| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 832,042 | 0 | C | G | 60.0% | 0.4 / 18.4 | 20 | A79G (GCG→GGG) | hcxB | hydroxycarboxylate dehydrogenase HcXB |
| Reads supporting (aligned to +/- strand): ref base C (4/4); new base G (12/0); total (16/4) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.44e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.35e-02 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GATCCCAAGCTATGTACGCTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGTCACGCTCGATGGCGATCGCGCATTTGGTCAGGTCGCGGCACATGAAGCGATGGCGCTGGGGATTGAGAAAGCGCATCAGCACG > NZ_CP009273/831962‑832131 | gATCCCAAGCTATGTACGCTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGgggggggggggccc > 1:869526/1‑86 (MQ=255)gATCCCAAGCTATGTACGCTCCTGGAGTCAGAGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGGGGGGGGCAc > 1:181807/1‑90 (MQ=255) aTGTACGCTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGGGGGGGGCACGCGCCATggag > 2:396765/1‑88 (MQ=255) aTGTACGCTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGTGACCCCCGAAgggg > 2:473093/1‑88 (MQ=255) cTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGGGGGGGGGACGCTCCCTGGCGAGCccgc > 1:391022/1‑90 (MQ=255) gTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGGGGGGGGCACCCCCCCTGGGGAGCGCGCCTTTTGTc > 1:832192/1‑90 (MQ=255) cAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGGGGGGGGCACGCTCGTTGGGGATCGCGCATTTTGTCAg > 2:44642/1‑90 (MQ=255) cAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGGGGCGGGCCCGCTCGATGGCGATCGCGCATTTGGTCAg > 2:387401/1‑90 (MQ=255) ggggCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGGGGCGGGCCCGCTCGATGGCGATCGCGCATTTGGTCAggg > 2:591755/1‑89 (MQ=255) gCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGGGGCGGGCACCCTCGATGGGGATCGCGCCTTTTGTCAGGgcgc > 2:812590/1‑90 (MQ=255) aCCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGGGGGGGGGACGCCCGAGGGGGAACGCGCCTTTTGGGAGGGCGCgg > 1:298094/1‑90 (MQ=255) tGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGGGGGGGGCACCCCCCATGGCGAGCCCGCCTTTTGGCAGGGCGCGGgac > 1:826285/1‑90 (MQ=255) cAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGGGGCGGGCCCGCCCGATGGCGAACGCGCCTTTTGGCCGGGGGCGGGACAt > 1:47412/1‑90 (MQ=255) aaCCATCATGCCAAAACCGTTAAAGAGGCGGGGGGGGGGGCCCCGCCCCCTGGCGGCCGCGCATTTGGGCAGGGCGCGGCGCAGGAAGCg > 1:285386/1‑90 (MQ=255) catcatGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGGCACGCTCGATGGCGGTCGCGCCTTTGGTCAGGTCGCGGCACATGAAGCGATg > 2:772982/1‑90 (MQ=255) atcatGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGTCACGCTCGATGGCGATCGCGCATTTGGTCAGGTCGCGGCACATGAAGCGATgg < 2:508049/90‑1 (MQ=255) ccAAAACCGTTAAAGAGGCGGGGGCGGCGGTCACGCTCGATGGCGATCGCGCATTTGGTCAGGTCGCGGCACATGAAGCGATGGCGCTgg < 2:645169/90‑1 (MQ=255) ccAAAACCGTTAAAGAGGCGGGGGCGGCGGTCACGCTCGATGGCGATCGCGCATTTGGTCAGGTCGCGGCACATGAAGCGATGGCGCTgg < 1:387401/90‑1 (MQ=255) gTTAAAGAGACGGGGGCGGCGGTCACACTCGATGGCGATCGCGCATTTGATCAGGTCGCGGCACATGAAGCGATGGCGCTGGGGATTgag > 2:363823/1‑90 (MQ=255) ttAAAGAGGCGGGGGCGGCGGGCACGCTCGATGGCGATCGCGCATTTGGTCAGGGCGCGGGACATGAAGCGATGGCGCTGGGGGTTgaga > 2:307864/1‑90 (MQ=255) cGGGGGCGGCGGTCACGCTCGATGGCGATCGCGCATTTGGTCAGGTCGCGGCACATGAAGCGATGGCGCTGGGGATTGAGAAAGCGCATc < 2:391022/90‑1 (MQ=255) gcggcgGTCACGCTCGATGGCGATCGCGCATTTGGTCAGGTCGCGGCACATGAAGCGATGGCGCTGGGGATTGAGAAAGCGCATCAGCAc < 2:869526/90‑1 (MQ=255) cggcggTCACGCTCGATGGCGATCGCGCATTTGGTCAGGTCGCGGCACATGAAGCGATGGCGCTGGGGATTGAGAAAGCGCATCAGCACg < 1:505503/90‑1 (MQ=255) cggcggTCACGCTCGATGGCGATCGCGCATTTGGTCAGGTCGCGGCACATGAAGCGATGGCGCTGGGGATTGAGAAAGCGCATCAGCACg < 1:304093/90‑1 (MQ=255) | GATCCCAAGCTATGTACGCTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGTCACGCTCGATGGCGATCGCGCATTTGGTCAGGTCGCGGCACATGAAGCGATGGCGCTGGGGATTGAGAAAGCGCATCAGCACG > NZ_CP009273/831962‑832131 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |