| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 982,447 | 0 | A | C | 61.5% | 8.9 / 11.9 | 13 | intergenic (‑9/+594) | ompF/asnS | porin OmpF/asparagine‑‑tRNA ligase |
| Reads supporting (aligned to +/- strand): ref base A (5/0); new base C (0/8); total (5/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.77e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.13e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TTTCTGCAGCGTTTGCAGTACCTGCTACTAACAGAGCAGGGACGATCACTGCCAGAATATTGCGCTTCATCATTATTTATTACCCTCATGGTTTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGG > NZ_CP009273/982366‑982533 | tttCTGCAGCGTTTGCAGTACCTGCTACTAACAGAGCAGGGACGATCACTGCCAGAATATTGCGCTTCATCATTATTTATTACCCTCATg > 1:316256/1‑90 (MQ=255)tttCTGCAGCGTTTGCAGTACCTGCTACTAACAGAGCAGGGACGATCACTGCCAGAATATTGCGCTTCATCATTATTTATTACCCTCATg > 1:650576/1‑90 (MQ=255) gCAGCGTTTGCAGTACCTGCTACTAACAGAGCAGGGACGATCACTGCCAGAATATTGCGCTTCATCATTATTTATTACCCTCATGGtttt > 1:927585/1‑90 (MQ=255) gATCACTGCCAGAATATTGCGCTTCATCATTATTTATTACCCTCATGGTTTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTc > 1:947043/1‑90 (MQ=255) ccTGCCAAAATTTTGCGCTTCATCATTTTTTATTCCCCTCATGGTTTTTTTTATGACCCCTGCCACTGCCGTCAATAAGTTCTGTCAATa < 2:650576/89‑1 (MQ=255) tGCCAGAATATTGCGCTTCATCATTATTTATTACCCTCATGGTTTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATaaa > 1:284161/1‑90 (MQ=255) atatTGCGCTTCATCATTTTTTATTCCCCTCATGTTTTTTTTTATGACTCCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTAc < 1:1340543/90‑1 (MQ=255) atatTGCGCTTCATCATTATTTATTCCCCTCATGGTTTTTTTTATGACCCCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTAc < 2:745324/90‑1 (MQ=255) ttGCGCTTCATCATTATTTATTCCCCTCATGGTTTTTTTTATGACCCCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGa < 2:1324662/90‑1 (MQ=255) gcgcTTCATCATTATTTTTTCCCCTCATGTTTTTTTTTATGACCCCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAAc < 1:292267/90‑1 (MQ=255) gcccTTCACCATTATTTATTCCCCCCAGGTTTTTTTTTATGGCCCCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAAc < 1:636749/87‑1 (MQ=255) tcatTATTTATTCCCCTCATGTTTTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATtgatg < 1:29007/90‑1 (MQ=255) tcatTATTTATTCCCCTCATGGTTTTTTTTATGACCCCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATtgatg < 2:898190/90‑1 (MQ=255) attaCCCTCATGGTTTTTTTTATGACCCCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTgg < 2:927585/90‑1 (MQ=255) | TTTCTGCAGCGTTTGCAGTACCTGCTACTAACAGAGCAGGGACGATCACTGCCAGAATATTGCGCTTCATCATTATTTATTACCCTCATGGTTTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGG > NZ_CP009273/982366‑982533 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |