| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 892,802 | 0 | T | G | 54.5% | 10.6 / 12.6 | 11 | V88G (GTG→GGG) | potI | putrescine ABC transporter permease PotI |
| Reads supporting (aligned to +/- strand): ref base T (0/5); new base G (6/0); total (6/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.16e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.28e-03 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TGACGCGATGATGAGTGCGGTTGGTTTAAGTCTGACAATTGCGGCCTGTGCGGCGACGGCGGCGGCGATCCTCGGAACTATTGCGGCGGTGGTGCTGGTGCGCTTTGGCAGGTTTCGCGGATCAAATGGCTTTGCCTTTATGATCACCGCGCCGCTGGTAATGCCAGATGTCATCACGG > NZ_CP009273/892713‑892891 | tgacgcgatgaTGAGTGCGGTTGGTTTAAGTCTGACAATTGCGGCCTGTGCGGCGACGGCGGCGGCGATCCTCGGAACTATTGCGGCggt < 1:1363646/90‑1 (MQ=255) cgcgatgaTGAGTGCGGTTGGTTTAAGTCTGACAATTGCGGCCTGTGCGGCGACGGCGGCGGCGATCCTCGGAACTATTGCGGCggtggt < 1:617605/90‑1 (MQ=255) ggttTAAGTCTGACAATTGCGGCCTGTGCGGCGACGGCGGCGGCGATCCTCGGAACTATTGCGGCGGGGGTGCTGGGGGGCTTTTGGAgg > 2:792264/1‑90 (MQ=255) aGTCTGACAATTGCGGCCTGTGCGGCGACGGCGGCGGCGATCCTCGGAACTATTGCGGCGGTGGTGCTGGTGCGCTTTGGTAGGTTTcgc < 1:1417495/90‑1 (MQ=255) gTCTGACAATTGCGGCCTGTGCGGCGACGGCGGCGGCGATCCTCGGAACTATTGCGGCGGGGGGGGTGGGGGGCCTTTGTTGGTTTtgcg > 2:1417495/1‑90 (MQ=255) cTGACAATTGCGGCCTGTGCGGCGACGGCGGCGGCGATCCTCGGAACTATTGCGGCGGGGGGGGTGGGGGGCGTTGGGAGGGTTTGCggg > 1:1616948/1‑89 (MQ=255) gACAATTGCGGCCTGTGCGGCGACGGCGGCGGCGATCCTCGGAACTATTGCGGCGGGGGTGGTGGGGAGCCTTTGCCGGGTTCGCggggc > 2:1187531/1‑87 (MQ=255) gACAATTGCGGCCTGTGCGGCGACGGCGGCGGCGATCCTCGGAACTATTGCGGCGGGGGGGGTGGGGGGGTTTGGGGGGGTTTGCCGATc > 1:1582860/1‑90 (MQ=255) gCCTGTGCGGCGACGGCGGCGGCGATCCTCGGAACTATTGCGGCGGTGGTGCTGGTGCGCTTTGGCAGGTTTCGCGGATCAAATGGCttt < 1:219672/90‑1 (MQ=255) gCCTGTGCGGCGACGGCGGCGGCGATCCTCGGAACTATTGCGGCGGTGGTGCTGGTGCGCTTTGGCAGGTTTCGCGGATCAAATGGCttt < 2:1406191/90‑1 (MQ=255) gtgCGGCGACGGCGGCGGCGATCCTCGGAACTATTGCGGCGGTGGTGCTGGTGCGCTTTGGCAGGTTTCGCGGATCAAATGGCTTTGCCt < 2:1582860/90‑1 (MQ=255) ggcggcggcGATCCTCGGAACTATTGCGGCggtggt > 2:847093/1‑36 (MQ=255) ggcggcggcGATCCTCGGAACTATTGCGGCggtggt < 1:847093/36‑1 (MQ=255) ggcggcggcGATCCTCGGAACTATTGCGGCGgtggtg < 2:838759/37‑1 (MQ=255) ggcggcggcGATCCTCGGAACTATTGCGGCGggggtg > 1:838759/1‑37 (MQ=255) ggcggcggcGATCCTCGGAACTATTGCGGCGGGGGGGCTGGGGCGCCTTGGGAGGGTTTGCCGGTCAAAAGGGTTTGGCCTTTTTATCAc > 1:33967/1‑90 (MQ=255) cgATCCTCGGAACTATTGCGGCGGTGGTGCTGGTGCGCTTTGGCAGGTTTCGCGGATCAAATGGCTTTGCCTTTATGATCACCGcgccgc < 1:108644/90‑1 (MQ=255) ggtggtGCTGGTGCGCTTTGGCAGGTTTCGCGGATCAAATGGCTTTGCCTTTATGATCACCGCGCCGCTGGTAATGCCAGATGTCATCAc > 1:442228/1‑90 (MQ=255) ggtggtGCTGGTGCGCTTTGGCAGGTTTCGCGGATCAAATGGCTTTGCCTTTATGATCACCGCGCCGCTGGTAATGCCAGATGTCATCAc < 1:1038647/90‑1 (MQ=255) tggtgctggtgcGCTTTTGCAGGTTTCGCGGGTCAAAAGGCTTTGCCTTTTTTATCACCGCGCCGCTGGGAATGCCAGATGTCATCACgg > 1:36461/1‑90 (MQ=255) | TGACGCGATGATGAGTGCGGTTGGTTTAAGTCTGACAATTGCGGCCTGTGCGGCGACGGCGGCGGCGATCCTCGGAACTATTGCGGCGGTGGTGCTGGTGCGCTTTGGCAGGTTTCGCGGATCAAATGGCTTTGCCTTTATGATCACCGCGCCGCTGGTAATGCCAGATGTCATCACGG > NZ_CP009273/892713‑892891 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |