| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,316,700 | 0 | A | T | 53.3% | 9.1 / 20.7 | 15 | N168K (AAT→AAA) | trpE | anthranilate synthase component I |
| Reads supporting (aligned to +/- strand): ref base A (6/1); new base T (0/8); total (6/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.40e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.72e-04 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
AACAGGCTGGCCTGAATACGGGTGCTTTTTTTCTGATGGTCAATCACCATCAGCGTTTCAGCGAGATAAAAACAGAAATCAGGGCAGTTATTTTCCGCTGACAGTTGCGGTAAATCTTCAAATCCCGCCACAAGGTCATAAGAGAACAGGCCGCCGA > NZ_CP009273/1316611‑1316767 | aaCAGGCTGGCCTGAATACGGGTGCTTTTTTTCTGATGGTCAATCACCATCAGCGTTTCAGCGAGATAAAAACAGAAATCAGGGCAGTTa > 1:893946/1‑90 (MQ=255) gCTTTTTTTCTGATGGTCAATCACCATCAGCGTTTCAGCGAGATAAAAACAGAAATCAGGGCAGTTATTTTCCGCTGACAGTTGCGGTaa > 1:962/1‑90 (MQ=255) gCTTTTTTTCTGATGGTCAATCACCATCAGCGTTTCAGCGAGATAAAAACAGAAATCAGGGCAGTTATTTTCCGCTGACAGTTGCGGTaa > 2:936400/1‑90 (MQ=255) tCTGATGGTCAATCACCATCAGCGTTTCAGCGAGATAAAAACAGAAATCAGGGCAGTTATTTTCCGCTGACAGTTGCGGTAAATCTTCaa < 2:239409/90‑1 (MQ=255) cATCAGCTTTTCAGCGAAAAAAAAAAAAAAATCGGGGAAGTTTTTTTCCGGGGACTGTTGGGGTAAATTTAAAACCCCCGCCACAAGGTc < 1:1238148/90‑1 (MQ=255) cATCAGCTTTTCAGCAAAAAAAAAAAAAAAAACAGGGCATTTTTTTTCCGCTGACAGTTGCGAAAAATCTTAAAACCCCGCCACAAGGTc < 1:1276594/90‑1 (MQ=255) cATCAGCTTTCCAGCGAGATAAAAACAAAAATCGGGGCATTTTTTTCCCGCTGACAGTTGCGGTAAATCTTCAAACCCCGCCACAAGGTc < 1:215447/90‑1 (MQ=255) cATCAGCGTTTCAGCGAGATAAAAACAGAAATCAGGGCAGTTATTTTCCGCTGACAGTTGCGGTAAATCTTCAAATCCCGCCACAAGGTc > 2:1276594/1‑90 (MQ=255) cATCAGCGTTTCAGCGAGAAAAAAACAAAAATCAGGGCAGTTTTTTTCCGCTGACAGTTGCGGAAAATCTTCAAATCCCGCCACAAGGTc < 2:452522/90‑1 (MQ=255) aTCAGCGTTTCAGCGAGATAAAAACAGAAATCAGGGCAGTTATTTTCCGCTGACAGTTGCGGTAAATCTTCAAATCCCGCCACAAGGTCa > 1:452522/1‑90 (MQ=255) aTCAGCGTTTCAGCGAGATAAAAACAGAAATCAGGGCAGTTATTTTCCGCTGACAGTTGCGGTAAATCTTCAAATCCCGCCACAAGGTCa > 1:592740/1‑90 (MQ=255) tCAGCTTTTCAGAGAAAAAAAAACAGAAATCAGGGCATTTTTTTCCCGCTGACAGTTGGGGAAAATCTTAAAATCCCGCCACAAGGTCAt < 2:666079/90‑1 (MQ=255) cAGCGTTTCAGCGAGATAAAAACAGAAATCAGGGCAGTTATTTTCCGCTGACAGTTGCGGTAAATCTTCAAATCCCGCCACAAGGTCATa > 1:136486/1‑90 (MQ=255) cgCGTTTCAGCGAAAAAAAAACAAAAATCAGGGCAGTTTTTTCCCGCTGACAGTTGCGGTAAATCTTAAAATCCCGCCACAAGGTCATaa < 2:962/89‑1 (MQ=255) tCAGCGAAAAAAAAACAAAAACCGGGGAATTTTTTTCCCGCTGACAGTGGGGGAAAATCTTCAACCCCCGCCACAAGGTCATAAGAGAAc < 1:936400/90‑1 (MQ=255) aaaaaaaaaaACCAGGAAATTTTTTTCCCGCGGACAGTGGGGGAAAACCTAAAAACCCCGCCACAAGGTCATAAGAGAACAGGCCGCCga < 1:460289/90‑1 (MQ=255) | AACAGGCTGGCCTGAATACGGGTGCTTTTTTTCTGATGGTCAATCACCATCAGCGTTTCAGCGAGATAAAAACAGAAATCAGGGCAGTTATTTTCCGCTGACAGTTGCGGTAAATCTTCAAATCCCGCCACAAGGTCATAAGAGAACAGGCCGCCGA > NZ_CP009273/1316611‑1316767 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |