Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP009273832,0480TG56.5% 17.2 / 27.2 24V81G (GTC→GGC) hcxBhydroxycarboxylate dehydrogenase HcXB
Reads supporting (aligned to +/- strand):  ref base T (3/7);  new base G (13/0);  total (17/7)
Fisher's exact test for biased strand distribution p-value = 4.89e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.72e-04
Rejected as consensus: Frequency below/above cutoff threshold.

GATCCCAAGCTATGTACGCTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGTCACGCTCGATGGCGATCGCGCATTTGGTCAGGTCGCGGCACATGAAGCGATGGCGCTGGGGATTGAGAAAGCGCATCAGCACGGT  >  NZ_CP009273/831962‑832133
                                                                                      |                                                                                     
gATCCCAAGCTATGTACGCTCCTGGAGTCAGAGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGGGGGGGGCAc                                                                                    >  1:181807/1‑90 (MQ=255)
           aTGTACGCTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGGGGGGGGCACGCGCCATggag                                                                         >  2:396765/1‑88 (MQ=255)
           aTGTACGCTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGTGACCCCCGAAgggg                                                                         >  2:473093/1‑88 (MQ=255)
                  cTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGGGGGGGGGACGCTCCCTGGCGAGCccgc                                                                  >  1:391022/1‑90 (MQ=255)
                          gTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGGGGGGGGCACCCCCCCTGGGGAGCGCGCCTTTTGTc                                                          >  1:832192/1‑90 (MQ=255)
                            cAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGGGGGGGGCACGCTCGTTGGGGATCGCGCATTTTGTCAg                                                        >  2:44642/1‑90 (MQ=255)
                            cAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGGGGCGGGCCCGCTCGATGGCGATCGCGCATTTGGTCAg                                                        >  2:387401/1‑90 (MQ=255)
                              ggggCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGGGGCGGGCCCGCTCGATGGCGATCGCGCATTTGGTCAggg                                                      >  2:591755/1‑89 (MQ=255)
                                 gCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGGGGCGGGCACCCTCGATGGGGATCGCGCCTTTTGTCAGGgcgc                                                   >  2:812590/1‑90 (MQ=255)
                                   aCCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGGGGGGGGGACGCCCGAGGGGGAACGCGCCTTTTGGGAGGGCGCgg                                                 >  1:298094/1‑90 (MQ=255)
                                      tGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGGGGGGGGCACCCCCCATGGCGAGCCCGCCTTTTGGCAGGGCGCGGgac                                              >  1:826285/1‑90 (MQ=255)
                                        cAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGGGGCGGGCCCGCCCGATGGCGAACGCGCCTTTTGGCCGGGGGCGGGACAt                                            >  1:47412/1‑90 (MQ=255)
                                              aaCCATCATGCCAAAACCGTTAAAGAGGCGGGGGGGGGGGCCCCGCCCCCTGGCGGCCGCGCATTTGGGCAGGGCGCGGCGCAGGAAGCg                                      >  1:285386/1‑90 (MQ=255)
                                                 catcatGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGGCACGCTCGATGGCGGTCGCGCCTTTGGTCAGGTCGCGGCACATGAAGCGATg                                   >  2:772982/1‑90 (MQ=255)
                                                  atcatGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGTCACGCTCGATGGCGATCGCGCATTTGGTCAGGTCGCGGCACATGAAGCGATgg                                  <  2:508049/90‑1 (MQ=255)
                                                        ccAAAACCGTTAAAGAGGCGGGGGCGGCGGTCACGCTCGATGGCGATCGCGCATTTGGTCAGGTCGCGGCACATGAAGCGATGGCGCTgg                            <  1:387401/90‑1 (MQ=255)
                                                        ccAAAACCGTTAAAGAGGCGGGGGCGGCGGTCACGCTCGATGGCGATCGCGCATTTGGTCAGGTCGCGGCACATGAAGCGATGGCGCTgg                            <  2:645169/90‑1 (MQ=255)
                                                                gTTAAAGAGACGGGGGCGGCGGTCACACTCGATGGCGATCGCGCATTTGATCAGGTCGCGGCACATGAAGCGATGGCGCTGGGGATTgag                    >  2:363823/1‑90 (MQ=255)
                                                                 ttAAAGAGGCGGGGGCGGCGGGCACGCTCGATGGCGATCGCGCATTTGGTCAGGGCGCGGGACATGAAGCGATGGCGCTGGGGGTTgaga                   >  2:307864/1‑90 (MQ=255)
                                                                          cGGGGGCGGCGGTCACGCTCGATGGCGATCGCGCATTTGGTCAGGTCGCGGCACATGAAGCGATGGCGCTGGGGATTGAGAAAGCGCATc          <  2:391022/90‑1 (MQ=255)
                                                                               gcggcgGTCACGCTCGATGGCGATCGCGCATTTGGTCAGGTCGCGGCACATGAAGCGATGGCGCTGGGGATTGAGAAAGCGCATCAGCAc     <  2:869526/90‑1 (MQ=255)
                                                                                cggcggTCACGCTCGATGGCGATCGCGCATTTGGTCAGGTCGCGGCACATGAAGCGATGGCGCTGGGGATTGAGAAAGCGCATCAGCACg    <  1:505503/90‑1 (MQ=255)
                                                                                cggcggTCACGCTCGATGGCGATCGCGCATTTGGTCAGGTCGCGGCACATGAAGCGATGGCGCTGGGGATTGAGAAAGCGCATCAGCACg    <  1:304093/90‑1 (MQ=255)
                                                                                  gcggTCACGCTCGATGGCGATCGCGCATTTGGTCAGGTCGCGGCACATGAAGCGATGGCGCTGGGGATTGAGAAAGCGCATCAGCACGGt  >  2:841973/1‑90 (MQ=255)
                                                                                      |                                                                                     
GATCCCAAGCTATGTACGCTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGTCACGCTCGATGGCGATCGCGCATTTGGTCAGGTCGCGGCACATGAAGCGATGGCGCTGGGGATTGAGAAAGCGCATCAGCACGGT  >  NZ_CP009273/831962‑832133

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑