| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,213,870 | 0 | A | C | 52.6% | 24.0 / 25.9 | 19 | T363P (ACC→CCC) | pdeG | cyclic diguanylate phosphodiesterase |
| Reads supporting (aligned to +/- strand): ref base A (7/2); new base C (0/10); total (7/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.14e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.68e-05 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TCATATTGGAATTAATTTTAGCGCTTCGCATATTATTTCGCCGACGTTTGTCGACGAGTGTTTAAATTTCCGTGACAGTTTTACCCGCCGCGATTTAAACCTTGTTCTGGAAGTCACCGAGCGTGAGCCATTGAATGTTGATGAAAGTCTGGTTCAGCGG > NZ_CP009273/1213788‑1213947 | tCATATTGGAATTATTTTTAGCGCTTCGCTTTTTTTTTCCCCGACTTTTGTCGACGAGTTTTTAATTTTCCGTGACAGTTTTACccgccg < 2:323982/90‑1 (MQ=255) tGGAATTAATTTTAGCGCTTCGCATATTATTTCGCCGACGTTTGTCGACGAGTGTTTAAATTTCCGTGACAGTTTTACCCGCCGCGAttt > 2:862888/1‑90 (MQ=255) tGGAATTAATTTTAGCGCTTCGCATATTATTTCGCCGACGTTTGTCGACGAGTGTTTAAATTTCCGTGACAGTTTTACCCGCCGCGAttt > 2:1106636/1‑90 (MQ=255) taattTTACCGCTTCGAATTTTTTTTCGCCGACTTTTGTCGACGAGTTTTTAATTTTCCGTGACATTTTTCCCCGCCGCGATTTAAACCt < 1:338347/90‑1 (MQ=255) aattTTAGCGCTTCGAATTTTTTTCCGCCGACGTTTGTCGAGGAGTTTTTAATTTTCCGTGACATTTTTCCCCGCCGCGATTTAAACCtt < 2:628746/90‑1 (MQ=255) tAGCGCTTCGCATATTATTTCGCCGACGTTTGTCGACGAGTGTTTAAATTTCCGTGACAGTTTTACCCGCCGCGATTTAAACCTTGTTCt > 2:1164244/1‑90 (MQ=255) atatTTTTTCGCCGACGTTTGTCGAGGAGTTTTTAAATTTCCGTGACAGTTTTCCCCGCCGCGATTTAAACCTTGTTCTGGAAGTCACCg < 2:545101/90‑1 (MQ=255) tattTCGCCGACGTTTGTCGACGAGTGTTTAAATTTCCGTGACAGTTTTACCCGCCGCGATTTAAACCTTGTTCTGGAAGTCACCGAGCg > 1:759305/1‑90 (MQ=255) tccgccgcCTTTTGTCGACGAGTTTTTAAATTCCCGGGACATTTTTCCCCGCCGCGATTTAAACCTTGTTCTGGAAGTCACCGAGCGTGa < 2:1149787/88‑1 (MQ=255) cgccgcCTTTTGTCGACGATTTTTTAATTTTCCGTGACATTTTTCCCCGCCGCGATTTAAACCTTGTTCTGGAAGTCACCGAGCGTGAGc < 1:29892/90‑1 (MQ=255) cGCCGAGGTTTGTCGACGAGTTTTTAAATTTCCGTGACATTTTTCCCCGCCGCGATTTAAACCTTGTTCTGGAAGTCACCGAGCGTGAGc < 2:1186724/90‑1 (MQ=255) cGCCGACGTTTGTCGACGAGTGTTTAAATTTCCGTGACAGTTTTACCCGCCGCGATTTAAACCTTGTTCTGGAAGTCACCGAGCGTGAGc > 1:1090789/1‑90 (MQ=255) cGCCGACGTTTGTCGACGAGTGTTTAAATTTCCGTGACAGTTTTACCCGCCGCGATTTAAACCTTGTTCTGGAAGTCACCGAGCGTGAGc > 1:582721/1‑90 (MQ=255) ttAATTTCCCGTAACTTTTTTCCCCGCCGCGATTTAAACCTTGTTCTGGAAGTCACCGAGCGTGAGCCATTGAATGTTGATGAAAGTCTg < 1:1240327/90‑1 (MQ=255) ttCCGTGACAGTTTTACCCGCCGCGATTTAAACCTTGTTCTGGAAGTCACCGAGCGTGAGc > 1:724586/1‑61 (MQ=255) ttCCGTGACAGTTTTACCCGCCGCGATTTAAACCTTGTTCTGGAAGTCACCGAGCGTGAGc < 2:724586/61‑1 (MQ=255) tCCGTGACAGTTTTCCCCGCCGCGATTTAAACCTTGTTCTGGAAGTCACCGAGCGTGAGCCATTGAATGTTGATGAAAGTCTGGTTCAGc < 2:308434/90‑1 (MQ=255) cccGTGACATTTTTCCCCGCCGCGATTTAAACCTTGTTCTGGAAGTCACCGAGCGTGAGCCATTGAATGTTGATGAAAGTCTGGTTCAGc < 2:759305/89‑1 (MQ=255) cGTGACATTTTTCCCCGCCGCGATTTAAACCTTGTTCTGGAAGTCACCGAGCGTGAGCCATTGAATGTTGATGAAAGTCTGGTTCAGCgg < 2:1106433/90‑1 (MQ=255) | TCATATTGGAATTAATTTTAGCGCTTCGCATATTATTTCGCCGACGTTTGTCGACGAGTGTTTAAATTTCCGTGACAGTTTTACCCGCCGCGATTTAAACCTTGTTCTGGAAGTCACCGAGCGTGAGCCATTGAATGTTGATGAAAGTCTGGTTCAGCGG > NZ_CP009273/1213788‑1213947 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |