| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,214,126 | 0 | T | C | 55.0% | 7.5 / 17.8 | 20 | I425T (ATC→ACC) | ygjG | putrescine aminotransferase |
| Reads supporting (aligned to +/- strand): ref base T (5/4); new base C (0/11); total (5/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.13e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.71e-02 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
ATAACGAAATCGGCTATAACTTTGCCAGCGAGATGTTCCGCCAGCGCGTACTGGTGGCCGGAACGCTCAATAACGCCAAAA‑CGATCCGCATTGAACCGCCACTGACACTGACCATTGAACAGTGTGAACTGGTGATCAAAGCGGCGCGTAAGGCGCTGGCGGCCAT > NZ_CP009273/3214042‑3214207 | aTAACGAAATCGGCTATAACTTTGCCAGCGAGATGTTCCGCCAGCGCGTACTGGTGGCCGGAACGCTCAATAACGCCAAAA‑CGATCCGCa > 1:32739/1‑90 (MQ=255) tAACGAAATCGGCTATAACTTTGCCAGCGAGATGTTCCGCCAGCGCGTACTGGTGGCCGGAACGCTCAATAACGCCAAAA‑CGATc < 2:854586/85‑1 (MQ=255) tAACGAAATCGGCTATAACTTTGCCAGCGAGATGTTCCGCCAGCGCGTACTGGTGGCCGGAACGCTCAATAACGCCAAAA‑CGATc > 1:854586/1‑85 (MQ=255) aaaTCGGTTATACTTTTCCCAGGGAGTTTTCCCCCCAGCGCTTCCTGGGGGCGGGAACGCTAAAAACCCCAAAAA‑CGATCCGCATTAAAc < 1:38162/90‑1 (MQ=255) tttGCCAGGGAGTTTTCCCGCCGGCGCGTACTGGGGGCCGGACCGCTAAATAACCCAAAAA‑CGACCCGAATTAAACCGCcactgacactg < 2:251612/90‑1 (MQ=255) ttGCCAGCGAGATGTTCCGCCAGCGCGTACTGGTGGCCGGAACGCTCAATAACGCCAAAA‑CGATCCGCATTGAACCGCcactgacactga > 1:524848/1‑90 (MQ=255) tGCCAGCGAGATGTTC‑‑CCAGCGCGTACTGGTGGCCGGAACGCTCAATAACGCCAAAA‑CGATCCGCATTGAACCGCCACTGACACTGACCa > 2:320063/1‑90 (MQ=255) gagaTGTCCCCCCGGCGCGTCCGGGGGGCCGGAACCCTAAAAACCGCAAAAA‑CGCCCCGCATTGAACCGCCACTGACACTGACCATTGaa < 1:52341/90‑1 (MQ=255) agttGTCCCGCCAGCGCGTCCGGGGGGCCGAACCGCAAAAAAACCCAAAAA‑CGACCCCCATTAACCCGCCACTGACACTGACCATTGAAc < 1:598929/87‑1 (MQ=255) agaTTTCCCGCCGGCGCGTAGGGGTGGCGGGAAC‑CCAAAAAACCCCAAAACCCCCCCGCATGGAACCGCCACTGACACTGACCATTGAAc < 1:900468/90‑1 (MQ=255) gttGTCCCGCCGGCGCGTACTGGTGGCCGGAACGCTCAATAACGCAAAAA‑CGACCCGCATTGAACCGCCACTGACACTGACCATTGAACa < 2:331789/88‑1 (MQ=255) ccGCCAGCGCGTAGTGGTGCCCGGAACCCAAAAAACCCCAAAAA‑CGACCCGCATTAAACCGCCACTGACACTGACCATTGAACAGTGTGa < 2:609525/90‑1 (MQ=255) ggcgcGTCCGGGGGGCCGAACCCCTAAAAACCGCAAAAA‑CGATCCGAATTAAACCGCCACTGACACTGACCATTGAACAGTGTGAACTgg < 1:320063/89‑1 (MQ=255) gccccgTCCTGGGGGCCGGAACCCTCAAAACCCCAAAAA‑CGCCCCGATTTGACCCGCCACTGACACTGACCATTGAACAGTGTGAACTgg < 2:533542/86‑1 (MQ=255) aGCGCGTACTGGTGGCCGGAACGCTCAATAACGCCAAAA‑CGATCCGCATTGAACCGCCACTGACACTGACCATTGAACAGTGTGAACTgg > 1:464623/1‑90 (MQ=255) aGCGCGTACGGGGGGCCGGACCGCCAAAAAACCCCAAAA‑CGACCCGCTTTGAACCGCCACTGACACTGACCATTGAACAGTGTGAACTgg < 2:135615/90‑1 (MQ=255) cgcgTACGGGGGGCGGGACCCCAAAAAAACCCAAAAC‑CGACCCGCATTGAACCGCCACTGACACTGACCATTGAACAGTGTGAACTGGTg < 1:130557/90‑1 (MQ=255) ggCCGGACCCCTAAAAACCGCAAAAA‑CGATCCGCTTTGAACCGCCACTGACACTGACCATTGAACAGTGTGAACTGGTGATCAAAgcggc < 1:40000/90‑1 (MQ=255) caaaac‑CGACCCGCATTAAACCGCCACTGACACTGACCATTGAACAGTGTGAACTGGTGATCAAAGCGGCGCGTAAGGCGCTGGCGGCCa < 2:421617/88‑1 (MQ=255) aaaac‑CGACCCGAATTGAACCGCCACTGACACTGACCATTGAACAGTGTGAACTGGTGATCAAAGCGGCGCGTAAGGCGCTGGCGGCCAt < 2:80140/89‑1 (MQ=255) | ATAACGAAATCGGCTATAACTTTGCCAGCGAGATGTTCCGCCAGCGCGTACTGGTGGCCGGAACGCTCAATAACGCCAAAA‑CGATCCGCATTGAACCGCCACTGACACTGACCATTGAACAGTGTGAACTGGTGATCAAAGCGGCGCGTAAGGCGCTGGCGGCCAT > NZ_CP009273/3214042‑3214207 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |