| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,819,318 | 0 | G | A | 57.1% | 1.9 / 42.3 | 35 | intergenic (‑124/+79) | ves/spy | environmental stress‑induced protein Ves/ATP‑independent periplasmic protein‑refolding chaperone Spy |
| Reads supporting (aligned to +/- strand): ref base G (13/2); new base A (0/20); total (13/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.11e-08 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.56e-06 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAA‑C‑TGTTTG‑AGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAG > NZ_CP009273/1819238‑1819395 | cggTGGTTGGTTATCGGCAACTGGGGTGTTTTACGTTAGTGGTGATCAGGTTTTTTTTACAA‑T‑TGTTTG‑AAAAGAAAAAAAAAAAccgcc < 1:224875/88‑1 (MQ=255)cggTGGATGTTTATCGGCAACTGGGGTGATTTACGTTAGTGGTGATCGGGTTTTTTTTACAA‑C‑TGTTTG‑AGAAGAAAAAAAAAAAccgcc < 2:138334/88‑1 (MQ=255) ggATGTTTACCGGCAACTGGGGTAATTTACGTTAGTGGTGATCAGGCTTTTTTTACAA‑C‑TGTTTG‑AGAAGAGAAAAGAAAACCGCCGATc < 1:206781/90‑1 (MQ=255) ggATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAA‑C‑TGTTTG‑AGAAGAGAAAAGAAAACCGCCGATc > 2:110255/1‑90 (MQ=255) gATGGTTATCGGCAACTCGGGTGTTTTACGTTAGGGGTGATCAGGTTTTTTTTACAA‑C‑TGTTTG‑AGAAGAAAAAAAAAAACCGCCGATcc < 2:10839/90‑1 (MQ=255) gTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAA‑C‑TGTTTG‑AGAAGAGAAAAGAAAACCGCCGATCCTGTc > 2:1457905/1‑90 (MQ=255) ttATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAA‑C‑TGTTTG‑AGAAGAGAAAAGAAAACCGCCGATCCTGTcc > 2:907313/1‑90 (MQ=255) aTCGGCACCGGGGGTGATTTACGTTGGGGGTGATCGGGTTTTTTTTACAA‑T‑TTTTGG‑AGAAGAAAAAAAAAAACCGCCGATCCTGTCCAc < 1:256944/90‑1 (MQ=255) tCGGCAACTGGGGTTTTTTACTTTGGTGGTGATCGGGTTTTTTTTACAC‑T‑TGTTTG‑AAAAGAAAAAAAAAAACCGCCGATCCTGTCCAcc < 2:1392731/90‑1 (MQ=255) agcTCGGTTTATTTACGTTAGTGGTGATCGGGTTTTTTTTACAC‑C‑TTTTTG‑AGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCAtt < 2:335539/88‑1 (MQ=255) aaCTGGGGTAATTTACGTTAGTGGTGATCAGGTTTTTTTTACAA‑C‑TTTTTG‑AAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCAtt < 2:1103786/90‑1 (MQ=255) aaCTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAA‑C‑TGTTTG‑AGAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCAtt < 2:204894/90‑1 (MQ=255) aCTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAA‑C‑TGTTTG‑AGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTa > 2:1012247/1‑90 (MQ=255) aCTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAA‑C‑TGTTTG‑AGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTa > 1:169832/1‑90 (MQ=255) cgggggTGTTTTACGTTAGTGGTAATCAGTTTTTTTTAAAAA‑T‑TTTTTG‑AGAAAAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTAc < 1:907313/87‑1 (MQ=255) gggTGATTTCCTTTGGGGGTGA‑CCGGTTTTTTTTAAAACC‑TTTTTG‑AAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGc < 1:504265/90‑1 (MQ=255) gttttttCCGTTAGTGGTGATCAGGTTTTTTTTACAA‑C‑TTTTTG‑AAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCaa < 2:1488369/86‑1 (MQ=255) gttaTTTACGTTAGGGGTGATCGGGTTTTTTTTACAA‑C‑TTTTTG‑AAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCaa < 1:1186488/87‑1 (MQ=255) gtgtTTTAGGTTGGTGGTGATC‑GGCTTTTTTTTAAA‑CTTTTTTG‑AGAAGAAAAAAAAAACCCGCCGATCCTGTCCACCGCATTACTGCaa < 1:1248863/90‑1 (MQ=255) gtgtTTTACGTTAGTGGTGATCAGGTTTTTTTTACAA‑T‑TGTTTG‑AGAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCaa < 2:991450/90‑1 (MQ=255) gTGATTTACGTTGGGGGTGATCAGTTTTTTTTTACAA‑T‑TTTTGG‑AGAAAAAAAAAAAAACCCGCCGATCCTGTCCACCGCATTACTGCaa < 1:1012247/90‑1 (MQ=255) aggATTTACGTTAGTGGTGATCGGTTTTTTTTAACAA‑T‑TTTTTG‑AAAAGAAAAAAAAAACCCGCCGATCCTGTCCACCGCATTACTGCaa < 1:1262781/88‑1 (MQ=255) ttttACGTTAGTGGTGATCGGTTTTTTTTTAC‑A‑C‑TTTTTGAAAAAGAAAAAAAAAACCCGCCGATCCTGTCCACCGCATTACTGCAAGGt < 1:1457905/89‑1 (MQ=255) aTTTACTTTAGGGGTCACCAGGTTTTTTTTCCAT‑T‑TTTTTA‑AAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGt < 1:1262222/90‑1 (MQ=255) ttACGTTAGTGGTGATCAGGCTTTTTTTACAA‑C‑TGTTTG‑AGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGtct > 1:335539/1‑88 (MQ=255) cagcGTTAGTGGTGATCGGTTTTTTTTTACAC‑C‑TTTTTG‑AAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAg < 1:383823/87‑1 (MQ=255) aGTGGTGATCAGGCTTTTTTTACAA‑C‑TGTTTG‑AGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGAcct > 2:1262781/1‑88 (MQ=255) aGTGGTGATCAGGCTTTTTTTACAA‑C‑TGTTTG‑AGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACaa > 2:891260/1‑90 (MQ=255) gtggtgATCAGGCTTTTTTTACAA‑C‑TGTTTG‑AGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAg > 1:1204525/1‑90 (MQ=255) gATCAGGCTTTTTTTACAA‑C‑TGTTTG‑AGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGAcctg > 2:383823/1‑88 (MQ=255) ggCTTTTTTTACAA‑C‑TGTTTG‑AGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACcggcgggc > 1:1596959/1‑88 (MQ=255) ggCTTTTTTTACAA‑C‑TGTTTG‑AGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTc > 1:646040/1‑90 (MQ=255) tttttACAA‑C‑TGTTTG‑AGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAg > 2:1305503/1‑90 (MQ=255) tttttG‑AAAAAAAAAAAAAAACCCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCt < 2:795560/88‑1 (MQ=255) g‑agaagaaaaAAAAAACCCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAg < 1:1525641/90‑1 (MQ=255) agaaaagaaaACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACcggc > 1:107447/1‑60 (MQ=255) aaaaaagaaaaCCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACcggc < 2:107447/58‑1 (MQ=255) | CCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAA‑C‑TGTTTG‑AGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAG > NZ_CP009273/1819238‑1819395 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |