| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,152,727 | 0 | T | G | 61.5% | 5.3 / 12.7 | 13 | G165G (GGT→GGG) | ycfH | metal‑dependent hydrolase |
| Reads supporting (aligned to +/- strand): ref base T (0/5); new base G (8/0); total (8/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.77e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.13e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CTGGCAATTCTGCGCGAAGAAAAAGTGACGGATTGCGGTGGCGTACTACACTGTTTTACAGAGGACAGAGAAACGGCGGGTAAATTACTGGATCTCGGATTTTACATCTCCTTTTCCGGCATTGTGACCTTCCGTAATGCGGAGCAACTGCGCGATGCTGCGCGTTATGT > NZ_CP009273/1152647‑1152816 | cTGGCAATTCTGCGCGAAGAAAAAGTGACGGATTGCGGTGGCGTACTACACTGTTTTACAGAGGACAGAGAAACGGCGGGTAAATTACTg < 1:304786/90‑1 (MQ=255) tCTGCGCGAAGAAAAAGTGACGGATTGCGGTGGCGTACTACACTGTTTTACAGAGGACAGAGAAACGGCGGGTAAATTACTGGATCTCgg < 2:111973/90‑1 (MQ=255) gACGGATTGCGGTGGCGTACTACACTGTTTTACAGAGGACAGAGAAACGGCGGGGGAATTATTTGGTCTCGGGTTTTTAATCTCCttttt > 1:447572/1‑89 (MQ=255) ggCGTACTACACTGTTTTACAGAGGACAGAGAAACGGCGGGTAAATTACTGGATCTCGGATTTTACATCTCCTTTTCCGGCATTGTGAcc < 1:365990/90‑1 (MQ=255) gTTTTACAGAGGACAGAGAAACGGCGGGGAAATTACTGGGTCTCGGGTTTTAAAACTCCTTTTTCCGCATTGTGGCCCTCCCTAATGCgg > 1:107000/1‑90 (MQ=255) gTTTTACAGAGGACAGAGAAACGGCGGGGAAAATAATGGGTCTCGGGTTTTAAATCTCCTTTTTCCGCATTGTGACCTTCCCTAATGCgg > 1:479161/1‑90 (MQ=255) gagGACAGAGAAACGGCGGGGAAATTAATTGGTCTTGGGTTTTTTATCTCCTTTTTCTGCATTTTGGCCTTCCCTTATGCGGGGGAACTg > 2:530828/1‑90 (MQ=255) ggACAGAGAAACGGCGGGGAAAATACTTGGTTTCTGGTTTTTCATTTCCTTTTTCCGCCTTGTGGACTTTCCTAATGCGGGGGAACTgcg > 2:545757/1‑90 (MQ=255) ggACAGAGAAACGGCGGGGAAAATACTGGATTTCGGGTTTTTAATCTCCTTTTTCGGGCTTGTGGGCTTTCGTTATGCGGGGGAAcaggg > 1:200327/1‑86 (MQ=255) ggACAGAGAAACGGCGGGGAAAATAATTGATTTCTGGTTTTTTAATTCCTTTTTCCGCATTGGGGCCTTCCCTAATGCGGGGCAACCgcc > 2:268866/1‑89 (MQ=255) ggACAGAGAAACGGCGGGGAAAATAATGGATTTCGGGTTTTAAATCTCCTTTTTCCGCGTTTTGACCTTTCGTAATGCGGGGGAAATgcg > 2:67119/1‑90 (MQ=255) gagaAACGGCGGGTAAATTACTGGATCTCGGATTTTACATCTCCTTTTCCGGCATTGTGACCTTCCGTAATGCGGAGCAACTGCGCGAtg < 1:545757/90‑1 (MQ=255) gTAAATTACTGGATCTCGGATTTTACATCTCCTTTTCCGGCATTGTGACCTTCCGTAATGCGGAGCAACTGCGCGATGCTGCGCGTTATg < 1:268866/90‑1 (MQ=255) tAAATTACTGGATCTCGGATTTTACATCTCCTTTTCCGGCATTGTGACCTTCCGTAATGCGGAGCAACTGCGCGATGCTGCGCGTTATGt < 2:571314/90‑1 (MQ=255) | CTGGCAATTCTGCGCGAAGAAAAAGTGACGGATTGCGGTGGCGTACTACACTGTTTTACAGAGGACAGAGAAACGGCGGGTAAATTACTGGATCTCGGATTTTACATCTCCTTTTCCGGCATTGTGACCTTCCGTAATGCGGAGCAACTGCGCGATGCTGCGCGTTATGT > NZ_CP009273/1152647‑1152816 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |