Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	49,910	T→C	W30R (TGG→CGG)	folA →	type 3 dihydrofolate reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	49,910	0	T	C	100.0%	138.0 / NA	38	W30R (TGG→CGG)	folA	type 3 dihydrofolate reductase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (14/24);  total (14/24)

CAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCAGGACGCAAA  >  NZ_CP009273/49828‑49996                                                                                   |                                                                                       cAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCTGTGGAACCTGCCTGCCGATCTCGCCCGGTTTaa                                                                                 >  2:362891/1‑90 (MQ=255)  aGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCTGTGGAACCTGCCTGCCGATCTCGCCCGGTTTaaa                                                                                >  1:345710/1‑90 (MQ=255)       gATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCTGTGGAACCTGCCTGCCGATCTCGCCCGGTTTAAACGCaa                                                                           <  1:281035/90‑1 (MQ=255)        aTTGCGGCGTTAGCGGTAGATCGCGTTATCGGCAGGGAAAACGCCAGGCTGTGGAACCTGCCTGCCGATCTCGCCCGGTTTAAACGCAac                                                                          <  2:1039963/90‑1 (MQ=255)           gcggcgTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCTGTGGAACCTGCCTGCCGATCTCGCCCGGTTTAAACGCAACAcc                                                                       >  2:680382/1‑90 (MQ=255)            cggcgTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCTGTGGAACCTGCCTGCCGATCTCGCCCGGTTTAAACGCAACACCt                                                                      <  2:970307/90‑1 (MQ=255)            cggcgTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCTGTGGAACCTGCCTGCCGATCTCGCCCGGTTTAAACGCAACACCt                                                                      <  1:305612/90‑1 (MQ=255)             ggcgTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCTGTGGAACCTGCCTGCCGATCTCGCCCGGTTTAAACGCAac                                                                          >  2:856004/1‑85 (MQ=255)             ggcgTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCTGTGGAACCTGCCTGCCGATCTCGCCCGGTTTAAACGCAac                                                                          <  1:856004/85‑1 (MQ=255)             ggcgTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCTGTGGAACCTGCCTGCCGATCTCGCCCGGTTTAAACGCAACACCtt                                                                     >  1:648114/1‑90 (MQ=255)                   aGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCTGTGGAACCTGCCTGCCGATCTCGCCCGGTTTAAACGCAACACCTtaaataa                                                               <  2:234787/90‑1 (MQ=255)                            tCGCGTTATCGGCATGGAAAACGCCATGCTGTGGAACCTGCCTGCCGATCTCGCCCGGTTTAAACGCAACACCTTAAATAAACCCGTGAt                                                      >  2:951791/1‑90 (MQ=255)                              gcgTTATCGGCATGGAAAACGCCATGCTGTGGAACCTGCCTGCCGATCTCGCCCGGTTTAAACGCAACACCTTAAATAAACCCGTGATTa                                                    >  2:826419/1‑90 (MQ=255)                               cgTTATCGGCATGGAAAACGCCATGCTGTGGAACCTGCCTGCCGATCTCGCCCGGTTTAAACGCAACACCTTAAATAAACCCGTGATTAt                                                   <  1:826419/90‑1 (MQ=255)                                 ttATCGGCATGGAAAACGCCATGCTGTGGAACCTGCCTGCCGATCTCGCCCGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATgg                                                 <  1:456488/90‑1 (MQ=255)                                  tATGGGCATGAAAACCCCCATGCTGGGAACCCCCCCTGCCGATCTCGCCCGGTTTAAACGCAACACCTTAAATAACCCCGTGATTATggg                                                <  2:788226/90‑1 (MQ=255)                                         agggAAACCCCCATGCTGTGGACCCTGCCTGCCGATCTCGCCCGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCAt                                         <  2:195808/88‑1 (MQ=255)                                         aTGGAAAACGCCATGCTGTGGAACCTGCCTGCCGATCTCGCCCGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCAt                                         <  1:922037/90‑1 (MQ=255)                                         aTGGAAAACCCCATGCTGTGGACCCTGCCTGCCGATCTCGCCCGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCAt                                         <  2:385452/90‑1 (MQ=255)                                          tgaaaaaAGCTATGCTGTGAAACCGCCCTGCCGATCTCGCCCGGTTTAAACGCAACCCCTTAAATAAACCCGTGATTATGGGCCGCCATa                                        <  2:1029017/87‑1 (MQ=255)                                             aaaaCGCCATGCTGTGGAACCTGCCTGCCGATCTCGCCCGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCt                                     <  1:282770/90‑1 (MQ=255)                                                 cGCCATGCTGTGGAACCTGCCTGCCGATCTCGCCCGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGa                                 >  2:669623/1‑90 (MQ=255)                                                    cATGCTGTGGAACCTGCCTGCCGATCTCGCCCGGTTTAAACGCAACACCTtaaataa                                                               <  1:1037260/57‑1 (MQ=255)                                                    cATGCTGTGGAACCTGCCTGCCGATCTCGCCCGGTTTAAACGCAACACCTtaaataa                                                               >  2:1037260/1‑57 (MQ=255)                                                      tGCTGTGGACCCTCCCTGCCGATCTCGCCCGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGaatcaa                            <  2:792275/90‑1 (MQ=255)                                                      tGCTGTGGAACCTGCCTGCCGATCTCGCCCGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGaatcaa                            <  1:362891/90‑1 (MQ=255)                                                        ctgTGGACCCTGCCTGCCGATCTCGCCCGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGaatcaatc                          <  1:960783/88‑1 (MQ=255)                                                        ctgTGGAACCTGCCTGCCGATCTCGCCCGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGaatcaatc                          <  2:345710/88‑1 (MQ=255)                                                               aCCTGCCTGCCGATCTCGCCCGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGgtcgtc                   >  1:411673/1‑90 (MQ=255)                                                                 ctgcctgcCGATCTCGCCCGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCg                 <  2:757383/90‑1 (MQ=255)                                                                 ctgcctgcCGATCTCGCCCGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCg                 >  1:601487/1‑90 (MQ=255)                                                                  tgcctgccGATCTCGCCCGGTTTAAACGCAACGCCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGt                <  2:648114/90‑1 (MQ=255)                                                                  tgcctgccGATCTCGCCCGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGt                >  2:663513/1‑90 (MQ=255)                                                                    cctgccGATCTCGCCCGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATa                                        >  2:521363/1‑64 (MQ=255)                                                                    cctgccGATCTCGCCCGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATa                                        <  1:521363/64‑1 (MQ=255)                                                                            tctcGCCCGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCAGGACg      >  2:113651/1‑90 (MQ=255)                                                                                gCCCGGTTTAAACGCAACCCCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCAGGACGCaaa  <  2:140912/90‑1 (MQ=255)                                                                                gCCCGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCAGGACGCaaa  <  1:65055/90‑1 (MQ=255)                                                                                   |                                                                                       CAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCAGGACGCAAA  >  NZ_CP009273/49828‑49996

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑