| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 4,527,926 | 0 | A | C | 53.8% | 6.9 / 15.2 | 13 | G219G (GGT→GGG) | nanM | N‑acetylneuraminate epimerase |
| Reads supporting (aligned to +/- strand): ref base A (4/2); new base C (0/7); total (4/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.10e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.56e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
AATTATTACCGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAATCCTGGTTTGGCTTCGCCATTAATAAGCCAGGTTTTATCACCTTTATT‑CACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAG > NZ_CP009273/4527841‑4528001 | aaTTATTACCGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAATCCTGGTTTGGCTTCGCCATTAATAAGCCAGGTTTTATCACCtt < 1:149046/90‑1 (MQ=255)aaTTATTACCGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAATCCTGGTTTGGCTTCGCCATTAATAAGCCAGGTTTTATCACCtt < 1:474636/90‑1 (MQ=255) attaCCGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAATCCTGGTTTGGCTTCGCCATTAATAAGCCAGGTTTTATCACCTTTAtt > 1:106678/1‑90 (MQ=255) tGAAATCAAGTTCAAATACGGCATCCGTTCGCAATCCTGGTTTGGCTTCGCCATTAATAAGCCAGGTTTTATCACCTTTATT‑CACAAccg > 2:596239/1‑90 (MQ=255) aaaTCAAGTTCAAATACGGCATCCGTTCGCAATCCTGGTTTGGCTTCGCCATTAATAAGCCAGGTTTTATCACCTTTATT‑CACAAccgcc > 1:31882/1‑90 (MQ=255) aaaTAAAGTCAAAATACGGCATCCGTTCACAACCCTGGTTTGGCTGCCCCATTAATAAGCAAGGTTTTATCCCCTTTATC‑CACAAccgcc < 1:465129/90‑1 (MQ=255) aatttCAAATCCGGCACCCGTTCGCAATCCTGGTTTGGCTTCGCCATTAAAAAGCCAGGTTTTATCCCCTTTATC‑CACAACCGCCGCAcc < 1:170568/87‑1 (MQ=255) atccGGCATCCGTTCCCACCCCTGTTTGGGCTCCCCATTTAATAGGCAATTTTTTATACCTTTTATT‑CACAACCGCCGCACCAGCCGTTc < 1:417340/87‑1 (MQ=255) cGGCATCCGTTCGCAATCCTGGTTTGGCTTCGCCATTAATAAGCCAGGTTTTATCACCTTTATT‑CACAACCGCCGCACCAGCCGTTCCGt > 2:187376/1‑90 (MQ=255) tCCTGTTTTGGCTTCCCAATAAAAAAGCCGGTTTTTACCCCTTTTTTC‑CACACCCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGcc < 1:453168/90‑1 (MQ=255) caaTTAAAAAGCCGGTTTTTTCCCCCTTTATC‑CACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGc < 1:98192/88‑1 (MQ=255) ttAAAAAACCCGGTTTTTACCCCTTTTTTACAAACCCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCtg < 1:187376/90‑1 (MQ=255) ccccAGTTTTTATCCCCTTTACC‑CACACCCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAg < 1:182769/88‑1 (MQ=255) | AATTATTACCGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAATCCTGGTTTGGCTTCGCCATTAATAAGCCAGGTTTTATCACCTTTATT‑CACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAG > NZ_CP009273/4527841‑4528001 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |