Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP009273822,9550TG58.3% 3.7 / 10.4 12T495P (ACC→CCC) ybhFATP‑binding cassette domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base T (1/4);  new base G (7/0);  total (8/4)
Fisher's exact test for biased strand distribution p-value = 1.01e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.39e-02
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

CATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAACAGGCCAGCGCCAGCCGC  >  NZ_CP009273/822878‑823037
                                                                             |                                                                                  
cATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAAc                                                                        <  2:57100/90‑1 (MQ=255)
       tgggtggTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGGCAACGCCggga                                                                 >  2:165908/1‑88 (MQ=255)
        gggtggTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGGGGGGGGGAACGCCGGGAg                                                                >  2:53528/1‑90 (MQ=255)
                   aTCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGGGGGGGGCAACGCCGGAAGTCGGGtcgtcg                                                     >  1:408072/1‑90 (MQ=255)
                    tCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCga                                                    <  1:418764/90‑1 (MQ=255)
                           gACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGGGGGGGGCAACGCCGGGAGTAGGTTCGTCGGGaaaaaa                                             >  1:596418/1‑87 (MQ=255)
                                  ttCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTcc                                      <  2:391282/90‑1 (MQ=255)
                                           aTGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGGGGGGGGGAAAGCCCGGAGTCGGTTCGTCGGGAAACAAAAAGTCCGGGTCATGc                             >  1:394778/1‑90 (MQ=255)
                                                     tgtgCAGCCAAAATTCACGGCGGGGGGGGGGGGCAACGCCCGAAGGCGGGTCGTCGGGAAAAAAAAAGTCCGGGTTATGCATCAGCGaag                   >  1:433848/1‑89 (MQ=255)
                                                        gCAGCCAAAATTCACGGCGGGGGGGGGGGGCAACGCCGGAAGGCGGGTCGTCGAGAAAAAAAAAGTCCGGGTCATTCATCACCGAACAgg                >  2:245380/1‑90 (MQ=255)
                                                          aGCCAAAATTCACGGCGGGGGGGGGGGGGAACCCCGGGAGGGGGGTCGTCGAGAAAAAGAAAGTCCGGGTCATGGATCAGCGGAAAGgcc              >  2:374414/1‑90 (MQ=255)
                                                                      cggcggGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAACAGGCCAGCGCCAGCCGc  <  1:245380/90‑1 (MQ=255)
                                                                             |                                                                                  
CATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAACAGGCCAGCGCCAGCCGC  >  NZ_CP009273/822878‑823037

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑