| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,370,630 | 0 | A | C | 57.1% | 5.5 / 10.8 | 14 | Y148D (TAT→GAT) | menH | 2‑succinyl‑6‑hydroxy‑2, 4‑cyclohexadiene‑1‑carboxylate synthase |
| Reads supporting (aligned to +/- strand): ref base A (4/2); new base C (0/8); total (4/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.50e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.05e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GGCAAGCGTTGCGCCATTATTGTTGCTGCGCAGCGCCACCAGCTCCCGGCGTTGATCGTCATTGAGTGAGGCAAAAACAGGCTGTTGATACCAGTCGGCAAATACCGCCGTTAACGGTTCTGTGAGAAAACGCTGCACCCATTGGCGATCGGAACGCTGACGTTCCGCACGTTGTTCAG > NZ_CP009273/2370541‑2370719 | ggcAAGCGTTGCGCCATTATTGTTGCTGCGCAGCGCCACCAGCTCCCGGCGTTGATCGTCATTGAGTGAGGCAAAAACAGGCTGTTGATa > 2:296842/1‑90 (MQ=255) ttttgttgCTGCGCAGCGCCCCCAGCCCCCGGCGTTGATCGTCATTGAGTGAGGCAAAAACAGGCTGTTGATACCAGTCGGCAAATAccg < 2:981104/88‑1 (MQ=255) tgctgcGCAGCGCCACCAGCTCCCGGCGTTGATCGTCATTGAGTGAGGCAAAAACAGGCTGTTGATACCAGTCGGCAAATACCGCCGTTa > 1:1187566/1‑90 (MQ=255) tgctgcGCAGCGCCACCAGCTCCCGGCGTTGATCGTCATTGAGTGAGGCAAAAACAGGCTGTTGATACCAGTCGGCAAATACCGCCGTTa > 2:84576/1‑90 (MQ=255) ctgcGCAGCGCCACCAGCTCCCGGCGTTGATCGTCATTGAGTGAGGCAAAAACAGGCTGTTGATCCCAGTCGGCAAATACCGCCGTTAAc < 2:1092576/90‑1 (MQ=255) cgcCCCCAGCTCCCGGCGTTGATCGTCATTGAGGGAGGAAAAAACGGGCTTTTGACCCCAGTCGGAAAATACCGCCGTTAACGGTTCtgt < 1:1250058/90‑1 (MQ=255) ccccaccaGCCCCCGGCGTTGATCGTCATTGAGTGAGGAAAAAACAGGCTGTTGATCCCAGTCGGCGAATACCGCCGTTAACGGTTCtgt < 2:1167237/88‑1 (MQ=255) ccaGCCCCCGGGGTTGATCGTCATGGAGGGAGGAAAAAACGGGCTGTTGATCCCAGTCGGAAAATACCGCCGTTAACGGTTCTGTGAGaa < 1:1233447/90‑1 (MQ=255) cTCCCGGCGTTGATCGTCATTGAGTGAGGCAAAAACAGGCTGTTGATACCAGTCGGCAAATACCGCCGTTAACGGTTCTGTGAGAAAACg > 1:894435/1‑90 (MQ=255) ccGGCGTTGATCGTAATGGAGTGAGGAAAAAACGGGCTGTTGATACCAGTCGGAAAATACCGCCGTTAACGGTTCTGTGAGAAAACGCTg < 1:1136276/90‑1 (MQ=255) aTCGTCATTGAGTGAGGCAAAAACAGGCTGTTGATCCCAGTCGGCAAATACCGCCGTTAACGGTTCTGTGAGAAAACGCTGCACCCATTg < 2:1341011/90‑1 (MQ=255) agGCAAAAACAGGCTGTTGATACCAGTCGGCAAATACCGCCGTTAACGGTTCTGTGAGAAAACGCTGCACCCATTGGCGATCGGAACGCt > 1:917383/1‑90 (MQ=255) ggaaaaaaCAGGCTGTTGATCCCAGTCGGAAAATACCGCCGTTAACGGTTCTGTGAGAAAACGCTGCACCCATTGGCGATCGGAACGCTg < 2:1187566/87‑1 (MQ=255) aaaaaaaCGGGTTTTTAATCCCAGCCGGAAAATCCCGCCGTTAACGGTTCTGTGAGAAAACGCTGCACCCATTGGCGATCGGAACGCTGa < 1:296842/88‑1 (MQ=255) ttttGATCCCAGTCGGAAAATCCCGCCGTTAACGGTTCTGTGAGAAAACGCTGCACCCATTGGCGATCGGAACGCTGACGTTCCGCACgt < 2:1176932/88‑1 (MQ=255) aCCAGTCGGCAAATACCGCCGTTAACGGTTCTGTGAGAAAACGCTGCACCCATTGGCGATCGGAACGCTGACGTTCCGCACGTTGTTCAg > 1:1112452/1‑90 (MQ=255) | GGCAAGCGTTGCGCCATTATTGTTGCTGCGCAGCGCCACCAGCTCCCGGCGTTGATCGTCATTGAGTGAGGCAAAAACAGGCTGTTGATACCAGTCGGCAAATACCGCCGTTAACGGTTCTGTGAGAAAACGCTGCACCCATTGGCGATCGGAACGCTGACGTTCCGCACGTTGTTCAG > NZ_CP009273/2370541‑2370719 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |