| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,380,341 | 0 | G | A | 63.6% | ‑4.0 / 21.1 | 22 | intergenic (+31/‑72) | yfbL/yfbM | M28 family metallopeptidase/YfbM family protein |
| Reads supporting (aligned to +/- strand): ref base G (1/7); new base A (14/0); total (15/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.69e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.79e-05 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCT > NZ_CP009273/2380262‑2380428 | ggCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGcac < 1:1008974/90‑1 (MQ=255) tAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACattttatt < 1:1031082/90‑1 (MQ=255) tGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGc < 1:61980/77‑1 (MQ=255) tGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAACGACATGc > 2:61980/1‑77 (MQ=255) ggTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGGCCAGAAAAAAAGACAAGCACATTTTTTTTTTCCTcc > 2:101638/1‑89 (MQ=255) ggTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGGCCAGAAAAAAAGACAAGCACAATTTTTTTTTCCTcc > 2:196697/1‑89 (MQ=255) gtgtTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGGCCCGAAAAAAAGAAAAGCAAAATTTTTTTTTTCTCAt > 1:349564/1‑90 (MQ=255) tgtTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATc < 1:108482/90‑1 (MQ=255) ttATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTg < 2:16363/90‑1 (MQ=255) tttGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTGCCGAAAAAACAACATGCACACTTTTTTTTTTCTCATCTTTTTTTAg > 2:986455/1‑90 (MQ=255) tttGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAAAAAAAACGAGAAACACAATTTTTTTTTTCCCATATGtttttca > 2:1421190/1‑87 (MQ=255) tttGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGGCCAGAAAAAACGACATGCACATTTTTTTTTTCCTCCTCTGtttttaa > 2:322746/1‑87 (MQ=255) tttGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGGCCAGAAAAAACGACAAGCCCATTTTTTTTTTCCTCATCtttttttaa > 2:1359797/1‑87 (MQ=255) tttGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGGCCAGAAAAAAAGAAAAGCACAATTTTTTTTTTCTCCTCtttttttaa > 1:408020/1‑87 (MQ=255) acaGTAAATAATAAAAATTTCTCGAGGCGGGTCCCGAAAAAACGACATGCACATTTTTTTTTTCCTCATCTGTTTTTAAATGCGGAAAAt > 2:118470/1‑90 (MQ=255) gTAAATAATAAAAATTTCTCGAGGCGGGGCCCGAAAAAACGACAAGCACATTTTTTTTTTCCTCCTCTTTTTTTAAAAGCGAAACATGaa > 2:300686/1‑90 (MQ=255) ataaAAATTTCTCGAGGCGGGGCCAGAAAAAACGACATGCACATTTTTTTTTTCCTCATCTGTTTTTAAATGCGGAACATGAAATTTaaa > 2:259193/1‑89 (MQ=255) aaaaTTTCTCGAGGCGGGTCCAGAAAAAACGACAAGCACATTTTTTTTTTCCTCCTCTGTTTTTAAATGGGAAACATGAAATTTAACgga > 2:972013/1‑90 (MQ=255) ttCTCGAGGCGGGGCCCGAAAAAACGACAAGCACAATTTTTTTTTCCCCATCTGTTTTTAAATGCGAAAAAAGAAAATTAACGGAGGgaa > 2:516336/1‑90 (MQ=255) gggTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAAAATAAATggg < 1:1594453/90‑1 (MQ=255) ggTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGa < 2:508344/90‑1 (MQ=255) aaGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCt > 1:21048/1‑90 (MQ=255) | GGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCT > NZ_CP009273/2380262‑2380428 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |