Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092731,620,6540GC58.3% 5.9 / 14.2 12G328G (GGC→GGGdcppeptidyl‑dipeptidase Dcp
Reads supporting (aligned to +/- strand):  ref base G (5/0);  new base C (0/7);  total (5/7)
Fisher's exact test for biased strand distribution p-value = 1.26e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.93e-03
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

CCCGCCGTACCTGTTCCGCATAAAATGCCCAGTCCCACGGCTGCGCGCTAAACCCGCCCTGCTGCTTATCGATAACCGCCTGTATGGAGGCTAATTCATCGCTCGCACGTTGACGCGCCGCTGGAACAATTTCCCGCATA  >  NZ_CP009273/1620599‑1620738
                                                       |                                                                                    
cccGCCGTACCTGTTCCGCATAAAATGCCCAGTCCCACGGCTGCGCGCTAAACCCGCCCTGCTGCTTATCGATAACCGCCTGTATGGAgg                                                    >  1:1479636/1‑90 (MQ=255)
   cccgTCCCTGTTCCGCAAAAAATCCCCAGCCCCCCGGCTGCGCGCAAACCCCCCCCTGCTGCTTATCGATAACCGCCTGTATGGAGGCTa                                                 <  2:756932/89‑1 (MQ=255)
          cTGTCCCGCAAAAAATCCCCCCCCCCCCGGCGGCGCGCAACCCCCCCCCTGCTGTTTATCGAAAACCGCCTGTATGGAGGCTAATTCAtc                                          <  1:935336/90‑1 (MQ=255)
            gTTCCGCATAAAATGCCCAGTCCCACGGCTGCGCGCTAAACCCGCCCTGCTGCTTATCGATAACCGCCTGTATGGAGGCTAATTCAtcgc                                        >  2:1458761/1‑90 (MQ=255)
                  aaaaaaaTGCCCAGCCCCACGGCTGCGCGCAAACCCCCCCCTGCTGCTTATCGATAACCGCCTGTATGGAGGCTAATTCATCGCTCGCAc                                  <  2:197396/87‑1 (MQ=255)
                                ccccAGGGCTGCGCGCAAACCCCCCCCTGCTGCTTATCGATAACCGCCTGTATGGAGGCTAATTCATCGCTCGCACGTTGACGCGCCGCt                    <  2:1479636/89‑1 (MQ=255)
                                  cccgggCTGCGCGTAAACCCCCCCCTGCTGTTTATCGATAACCGCCTGTATGGAGGCTAATTCATCGCTCGCACGTTGACGCGCCGCTgg                  <  2:782601/86‑1 (MQ=255)
                                         tGCGCGCTAAACCCGCCCTGCTGCTTATCGATAACCGCCTGTATGGAGGCTAATTCATCGCTCGCACGTTGACGCGCCGCTGGAACAAtt           >  1:239968/1‑90 (MQ=255)
                                         tGCGCGCTAAACCCGCCCTGCTGCTTATCGATAACCGCCTGTATGGAGGCTAATTCATCGCTCGCACGTTGACGCGCCGCTGGAACAAtt           >  1:819324/1‑90 (MQ=255)
                                         tGCGCGCTAAACCCGCCCTGCTGCTTATCGATAACCGCCTGTATGGAGGCTAATTCATCGCTCGCACGTTGACGCGCCGCTGGAACAAtt           >  2:1273543/1‑90 (MQ=255)
                                            gcgcAAACCCCCCCCTGCTGCTTATCAATAACCGCCTGTATGGAGGCTAATTCATCGCTCGCACGTTGACGCGCCGCTGGAACAATTTcc        <  2:1211136/90‑1 (MQ=255)
                                            ccgcAAACCCCCCCCTGTTCTTTATCAAAAACCGCCTGTATGGAGGCTAATTCATCGCTCGCACGTTGACGCGCCGCTGGAACAATTTcc        <  2:950606/89‑1 (MQ=255)
                                                  acccccccCTGCTGTTTATCGAAACCCGCCTGTATGGAGGCTAATTCATCGCTCGCACGTTGACGCGCCGCTGGAACAATTTCCCGCATa  <  2:777645/88‑1 (MQ=255)
                                                       |                                                                                    
CCCGCCGTACCTGTTCCGCATAAAATGCCCAGTCCCACGGCTGCGCGCTAAACCCGCCCTGCTGCTTATCGATAACCGCCTGTATGGAGGCTAATTCATCGCTCGCACGTTGACGCGCCGCTGGAACAATTTCCCGCATA  >  NZ_CP009273/1620599‑1620738

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: