| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 16,734 | 0 | A | C | 54.5% | 19.7 / 28.8 | 22 | intergenic (+177/+17) | BW25113_RS00080/mokC | IS4‑like element IS421 family transposase/type I toxin‑antitoxin system toxin MokC |
| Reads supporting (aligned to +/- strand): ref base A (9/1); new base C (0/12); total (9/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.01e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.45e-04 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GAACGCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGATCACTCCCCGCCGTTGCTCTTACTCGGATTCGTAAGCCGTGAAAACAGCAACCTCCGTCTGGCCAGTTCGGATGTGAACCTCACAGAGGTCT > NZ_CP009273/16651‑16823 | gaaCGGGGGGCATCGTCTTAACGAGGCACGGGGGCGTCGCTTTCTTCAGATGTTTCACCCCTTAAGTTAGCGTTTATGGGATcccccccc < 2:983156/90‑1 (MQ=11) aCGCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGATCACTCCccgc > 1:867961/1‑90 (MQ=39) aCGCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGATCACTCCccgc > 1:627791/1‑90 (MQ=39) gAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGTTTATGGGATCACTCCCCGCCGtt < 1:439659/90‑1 (MQ=255) tCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGATCACTCCCCGCCGTTGCTCtt > 1:969161/1‑90 (MQ=255) cGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGTTTATGGGATCCCTCCCCGCCGTTGCTCTTa < 1:868205/90‑1 (MQ=255) ggCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGATCACTCCCCGCCGTTGCTCTTACTCGGATTCGt > 1:34852/1‑90 (MQ=255) gCACGGAGGCGTCGCATTCTTCAGTTGGTTCACCCTTTAAGTTAGCGTTTATGGGACCCCTCCCCGCCGTTGCTCTTACTCGGATTCGTa < 1:866360/90‑1 (MQ=21) gCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGATCACTCCCCGCCGTTGCTCTTACTCGGATTCGTa > 1:633318/1‑90 (MQ=255) tCGCATTCTTCAGGGGGTTCCACCCTTAAGTTGGCCTTTTGGGGACCCCCCCCCGCCGTTGCTCTTACTCGGATTCGTAAGCCGTGaaaa < 2:136210/90‑1 (MQ=255) ggTTCAACCCTTAAGTTAGCGCTTATGGGATCACTCCCCGCCGTTGCTCTTACTCGGATTCGTAAGCCGTGAAAACAGCAACCTCCGTCt > 2:534928/1‑90 (MQ=255) caccccTTAAGTTGGCGTTTAGGGGATCCCCCCCCGCCGTTGCTCTTACTCGGATTCGTAAGCCGTGAAAACAGCAACCTCCGTCTGGcc < 2:222304/87‑1 (MQ=38) caccccTTAAGTTAGCGTTTAGGGGATCCCCCCCCGCCGTTGCTCTTACTCGGATTCGTAAGCCGTGAAAACAGCAACCTCCGTCTGGcc < 2:572389/87‑1 (MQ=38) caccccTTAAGTTAGCGTTTAGGGGACCCCCCCCCGCCGTTGCTCTTACTCGGATTCGTAAGCCGTGAAAACAGCAACCTCCGTCTGGcc < 2:627791/87‑1 (MQ=38) caccccTTAAGTTAGCGCTTAGGGGATCCCCCCCCGCCGTTGCTCTTACTCGGATTCGTAAGCCGTGAAAACAGCAACCTCCGTCTGGcc < 1:145855/87‑1 (MQ=39) caccccTAAATTTAGCGCTTAGGGGACCCCCCCCCGCCGTTGCTCTTACTCGGATTCGTAAGCCGTGAAAACAGCAACCTCCGTCTGGcc < 2:34852/87‑1 (MQ=37) aaaCCCTTAAGTAAGCCCTTGTGAAAACCCCCCCCGCCGTTGCTCTTCCTCGGATTCGTAAGCCGTGAAAACAGCAACCTCCGTCTGGcc < 2:637645/89‑1 (MQ=25) cTTATGTTGGCGCTTGTGGACCCCCCCCCCGCCGTTGCTCTTACTCGGATTCGTAAGCCGTGAAAACAGCAACCTCCGTCTGGCCAGTTc < 2:798601/90‑1 (MQ=25) tAGCGCTTATGGGATCACTCCCCGCCGTTGCTCTTACTCGGATTCGTAAGCCGTGAAAACAGCAACCTCCGTCTGGCCAGTTCGGAtgtg > 2:922514/1‑90 (MQ=255) ggATCCCCCCCCGCCGTTGCTCTTACTCGGATTCGTAAGCCGTGAAAACAGCACCCTCCGTCTGGCCAGTTCGGATGTGAACCTCACaga < 1:909880/90‑1 (MQ=255) ggATCCCCCCCCGCCGTTGCTCTTACTCGGATTCGTAAGCCGTGAAAACAGCAACCTCCGTCTGGCCAGTTCGGATGTGAACCTCACaga < 1:760780/90‑1 (MQ=255) gATCACTCCCCGCCGTTGCTCTTACTCGGATTCGTAAGCCGTGAAAACAGCAACCTCCGTCTGGCCAGTTCGGATGTGAACCTCACagag > 2:481974/1‑90 (MQ=255) cACTCCCCGCCGTTGCTCTTACTCGGATTCGTAAGCCGTGAAAACAGCAACCTCCGTCTGGCCAGTTCGGATGTGAACCTCACAGAGGTc > 1:139812/1‑90 (MQ=255) aCTCCCCGCCGTTGCTCTTACTCGGATTCGTAAGCCGTGAAAACAGCAACCTCCGTCTGGCCAGTTCGGATGTGAACCTCACAGAGGTCt > 1:1011577/1‑90 (MQ=255) | GAACGCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGATCACTCCCCGCCGTTGCTCTTACTCGGATTCGTAAGCCGTGAAAACAGCAACCTCCGTCTGGCCAGTTCGGATGTGAACCTCACAGAGGTCT > NZ_CP009273/16651‑16823 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |