| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 822,963 | 0 | T | G | 56.5% | 13.3 / 30.5 | 23 | D492A (GAC→GCC) | ybhF | ATP‑binding cassette domain‑containing protein |
| Reads supporting (aligned to +/- strand): ref base T (0/10); new base G (13/0); total (13/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.74e-07 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.23e-05 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAACAGGCCAGCGCCAGCCGCTGTTTAAAACCTAAT > NZ_CP009273/822886‑823052 | gggtggTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGGGGGGGGGAACGCCGGAAg > 1:119628/1‑90 (MQ=255)gggtggTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGAGGGGGGGAAAGCCGGAAg > 2:168512/1‑90 (MQ=255) cGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGaaa < 2:387818/90‑1 (MQ=255) acagtttCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGaa < 1:892043/86‑1 (MQ=255) gCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGGGGGGGGCAACGCCCGGAGGCGGGTCGGCGGGAAAAAGaaa > 1:532791/1‑89 (MQ=255) ttCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTcc < 2:908260/90‑1 (MQ=255) ctACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGtt < 2:140918/90‑1 (MQ=255) ccATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCAt < 2:166793/90‑1 (MQ=255) aTGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGGGGGGGGCAACGCCGGAAGGCGGGTCGTCGGGAAACAGAAGGTCCGGTTCAttc > 2:331609/1‑88 (MQ=255) aTGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGGGGGGGGCAACGCCGGAAGTCGGTTCGTCGGGAAAAAGAAAGTCCGGGTCAttc > 2:681699/1‑88 (MQ=255) aTGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGGGGGGGGCAAAGCCGGGAGTCGGGTCGTCGAGAAACAGAAAGTCCGGGTCATGc > 2:62093/1‑90 (MQ=255) aTGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGAGGGGGGCAACGCCGGGAGGCGGGTTGTCGAGAAAAAAAAAGTCCGGTTCATGc > 2:782977/1‑90 (MQ=255) gCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGGGGGGGGGAACGCCGGGAGTCGGGTTGTCGAGAAAAAAAAAGTCCGGGTCATGCAt > 1:363647/1‑90 (MQ=255) gCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGGGGGGGGGAACGCCGGGAGAGGGGTCGTCGGGGAAAAAAAAGGCCGGGTGATGCAt > 1:934318/1‑90 (MQ=255) gTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAg < 2:227212/90‑1 (MQ=255) gTTGATGTGCAGCCAAAATTCACGGCGGGGGGGGGGGGGAACGCCGGGAGTCGGTTCGTCGTGAAAAAAAAAGTCCGGGTTATGCATTAg > 2:791128/1‑90 (MQ=255) tgtgCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAAc < 1:119388/90‑1 (MQ=255) tgtgCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAAc < 1:159176/90‑1 (MQ=255) gCAGCCAAAATTCACGGCGGGGGGGGGGGGGAACGCCGGAAGGCGGTTCGTCGAGAAAAAGAATGTCCGGGTCATGCATCAGCGAACAgg > 2:666908/1‑90 (MQ=255) gCAGCCAAAATTCACGGCGGGGGAGGGGGGCAACGCCGGAAGGCGGTTCGTCGAGAAAAAGAATGTCCGGGTCATGCATCAGCGAACAgg > 1:54531/1‑90 (MQ=255) aGCCAAAATTCACGGCGGGGGGGGGGGGCAACGCCGGGAGTCGGGTCGTCGGGAAAAAGAAAGTCCGGGTCATGCATCAGCGAACAgggc > 1:53115/1‑88 (MQ=255) cggcggGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAACAGGCCAGCGCCAGCCGc < 1:344563/90‑1 (MQ=255) cggcggGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAACAGGCCAGCGCCAGCCGc < 1:791128/90‑1 (MQ=255) ggggCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGGTCATGCATCAGCGAACAGGCCAGCGCCAGCCGCTGGTTAAAACCt > 1:827980/1‑90 (MQ=255) tCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAACAGGCCAGCGCCAGCCGCTGTTTAAAACCTAAt > 2:406586/1‑90 (MQ=255) | GGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAACAGGCCAGCGCCAGCCGCTGTTTAAAACCTAAT > NZ_CP009273/822886‑823052 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |