATGGCTGACTCCAGTACAGTGTTACAACAAAATCCCATGGGa  <  2:117173/90‑1 (MQ=255)
                                                                                    |                                                                                     
GGGCGGCCTTCGGGAATTAAAATGCCAGTCCCGGACTCACAACTACGTGGTCTGCTTTCTTGTCGCCTAAGTTTATTGAGTTATACCGGTACGCCAACCGCCGCTGGTGTAGCTCAGCAGGCGCGAGCATGGCTGACTCCAGTACAGTGTTACAACAAAATCCCATGGGA  >  NZ_CP009273/765591‑765760

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: