Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092732,380,3410GA58.3% ‑2.9 / 11.3 12intergenic (+31/‑72)yfbL/yfbMM28 family metallopeptidase/YfbM family protein
Reads supporting (aligned to +/- strand):  ref base G (1/4);  new base A (7/0);  total (8/4)
Fisher's exact test for biased strand distribution p-value = 1.01e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.93e-03
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

TAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCT  >  NZ_CP009273/2380270‑2380428
                                                                       |                                                                                       
tAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACattttatt                                                                       <  2:429318/90‑1 (MQ=255)
  gTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGGCCCGAAAAAAAGACAAGCACaatttttttt                                                                     >  2:465416/1‑90 (MQ=255)
     gATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTccc                                                                  <  1:915046/90‑1 (MQ=255)
      aTGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCCGAAAAAACGACATGCACAATTTTTTTTTCCt                                                                 >  2:907840/1‑90 (MQ=255)
          tgtTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATc                                                             <  2:789898/90‑1 (MQ=255)
                   tttGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGGCCCGAAAAAAAGACAAGGACATTTTTTTTTTTCCCATCTGTTTTTAg                                                    >  2:468083/1‑90 (MQ=255)
                            caacaGTAAATAATAAAAATTTCTCGAGGCGGGGCCCGAAAAAAAGACAAGCACAATTTTTTTTTTCTCCTCTGTTTTTATATGCGaaaa                                           >  1:694991/1‑89 (MQ=255)
                              acaGTAAATAATAAAAATTTCTCGAGGCGGGGCCAGAAAAAACGACAAGCACATTTTTTTTTTCCTCATCTGTTTTTAGATGCGAAACaa                                         >  2:207522/1‑89 (MQ=255)
                                        ataaAAATTTCTCGAGGCGGGGCCAGAAAAAACGACATGCACATTTTTTTTTTCCTCCTCTGTTTTTAAATGCGAAACAAGAAAATCAAc                               >  2:305015/1‑90 (MQ=255)
                                                ttCTCGAGGCGGGGCCCGAAAAAAAGACAAGCACAATTTTTTTTTCCTCCTCTGTTTTTAAATGAGGAAAAAGAAATTTAAAGGGGGaaa                       >  1:887414/1‑90 (MQ=255)
                                                             tCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAAt          <  2:205071/90‑1 (MQ=255)
                                                                     aaGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCt  >  2:197541/1‑90 (MQ=255)
                                                                       |                                                                                       
TAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCT  >  NZ_CP009273/2380270‑2380428

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑