| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 17,050 | 0 | A | C | 54.5% | 11.1 / 13.7 | 11 | intergenic (‑147/‑439) | mokC/nhaA | type I toxin‑antitoxin system toxin MokC/Na+/H+ antiporter NhaA |
| Reads supporting (aligned to +/- strand): ref base A (5/0); new base C (0/6); total (5/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.16e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.28e-03 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GGCCTCGGGTTGATGGTAAAATATCACTCGGGGCTTTTCTCTATCTGCCGTTCAGCTAATGCCTGAGACAGACAGCCTCAAGCACCCGCCGCTATTATATCGCTCTCTTTAACCCATTTTGTTTTATCGATTCTAATCCTGAAGACGCCTCGCAT > NZ_CP009273/16967‑17121 | ggCCTCGGGTTGATGGTAAAATATCACTCGGGGCTTTTCTCTATCTGCCGTTCAGCTAATGCCTGAGACAGACAGCCTCAAGCACtcgcc > 2:543523/1‑90 (MQ=255)ggCCTCGGGTTGATGGTAAAATATCACTCGGGGCTTTTCTCTATCTGCCGTTCAGCTAATGCCTGAGACAGACAGCCTCAAGCACccgcc > 2:457281/1‑90 (MQ=255) cGGGTTGATGGTAAAATATCACTCGGGGCTTTTCTCTATCTGCCGTTCAGCTAATGCCTGAGACAGACAGCCTCAAGCACCCGCCGCtat > 1:732778/1‑90 (MQ=255) tGATGGTAAAATATCACTCGGGGCTTTTCTCTATCTGCCGTTCAGCTAATGCCTGAGACAGACAGCCTCAAGCACCCGCCGCTATTatat > 2:381607/1‑90 (MQ=255) gaaaaaTATCCCTGGGGTTTTTTCTCTATCTGCCGTTCAGCTAATGCCTGAGACAGACAGCCTCAAGCCCCCGCCGCTATTATATCGctc < 2:503590/88‑1 (MQ=255) gaaaaaTATCCCGGGGGGTTTTTCTCTTTCTCCCGTCCAGCTAATGCCGGAGACAGACACCCCCAAGCCCCCGCCGCTATTATATCGctc < 2:864643/88‑1 (MQ=255) aaaaaaTATCCCTGGGGGTTTTTCTCTTTCTGCCTTTCAGCTAATGCCTGAGACAGACACCCTCAAGCCCCCGCCGCTATTATATCGctc < 2:732778/88‑1 (MQ=255) aCTCGGGGCTTTTCTCTATCTGCCGTTCAGCTAATGCCTGAGACAGACAGCCTCAAGCACCCGCCGCTATTATATCGCTCTCTTTAAccc > 2:863988/1‑90 (MQ=255) tctATCTGCCGTTCAGCTAATGCCTGAGACAGACACCCTCAACCCCCCGCCGCTATTATATCGCTCTCTTTAACCCATTTTGTTTTATCg < 1:63051/90‑1 (MQ=255) gCCTGAGACAGACACCCAAAACCCCCCGCCGCTATTATATCGCTCTCTTTAACCCATTTTGTTTTATCGATTCTAATCCTGAAGACGCCt < 2:816893/90‑1 (MQ=255) agacagacACCCACAACCCCCCGCCGCTATTATATCGCTCTCTTTAACCCATTTTGTTTTATCGATTCTAATCCTGAAGACGCCTCGCAt < 1:381607/90‑1 (MQ=255) | GGCCTCGGGTTGATGGTAAAATATCACTCGGGGCTTTTCTCTATCTGCCGTTCAGCTAATGCCTGAGACAGACAGCCTCAAGCACCCGCCGCTATTATATCGCTCTCTTTAACCCATTTTGTTTTATCGATTCTAATCCTGAAGACGCCTCGCAT > NZ_CP009273/16967‑17121 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |