| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 970,375 | 0 | A | G | 61.1% | 12.1 / 17.0 | 18 | I201V (ATT→GTT) | mukF | chromosome partition protein MukF |
| Reads supporting (aligned to +/- strand): ref base A (1/6); new base G (11/0); total (12/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.77e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.50e-04 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CTGACGCAACGTCTGATGGACGAACAGCAGCAGCAGGTGAAGGACGATATCGCCCAGTTGCTGAACAAAGACTGGCGGGCGGCGATTTCCAGCTGTGAATTGTTGCTTTCGGAAACTTCCGGAACGCTGCGTGAATTGCAGGATACGCTGGAAGCGGCAGGC > NZ_CP009273/970291‑970452 | cTGACGCAACGTCTGATGGACGAACAGCAGCAGCAGGTGAAGGACGATATCGCCCAGTTGCTGAACAAAGACTGGCGGGCGGCGATTTcc < 1:108089/90‑1 (MQ=255) gACGCAACGTCTGATGGACGAACAGCAGCAGCAGGTGAAGGACGATATCGCCCAGTTGCTGAACAAAGACTGGCGGGGGGGGGGTTCCAg > 1:26467/1‑90 (MQ=255) gACGCAACGTCTGATGGACGAACAGCAGCAGCAGGTGAAGGACGATATCGCCCAGTTGCTGAACAAAGACTGGCGGGCGGGGGTTTTCAg > 2:814744/1‑90 (MQ=255) aCGTCTGATGGACGAACAGCAGCAGCAGGTGAAGGACGATATCGCCCAGTTGCTGAACAAAGACTGGCGGGCGGCGGTTTCCAGCtgtgg > 2:448259/1‑89 (MQ=255) cGAACAGCAGCAGCAGGTGAAGGACGATATCGCCCAGTTGCTGAACAAAGACTGGCGGGGGGGGGTTTTCAGCTGTGAATTGTTGttttt > 1:644068/1‑89 (MQ=255) gTGAAGGACGATATCGCCCAGTTGCTGAACAAAGACTGGCGGGGGGGGGTTTCCCGCTGTGAATTGTTTTTTTTGGGAAATTCCgggaag > 1:70671/1‑86 (MQ=255) gTGAAGGACGATATCGCCCAGTTGCTGAACAAAGACTGGCGGGCGGCGATTTCCAGCTGTGAATTGTTGCTTTCGGAAACTTCCGGAACg < 2:624064/90‑1 (MQ=255) tGAAGGACGATATCGCCCAGTTGCTGAACAAAGACTGGCGGGCGGCGATTTCCAGCTGTGAATTGTTGCTTTCGGAAACTTCCGGAACGc < 1:887512/90‑1 (MQ=255) gAAGGACGATATCGCCCAGTTGCTGAACAAAGACTGGCGGGCGGCGATTTCCAGCTGTGAATTGTTGCTTTCGGAAACTTCCGGAACGCt < 2:567268/90‑1 (MQ=255) aaGGACGATATCGCCCAGTTGCTGAACAAAGACTGGCGGGGGGGGGCTTCCCACTGTGGATTGTTGTTTTCGGAAACCTCCCGAAAGccg > 1:624064/1‑88 (MQ=255) ggACGATATCGCCCAGTTGCTGAACAAAGACTGGCGGGCGGCGATTTCCAGCTGTGAATTGTTGCTTTCGGAAACTTCCGGAACGCTGCg < 2:1457217/90‑1 (MQ=255) cGATATCGCCCAGTTGCTGAACAAAGACTGGCGGGCGGCGATTTCCAGCTGTGAATTGTTGCTTTCGGAAACTTCCGGAACGCTGCGTGa < 1:1249586/90‑1 (MQ=255) gATATCGCCCAGTTGCTGAACAAAGACTGGCGGGCGGCGGTTTTCAGCTGTGAATTGTTTCTTTTGGAAAATTCCCGGACGCCGCGGGaa > 2:860243/1‑90 (MQ=255) gTTGCTGAACAAAGACTGGCGGGCGGCGGTTTTCAGCTGTGGATTTTTTCTTTTGGAAAATTCCGGGAAGCCGCGGGGATTGTAGGGTAc > 1:532373/1‑90 (MQ=255) tGAACAAAGACTGGCGGGCGGGGGTTTTCAGCTTTGGATTTTTGTTTTTGGAAAATTTCGGGAAGCGGCGGGAATTGGAGGATGCGCTgg > 1:777680/1‑90 (MQ=255) aaaGACTGGCGGGCGGCGGGTTTCCCCTGGGAATTGTTTCTTTTTGAAACCTCCCGGAAGCTGGGGGGATTGTAGGATAAGCCGGAAGCg > 1:1162882/1‑90 (MQ=255) aaaGACTGGCGGGCGGCGATTTCCCGCTGTGAATTGTTGCTTTCGGAAAATTCCGGAACGCTGCGGGAATTGTAGGATAAGCTGGAAggg > 2:1499606/1‑88 (MQ=255) tGGCGGGCGGCGGTTTCCAGCCGTGAAATGTTTCTTTCGGAAACTTCCGGGAAGCTGCGGGGATTGCAGGGTACGCTGGAAGcggccggc > 2:1328587/1‑90 (MQ=255) | CTGACGCAACGTCTGATGGACGAACAGCAGCAGCAGGTGAAGGACGATATCGCCCAGTTGCTGAACAAAGACTGGCGGGCGGCGATTTCCAGCTGTGAATTGTTGCTTTCGGAAACTTCCGGAACGCTGCGTGAATTGCAGGATACGCTGGAAGCGGCAGGC > NZ_CP009273/970291‑970452 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |