Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	49,884	C→T	P21L (CCG→CTG)	folA →	type 3 dihydrofolate reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	49,884	0	C	T	100.0%	91.9 / NA	31	P21L (CCG→CTG)	folA	type 3 dihydrofolate reductase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (9/22);  total (9/22)

TTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAA  >  NZ_CP009273/49801‑49970                                                                                    |                                                                                       ttttttttATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCTGTGGaa                                                                                  <  2:668017/90‑1 (MQ=255)                     cAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCTGTGGAACCTGCCTGCCGATCTCGccc                                                              <  2:504684/90‑2 (MQ=255)                     cAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCTGTGGAACCTGCCTGCCGATCTCGccc                                                              <  1:474458/90‑2 (MQ=255)                     cAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCTGTGGAACCTGCCTGCCGATCTCGccc                                                              <  1:78147/90‑2 (MQ=255)                     cAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCTGTGGAACCTGCCTGCCGATCTCGccc                                                              <  1:957586/90‑2 (MQ=255)                            cAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCTGTGGAACCTGCCTGCCGATCTCGCCCGGTTTaa                                                       >  2:362891/1‑90 (MQ=255)                             aGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCTGTGGAACCTGCCTGCCGATCTCGCCCGGTTTaaa                                                      >  1:345710/1‑90 (MQ=255)                                  gATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCTGTGGAACCTGCCTGCCGATCTCGCCCGGTTTAAACGCaa                                                 <  1:281035/90‑1 (MQ=255)                                   aTTGCGGCGTTAGCGGTAGATCGCGTTATCGGCAGGGAAAACGCCAGGCTGTGGAACCTGCCTGCCGATCTCGCCCGGTTTAAACGCAac                                                <  2:1039963/90‑1 (MQ=255)                                      gcggcgTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCTGTGGAACCTGCCTGCCGATCTCGCCCGGTTTAAACGCAACAcc                                             >  2:680382/1‑90 (MQ=255)                                       cggcgTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCTGTGGAACCTGCCTGCCGATCTCGCCCGGTTTAAACGCAACACCt                                            <  2:970307/90‑1 (MQ=255)                                       cggcgTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCTGTGGAACCTGCCTGCCGATCTCGCCCGGTTTAAACGCAACACCt                                            <  1:305612/90‑1 (MQ=255)                                        ggcgTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCTGTGGAACCTGCCTGCCGATCTCGCCCGGTTTAAACGCAac                                                >  2:856004/1‑85 (MQ=255)                                        ggcgTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCTGTGGAACCTGCCTGCCGATCTCGCCCGGTTTAAACGCAac                                                <  1:856004/85‑1 (MQ=255)                                        ggcgTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCTGTGGAACCTGCCTGCCGATCTCGCCCGGTTTAAACGCAACACCtt                                           >  1:648114/1‑90 (MQ=255)                                              aGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCTGTGGAACCTGCCTGCCGATCTCGCCCGGTTTAAACGCAACACCTtaaataa                                     <  2:234787/90‑1 (MQ=255)                                                       tCGCGTTATCGGCATGGAAAACGCCATGCTGTGGAACCTGCCTGCCGATCTCGCCCGGTTTAAACGCAACACCTTAAATAAACCCGTGAt                            >  2:951791/1‑90 (MQ=255)                                                         gcgTTATCGGCATGGAAAACGCCATGCTGTGGAACCTGCCTGCCGATCTCGCCCGGTTTAAACGCAACACCTTAAATAAACCCGTGATTa                          >  2:826419/1‑90 (MQ=255)                                                          cgTTATCGGCATGGAAAACGCCATGCTGTGGAACCTGCCTGCCGATCTCGCCCGGTTTAAACGCAACACCTTAAATAAACCCGTGATTAt                         <  1:826419/90‑1 (MQ=255)                                                            ttATCGGCATGGAAAACGCCATGCTGTGGAACCTGCCTGCCGATCTCGCCCGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATgg                       <  1:456488/90‑1 (MQ=255)                                                             tATGGGCATGAAAACCCCCATGCTGGGAACCCCCCCTGCCGATCTCGCCCGGTTTAAACGCAACACCTTAAATAACCCCGTGATTATggg                      <  2:788226/90‑1 (MQ=255)                                                                    agggAAACCCCCATGCTGTGGACCCTGCCTGCCGATCTCGCCCGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCAt               <  2:195808/88‑1 (MQ=255)                                                                    aTGGAAAACGCCATGCTGTGGAACCTGCCTGCCGATCTCGCCCGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCAt               <  1:922037/90‑1 (MQ=255)                                                                    aTGGAAAACCCCATGCTGTGGACCCTGCCTGCCGATCTCGCCCGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCAt               <  2:385452/90‑1 (MQ=255)                                                                     tgaaaaaAGCTATGCTGTGAAACCGCCCTGCCGATCTCGCCCGGTTTAAACGCAACCCCTTAAATAAACCCGTGATTATGGGCCGCCATa              <  2:1029017/87‑1 (MQ=255)                                                                        aaaaCGCCATGCTGTGGAACCTGCCTGCCGATCTCGCCCGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCt           <  1:282770/90‑1 (MQ=255)                                                                            cGCCATGCTGTGGAACCTGCCTGCCGATCTCGCCCGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGa       >  2:669623/1‑90 (MQ=255)                                                                               cATGCTGTGGAACCTGCCTGCCGATCTCGCCCGGTTTAAACGCAACACCTtaaataa                                     <  1:1037260/57‑1 (MQ=255)                                                                               cATGCTGTGGAACCTGCCTGCCGATCTCGCCCGGTTTAAACGCAACACCTtaaataa                                     >  2:1037260/1‑57 (MQ=255)                                                                                 tGCTGTGGACCCTCCCTGCCGATCTCGCCCGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGaatcaa  <  2:792275/90‑1 (MQ=255)                                                                                 tGCTGTGGAACCTGCCTGCCGATCTCGCCCGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGaatcaa  <  1:362891/90‑1 (MQ=255)                                                                                    |                                                                                       TTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAA  >  NZ_CP009273/49801‑49970

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑