| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 297,075 | 0 | A | C | 52.9% | 20.0 / 23.1 | 17 | V175G (GTG→GGG) | paoB | aldehyde oxidoreductase FAD‑binding subunit PaoB |
| Reads supporting (aligned to +/- strand): ref base A (7/1); new base C (0/9); total (7/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.11e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.10e-04 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GCGGCGTTTTTCCCGGAGGGTGATAAAAATCAGCCAGTGTGATACTGCGAGTCTTTCCCTCCGGCGTGATGGTTTCCACCACCGCATCCAGCAACCGCATTGCGACCGCCATATCGCTCGGATGGGTGGCAATGCAGGCTTCGCTTACGCCTACCACCGCGTGCTG > NZ_CP009273/296995‑297160 | gcggcgTTTTTCCCGGAGGGTGATAAAAATCAGCCAGTGTGATACTGCGAGTCTTTCCCTCCGGCGTGATGGTTTCCACCACCGCATCCa > 1:261296/1‑90 (MQ=255)gcggcgTTTTTCCCGGAGGGTGATAAAAATCAGCCAGTGTGATACTGCGAGTCTTTCCCTCCGGCGTGATGGTTTCCACCACCGCATCCa > 1:508317/1‑90 (MQ=255) tttttCCCGGAGGGTGATAAAAATCAGCCAGTGTGATACTGCGAGTCTTTCCCTCCGGCGTGATGGTTTCCACCACCGCATCCAGCAAcc < 1:95832/90‑1 (MQ=255) cGGAGGGTGATAAAAATCAGCCAGTGTGATACTGCGAGTCTTTCCCTCCGGCGTGATGGTTTCCACCACCGCATCCAGCAACCGCATTGc > 1:533659/1‑90 (MQ=255) gggTAAAAAAAATCAGCCAGTTTGATACTGCGAGTCTTCCCCCCCGGGGTGATGTTTCCCCCCCCCGCACCCAGAACCCGCTTTGCGAcc < 2:508317/90‑1 (MQ=255) aaTCAGCCAGTGTGATACTGCGATTCTTCCCCCCCGGCGTGATGGTTCCCACCCCCGCATCCAGCAACCGCTTTGCGACCGCCATATCGc < 1:564406/90‑1 (MQ=255) aaTCAGCCAGTGTGATACTGCGAGTCTTTCCCTCCGGCGTGATGGTTTCCACCACCGCATCCAGCAACCGCATTGCGACCGCCATATCGc > 2:82322/1‑90 (MQ=255) cAGCCAGTGTGATACGGCGAGTCTTTCCCCCCGGCGTGATGTTTTCCCCCCCCGCATCCAGCACCCGCATTGCGACCGCCATATCGCTCg < 1:379179/90‑1 (MQ=255) gCCGGTGTGATCCTGCGAGTCTTCCCCCCCGGGGTGATGTTTTCCCCCCCCGCACCCAGCACCCGCTTTGCGACCGCCATATCGCTCGGa < 1:63812/90‑1 (MQ=255) gCCAGTGTAATCCTGCGAGTCTTCCCCCCCGGCGTGATGGTTTCCCCCCCCGCATCCAGCAACCGCATTGCGACCGCCATATCGCTCGGa < 1:203925/90‑1 (MQ=255) ccggtgtGATACGGCGAGTCTTCCCCCCCGGCGTGATGGTTTCCCCCCCCGCATCCAGCAACCGCTTTGCGACCGCCATATCGCTCGGAt < 1:267230/87‑1 (MQ=255) aGTGTAATACTGGAGGTCTTCCCCCCCGGGGTGATGTTTCCCCCCCCCGCATCCAACACCCCCATTGCGCCCGCCATATCGCTCGGATgg < 1:484384/90‑1 (MQ=255) gATACTGCGAGTCTTCCCCCCCGGCGTGATGTTTTCCCCCCCCGCATCCAGCACCCGCTTTGCGACCGCCATATCGCTCGGATGGGTGGc < 1:394037/90‑1 (MQ=255) gCGAGTCTTTCCCTCCGGCGTGATGGTTTCCACCACCGCATCCAGCAACCGCATTGCGACCGCCATATCGCTCGGATGGGTGGCAATGCa > 2:115176/1‑90 (MQ=255) gAGTCTTTCCCTCCGGCGTGATGGTTTCCACCACCGCATCCAGCAACCGCATTGCGACCGCCATATCGCTCGGATGGGTGGCAATGCAgg > 2:126309/1‑90 (MQ=255) ttCCCTCCGGCGTGATGGTTTCCACCACCGCATCCAGCAACCGCATTGCGACCGCCATATCGCTCGGATGGGTGGCAATGCAGGCTTCGc > 2:143885/1‑90 (MQ=255) ccTCCGGCGGGTTGTTTTCCCCCCCCCCCCCCCGCACCCACTTTGCACCCGCCATATCGCTCGGATGGGTGGCAATGCAGGCTTCGCTTa < 1:497059/90‑1 (MQ=255) caccacCGCATCCAGCAACCGCATTGCGACCGCCATATCGCTCGGATGGGTGGCAATGCAGGCTTCGCTTACGCCTACCACCGCGTGCTg > 1:520333/1‑90 (MQ=255) | GCGGCGTTTTTCCCGGAGGGTGATAAAAATCAGCCAGTGTGATACTGCGAGTCTTTCCCTCCGGCGTGATGGTTTCCACCACCGCATCCAGCAACCGCATTGCGACCGCCATATCGCTCGGATGGGTGGCAATGCAGGCTTCGCTTACGCCTACCACCGCGTGCTG > NZ_CP009273/296995‑297160 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |