| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,663,765 | 0 | T | A | 57.1% | 6.3 / 16.8 | 14 | I29F (ATT→TTT) | ynfL | LysR family transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base T (0/6); new base A (8/0); total (8/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.33e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.05e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
ATACACTGCGATTGGTTCGTGCCAGCAGTCGGGCACCAATTTGTTGCTCCAGCGCCTGAATCTGCTGACTTAGCGGCGGTTGCGAAATATTCAGGCGGGCAGCGGCGCGCCCGAAATGCAGCTCTTCCGCAACAGCAACAAAGTAACGCAGATGACGAAGTTCAAT > NZ_CP009273/1663678‑1663843 | aTACACTGCGATTGGTTCGTGCCAGCAGTCGGGCACCAATTTGTTGCTCCAGCGCCTGAATCTGCTGACTTAGCGGCGGGTGCGGAaaat > 2:1093967/1‑90 (MQ=255) cTGCGATTGGTTCGTGCCAGCAGTCGGGCACCAATTTGTTGCTCCAGCGCCTGAATCTGCTGACTTAGCGGCGGGTGGGGAAAATTTAgg > 1:192141/1‑90 (MQ=255) cTGCGATTGGTTCGTGCCAGCAGTCGGGCACCAATTTGTTGCTCCAGCGCCTGAATCTGCTGACTTAGCGGCGGGTGCGGAAAAtttaag > 2:1373810/1‑86 (MQ=255) cTGCGATTGGTTCGTGCCAGCAGTCGGGCACCAATTTGTTGCTCCAGCGCCTGAATCTGCTGACTTAGCGGCGGGTGCGAAAAATTCAgg > 2:182020/1‑90 (MQ=255) gCGATTGGTTCGTGCCAGCAGTCGGGCACCAATTTGTTGCTCCAGCGCCTGAATCTGCTGACTTAGCGGCGGTTGCGAAAAATTCAGGCg > 2:41683/1‑90 (MQ=255) ggTTCGTGCCAGCAGTCGGGCACCAATTTGTTGCTCCAGCGCCTGAATCTGCTGACTTAGCGGCGGTTGCGAAAAATTTAgggggggggg > 2:919789/1‑86 (MQ=255) cagcagTCGGGCACCAATTTGTTGCTCCAGCGCCTGAATCTGCTGACTTAGCGGCGGTTGCGAAATATTCAGGCGGGCAGCGGCGCGccc < 2:504192/90‑1 (MQ=255) gcagTCGGGCACCAATTTGTTGCTCCAGCGCCTGAATCTGCTGACTTAGCGGCGGGTGCGGAAAATTTCGGCGGGGCGGGGGGCGccccc > 1:541853/1‑88 (MQ=255) agaaTTTGTTGCTCCAGCGCCTGAATCTGCTGACTTAGCGGCGGTTGCGAAATATTCAGGCGGGCAGCGGCGCGCCCGAAATGCAGctct < 1:424939/88‑1 (MQ=255) tCCAGCGCCTGAATCTGCTGACTTAGCGGCGGTTGCGAAAAATTTAGGGGGGGAGCGGCGCGCCCCAAAAGCAGCTCTTCCgccacagct > 2:424939/1‑89 (MQ=255) ccTGAATCTGCTGACTTAGCGGCGGTTGCGAAATATTCAGGCGGGCAGCGGCGCGCCCGAAATGCAGCTCTTCCGCAACAGCAACAAAGt < 1:919789/90‑1 (MQ=255) tGAATCTGCTGACTTAGCGGCGGTTGGGAAAAATTTAGGCGGGCCGGGGGGCGCCCCGAAAACAGCCCTTCCCCCACAACaaaaaaaaaa > 2:679696/1‑86 (MQ=255) cggcggTTGCGAAATATTCAGGCGGGCAGCGGCGCGCCCGAAATGCAGCTCTTCCGCAACAGCAACAAAGTAACGCAGATGACGAAGTTc < 1:41683/90‑1 (MQ=255) gcggTTGCGAAATATTCAGGCGGGCAGCGGCGCGCCCGAAATGCAGCTCTTCCGCAACAGCAACAAAGTAACGCAGATGACGAAGTTCaa < 1:679696/90‑1 (MQ=255) cggTTGCGAAATATTCAGGCGGGCAGCGGCGCGCCCGAAATGCAGCTCTTCCGCAACAGCAACAAAGTAACGCAGATGACGAAGTTCAat < 1:177479/90‑1 (MQ=255) | ATACACTGCGATTGGTTCGTGCCAGCAGTCGGGCACCAATTTGTTGCTCCAGCGCCTGAATCTGCTGACTTAGCGGCGGTTGCGAAATATTCAGGCGGGCAGCGGCGCGCCCGAAATGCAGCTCTTCCGCAACAGCAACAAAGTAACGCAGATGACGAAGTTCAAT > NZ_CP009273/1663678‑1663843 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |