| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,963,417 | 0 | A | C | 53.3% | 8.7 / 11.0 | 15 | T343P (ACC→CCC) | wzzE | ECA polysaccharide chain length modulation protein |
| Reads supporting (aligned to +/- strand): ref base A (3/4); new base C (0/8); total (3/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.69e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.73e-02 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGTCGGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTACTGA > NZ_CP009273/3963341‑3963505 | cgATGGCCCACGTCGTGCCTTCCTGATGTTTATGTGGGGCATTGTCGGGGGGCTGATCGGGGTTGGTGTCGCATTAACCCGCCGTTGCTc < 2:258511/90‑1 (MQ=255)cgATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGTGGGGGGGCTGATGGGGGCTGGTGTCGCATTAACCCGCCGTTGCTc < 2:425852/90‑1 (MQ=255) cccgtcgtGCCTTCCTGATGATTAGGGGGGGCTTTGTGGGGGGGCTGATGGGGGCTGGTGTCGCATTACCCCGCCGTTGCTCGAAATAGc < 2:576858/88‑1 (MQ=255) gtcgtGCTTTCCTGATGATTATGGGGGGCTTTGTGGGGGGGCTGATCGGGGTTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAac < 2:146444/90‑1 (MQ=255) cgtGCCTTCCTGATGATTATGTGGGGCATTGTCGGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAacac < 1:7246/90‑1 (MQ=255) tgattaTTATGTGGGTCATTGGGGGGGGGCTGCCGGGGGGGGGTGTCGCTTTCCCCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTg < 2:522150/90‑1 (MQ=255) tgatgaTTATGTGGGGCTTTGTCGGGGGGCTGATGGGGGCTGGTGTCGCATTACCCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTg < 1:433342/90‑1 (MQ=255) tgatgaTTATGGGGGGCATTGTCGGGGGGCTGATGGGGGTGGGTGCCGCATTACCCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTg < 1:474752/90‑1 (MQ=255) ttATGTGGGGCATTGTCGGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCa > 1:225065/1‑90 (MQ=255) ttATGTGGGGCATTGTCGGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCa > 1:320721/1‑90 (MQ=255) aTGTGGGGCATTGTCGGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCaaa > 2:350647/1‑90 (MQ=255) ggcGGGGGGTGGAGGGGGGGTGGTGTCGCTTTACCCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTcgccgc < 2:415080/88‑1 (MQ=255) gCTGATGGGGGCTGGTTTCGCATTACCCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGa < 1:349603/90‑1 (MQ=255) tGATGGGGGTGGGTGTCGCATTACCCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAag < 2:75587/90‑1 (MQ=255) cTGGTGTCGCATTACCCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGAt < 1:87136/90‑1 (MQ=255) aaCCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTactga > 2:292807/1‑90 (MQ=255) | CGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGTCGGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTACTGA > NZ_CP009273/3963341‑3963505 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |