| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,273,830 | 0 | A | C | 54.2% | 21.2 / 29.6 | 24 | T85T (ACA→ACC) | agaV | PTS N‑acetylgalactosamine transporter subunit IIB |
| Reads supporting (aligned to +/- strand): ref base A (8/3); new base C (0/13); total (8/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.24e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.43e-04 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
ATCGCCGTACGTTTCTGGACGCTGCAAAAAGTTATCGACAACATTCATCGCGCCGCCGATCGACAGAAAATCCTGCTGGTTTGTAAAACACCCGCCGATTTCCTGACGCTGGTGAAAGGTGGCGTTCCGGTGAATCGCATTAACGTTGGCAATATGCACTAC > NZ_CP009273/3273741‑3273902 | aTCGCCGTACGTTTCTGGACGCTGCAAAAAGTTATCGACAACATTCATCGCGCCGCCGATCGACAGAAAATCCTGCTGGTTTGTAAAaca > 2:32796/1‑90 (MQ=255) agTTTCTGGCCGCTGCAAAAAGTTATCGAAAACATTCATCGCGCCGCCGATCGAAAAAAAATCCTGCTGGTTTGTAAACCCCCCGCCGAt < 1:992862/89‑1 (MQ=255) cGCTGCAAAAAGTTATCGACAACATTCATCGCGCCGCCGATCGACAGAAAATCCTGCTGGTTTGTAAAACCCCCGCCGATTTCCTGACGc < 1:365925/90‑1 (MQ=255) cGCTGAAAAAATTTATCAACAACATTCACCGCCCCGCCGATCGACAAAAAATCCTGCTGGTTTGAAAAACCCCCGCCGATTTCCTGACGc < 1:798957/90‑1 (MQ=255) gCTGAAAAAATTTACCGAAAACATTCACCGCCCCGCCGATCGACAAAAAATCCTGCTGTTTTGAAAACCCCCCGCCGATTTCCTGAGGCt < 1:622901/90‑1 (MQ=255) cTGCAAAAAGTTATCGACAACATTCATCGCGCCGCCGATCGACAGAAAATCCTGCTGGTTTGTAAAACACCCGCCGATTTCCTGACGcct > 2:992862/1‑88 (MQ=255) cTGCAAAAAGTTATCGACAACATTCATCGCGCCGCCGATCGACAGAAAATCCTGCTGGTTTGTAAAACACCCGCCGATTTCCTGACGCTg > 2:794737/1‑90 (MQ=255) cTGCAAAAAGTTATCGACAACATTCATCGCGCCGCCGATCGACAGAAAATCCTGCTGGTTTGTAAAACACCCGCCGATTTCCTGACGCTg > 2:340380/1‑90 (MQ=255) taaaaaaaTTTTCCGAAAACATCCACCGCCCCGCCGATCGAAAAAAAATCCTGCTGGTTTGAAAAACCCCCGCCGATTTCCTGACGCTgg < 1:596249/87‑1 (MQ=255) aaaaaaaaTTTATCGACAACATCCCCCCCCCCGCCGACCGAAAAAAAATCCTGGGGTTTTGAAAACCCCCCGCCGATTTCCTGACGCTgg < 2:646951/87‑1 (MQ=255) gCAAAAAGTTATCGACAACATTCATCGCGCCGCCGATCGACAGAAAATCCTGCTGGTTTGTAAAACACCCGCCGATTTCCTGACGCTGGt > 2:617331/1‑90 (MQ=255) ttATCGACAACATTCATCGCGCCGCCGATCGACAGAAAATCCTGCTGGTTTGTAAAACACCCGCCGATTTCCTGACGCTGGTGAAAGGTg > 1:182501/1‑90 (MQ=255) tATCGACAACATTCATCGCGCCGCCGATCGACAGAAAATCCTGCTGGTTTGTAAAACACCCGCCGATTTCCTGACGCTGGTGAAAGGTgg < 1:32796/90‑1 (MQ=255) aTCGACAACATTCATCGCGCCGCCGATCGACAGAAAATCCTGCTGGTTTGTAAAACACCCGCCGATTTCCTGACGCTGGTGAAAGGTGGc > 1:145633/1‑90 (MQ=255) aTCGACAACATTCATCGCGCCGCCGATCGACAGAAAATCCTGCTGGTTTGTAAAACACCCGCCGATTTCCTGACGCTGGTGAAAGGTGGc > 2:277102/1‑90 (MQ=255) cATCGCGCCGCCGATCGACAGAAAATCCTGCTGGTTTGTAAAACCCCCGCCGATTTCCTGACGCTGGTGAAAGGTGGCGTTCCGGTGAAt < 1:31089/90‑1 (MQ=255) cATCGCGCCCCCGATCGACAGAAAATCCTGCTGGTTTGTAAAACACCCGCCGATTTCCTGACGCTGGTGAAAGGTGGCGTTCCGGTGAAt < 1:45416/90‑1 (MQ=255) accgcgCCGCCGATCGACAAAAAATCCTGCTGGTTTGTAAAACCCCCGCCGATTTCCTGACGCTGGTGAAAGGTGGCGTTCCGGTGAATc < 1:825631/88‑1 (MQ=255) ccccgccgATCGACAAAAAACCCTGTTGGTTTGTAAACCCCCCGCCGATTTCCTGACGCTGGTGAAAGGTGGCGTTCCGGTGAATCGCAt < 1:962199/88‑1 (MQ=255) gATCGAAAAAAAATCCTGCTGTTTTGAAAACCCCCCGCCGATTTCCTGACGCTGGTGAAAGGTGGCGTTCCGGTGAATCGCATTAACGtt < 1:783975/90‑1 (MQ=255) tCGACAGAAAATCCTGCTGGTTTGAAAACCACCCGCCGATTTCCTGACGCTGGTGAAAGGTGGCGTTCCGGTGAATCGCATTAACGTTgg < 1:120043/90‑1 (MQ=255) aaaaaaTCCTGCGGTTTTGAAAACCCCCCGCCGATTTCCTGACGCTGGTGAAAGGTGGCGTTCCGGTGAATCGCATTAACGTTGGCAata < 2:588000/88‑1 (MQ=255) aaaaaTCCTGCTGGTTTGTAAAACCCCCGCCGATTTCCTGACGCTGGTGAAAGGTGGCGTTCCGGTGAATCGCATTAACGTTGGCAatat < 1:170282/89‑1 (MQ=255) ccTGCTGTTTTGAAAACCCCCCGCCGATTTCCTGACGCTGGTGAAAGGTGGCGTTCCGGTGAATCGCATTAACGTTGGCAATATGCACTa < 1:966273/90‑1 (MQ=255) ctgctgGTTTGTAAAACACCCGCCGATTTCCTGACGCTGGTGAAAGGTGGCGTTCCGGTGAATCGCATTAACGTTGGCAATATGCACTAc > 2:541100/1‑90 (MQ=255) | ATCGCCGTACGTTTCTGGACGCTGCAAAAAGTTATCGACAACATTCATCGCGCCGCCGATCGACAGAAAATCCTGCTGGTTTGTAAAACACCCGCCGATTTCCTGACGCTGGTGAAAGGTGGCGTTCCGGTGAATCGCATTAACGTTGGCAATATGCACTAC > NZ_CP009273/3273741‑3273902 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |