| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 822,955 | 0 | T | G | 55.6% | 16.9 / 29.9 | 27 | T495P (ACC→CCC) | ybhF | ATP‑binding cassette domain‑containing protein |
| Reads supporting (aligned to +/- strand): ref base T (4/8); new base G (15/0); total (19/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.23e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.53e-04 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TCATCCATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAACAGGCCAGCGCCAGCCGCTGTT > NZ_CP009273/822873‑823041 | tCATCCATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGgggggggggg > 2:1342690/1‑90 (MQ=255)tCATCCATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAgg < 1:1190414/87‑1 (MQ=255)tCATCCATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAgg > 2:1190414/1‑87 (MQ=255) aTCCATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGgggggggggggg > 1:295918/1‑88 (MQ=255) gggtggTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGGGGGGGGGGACGCCGGGAg > 2:869605/1‑90 (MQ=255) gggtggTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGAGGGGGGCAACGCCGGAAg > 2:875904/1‑90 (MQ=255) gACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGGGGGGGGGAACGCCGGAAGGCGGGtc > 1:1222461/1‑90 (MQ=255) gACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGGGGGGGGCAACGCCGGAAGGCgggtt > 2:1357825/1‑87 (MQ=255) aTCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTtcgtcg < 2:1277825/90‑1 (MQ=255) aTCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGGGGGGGGCAAAGCCGGGAGGCGGGtcgtcg > 2:724465/1‑90 (MQ=255) tCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGGGGGGGGCAAAGCCGGGAGTCGGGTCGGCga > 2:259906/1‑90 (MQ=255) gTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGGGGGGGGGCACGCCGGGAGGCGGGTCGTCGAGaaaa > 1:335539/1‑89 (MQ=255) gTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGGGGGGGGCAACGCCGGGAGGCGGGTCGTCGAGaaaa > 2:265104/1‑89 (MQ=255) ctACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGtt < 2:1048265/90‑1 (MQ=255) aTGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGGGGGGGGGAACGCCGGGAGGCGGGTGGTCGGGAAACAGAAAGTCCGGGTCCTGc > 1:943128/1‑90 (MQ=255) aTGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGc < 2:1222461/90‑1 (MQ=255) aTGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGGTCATGc > 2:546435/1‑90 (MQ=255) aTGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGGGGGGGGCAACGCCGGGAGGCGGTTCGTCGAGAAAAAGAATGTCCGGGTCATGc > 1:1048265/1‑90 (MQ=255) aTGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGAGGGGGGCAACGCCGGAAGTCGGGTCGTCGAGAAAAAAAAAGTCCCGGTCATGc > 2:1178907/1‑90 (MQ=255) aTGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGAGGGGGGCAAAGCCGGGAGGCGGTTTGTCGAGAAAAAGAAAGTCCGGGTCATGc > 2:1044166/1‑90 (MQ=255) tGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGGGGGGGGCAACGCCGGGAGGCGGGTTGTCGGGAAAAAGAAAGGGCGGGTCATGCa > 1:1369341/1‑90 (MQ=255) tGATGTGCAGCCAAAATTCACGGCGGGGGGGGGGGGGAACGCCGGAAGGCGGGTCGGCGGGAAAAAGAAAGGTCGGGTTATGCATCAGCg > 1:1149549/1‑90 (MQ=255) tgtgCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAAc < 1:300820/90‑1 (MQ=255) tgtgCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAAc < 1:21118/90‑1 (MQ=255) tgtgCAGCCAAAATTCACGGCGGGGGGGGGGGGCAACGCCGGGAGTCGGGTCGTCGGGAAAAAAAAAGTCCGGGTCATGCATCAGCGAAc > 2:1229061/1‑90 (MQ=255) gtgCAGCCAAAATTCACGGCGGGTGAGGGGGGCAACCCCGGAAGTCGGGTCGTCGGGAAAAAGAATGTCCGGGTCATGCATCAGCGaaga > 2:499423/1‑88 (MQ=255) gCAGCCAAAATTCACGGCGGGGGGGGGGGGGAACGCCGGGAGGCGGGTCGTCGAGAAAAAAAAAGTTCGGTTCATGCATCAGCGAACAgg > 1:1318443/1‑90 (MQ=255) cggcggGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAACAGGCCAGCGCCAGCCGc < 2:1139619/90‑1 (MQ=255) cggcggGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAACAGGCCAGCGCCAGCCGc < 1:265104/90‑1 (MQ=255) ggggGAGGGGGGCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGGTCATGCATCAGCGAACAGGCCCGCGCCAGCCGCCGtt > 2:321655/1‑90 (MQ=255) | TCATCCATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAACAGGCCAGCGCCAGCCGCTGTT > NZ_CP009273/822873‑823041 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |