| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 4,172,812 | T→G | V547G (GTT→GGT) | rpoB → | DNA‑directed RNA polymerase subunit beta |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 4,172,812 | 0 | T | G | 94.1% | 179.0 / ‑1.0 | 51 | V547G (GTT→GGT) | rpoB | DNA‑directed RNA polymerase subunit beta |
| Reads supporting (aligned to +/- strand): ref base T (2/1); new base G (25/23); total (27/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01 | |||||||||||
CAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTC > NZ_CP009273/4172726‑4172893 | caacCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGGTCg < 1:476748/90‑1 (MQ=255) acCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCgag > 2:823949/1‑90 (MQ=255) acCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGGTCgag > 1:107920/1‑90 (MQ=255) cTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGGTCGAGACGTa > 1:199542/1‑90 (MQ=255) cTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGGTCGAGACGTa > 2:510070/1‑90 (MQ=255) cTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGGTCGAGACGTa > 1:834240/1‑90 (MQ=255) cTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGGTCGAGACGTa > 1:345189/1‑90 (MQ=255) tGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGGTCGAGACGTACAccc < 2:160140/90‑1 (MQ=255) gaTTCCGCCCAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGGTCGAGACGTACACCCGAc < 2:717689/90‑1 (MQ=255) gaTTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGGTCGAGACGTACACCCGAc < 1:178164/90‑1 (MQ=255) ccGCACAAACGTCGTATCTGGGTACTCGGTCCGGGCGGCTTACCCCGGGAAAAGGCAGCCTGCGAAGTTTGAGACGTACTCCCAACTCag > 1:752916/2‑89 (MQ=255) aCGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGGTCGAGACGTACACCCGAc > 2:773849/1‑86 (MQ=255) aCGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGGTCGAGACGTACACCCGAc < 1:773849/86‑1 (MQ=255) aCGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGGTCGAGACGTACACCCGACTCAc > 2:930453/1‑90 (MQ=255) aCGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGGTCGAGACGTACACCCGACTCAc > 1:867599/1‑90 (MQ=255) aCGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGGTCGAGACGTACACCCGACTCAc > 1:647949/1‑90 (MQ=255) aCGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGGTCGAGACGTACACCCGACTCAc > 1:638835/1‑90 (MQ=255) aCGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGGTCGAGACGTACACCCGACTCAc > 1:283170/1‑90 (MQ=255) caAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGGTCGAGACGTACACCCGACTCACTACgg < 2:811362/90‑1 (MQ=255) caAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGGTCGAGACGTACACCCGACTCACTACgg > 2:558580/1‑90 (MQ=255) cgtATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGGTCGAGACGTACACCCGACTCACTACGGTCGCGTa < 2:499295/90‑1 (MQ=255) aTCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGGTCGAGACGTACACCCGACTCACTACGGTCGCGTATGt > 2:348002/1‑90 (MQ=255) ctcCGCACTCGGCCCAGGCGCCCCGCCCCGTGAACGTGCAGGCTTCGAAGGTCGAGCCGTACCCCCGACTCCCTACGGTCGCGTATGTcc < 1:698179/90‑1 (MQ=255) ccGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGGTCgaga > 1:171382/1‑55 (MQ=255) ccGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGGTCgaga < 2:171382/55‑1 (MQ=255) gCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGGTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATc > 1:792869/1‑90 (MQ=255) gCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGGTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATc > 2:198169/1‑90 (MQ=255) cTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGGTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGaa < 2:638835/90‑1 (MQ=255) tCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGGTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGaaa < 2:511170/90‑1 (MQ=255) gCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGGTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAg < 1:29572/90‑1 (MQ=255) ggTCTGACCCGTGAACGTGCAGGCTTCGAAGGTCGAGACGTACACCCGAc > 1:100261/1‑50 (MQ=255) ggTCTGACCCGTGAACGTGCAGGCTTCGAAGGTCGAGACGTACACCCGAc < 2:100261/50‑1 (MQ=255) ggTCTGACCCGTGAACGTGCAGGCTTCGAAGGTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGt > 1:306723/1‑90 (MQ=255) gTCTGACCCGTGAACGTGCAGGCTTCGAAGGTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTc < 1:122286/90‑1 (MQ=255) tCTGACCCGTGAACGTGCAGGCTTCGAAGGTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTcc > 2:306842/1‑90 (MQ=255) tCTGACCCGTGAACGTGCAGGCTTCGAAGGTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTcc > 2:478623/1‑90 (MQ=255) tCTGACCCGTGAACGTGCAGGCTTCGAAGGTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTcc > 1:876723/1‑90 (MQ=255) cTGACCCGTGAACGTGCAGGCTTCGAAGGTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCg < 2:560393/90‑1 (MQ=255) gACCCGTGAACGTGCAGGCTTCGAAGGTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGaa > 1:258175/1‑90 (MQ=255) ccGTGAACGTGCAGGCTTCGAAGGTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACAt < 1:198169/90‑1 (MQ=255) gTGAACGTGCAGGCTTCGAAGGTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCg > 2:759551/1‑90 (MQ=255) aaCGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTc < 1:823949/90‑1 (MQ=255) aaCGTGCAGGCTTCGAAGGTCGAGACGTACCCCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTc < 1:560821/90‑1 (MQ=255) gCAGGCTTCGAAGGTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGaa > 2:300241/1‑60 (MQ=255) gCAGGCTTCGAAGGTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGaa < 1:300241/60‑1 (MQ=255) cAGGCTTCGAAGGTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCa > 1:700496/1‑90 (MQ=255) cAGGCTTCGAAGGTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCa < 1:813923/90‑1 (MQ=255) cAGGCTTCGAAGGTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCa < 2:258175/90‑1 (MQ=255) ggCTTCGAAGGTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAAc < 2:700496/90‑1 (MQ=255) cTTCGAAGGTCGAGACGTACCCCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAActc < 2:541752/90‑1 (MQ=255) cTTCGAAGGTCGAGACGTACCCCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAActc < 1:938126/90‑1 (MQ=255) | CAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTC > NZ_CP009273/4172726‑4172893 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |