Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	3,754,231	G→T	R127S (CGC→AGC)	selA ←	L‑seryl‑tRNA(Sec) selenium transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	3,754,231	0	G	T	100.0%	243.6 / NA	61	R127S (CGC→AGC)	selA	L‑seryl‑tRNA(Sec) selenium transferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (29/32);  total (29/32)

CCGCCGCATTGTTATTGACGATACAGGCATCTTCCGCCCCCGTAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGTCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCTGCGCAACGGCTTCCACCGCGGCTTCCGCCTG  >  NZ_CP009273/3754144‑3754312                                                                                        |                                                                                  ccgccgcATTGTTATTGACGATACAGGCATCTTCCGCCCCCGTAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCTTc                                                                                 >  1:330786/1‑90 (MQ=255)  cgccgcATTGTTATTGACGATACAGGCATCTTCCGCCCCCGTAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCTTcc                                                                                <  2:267508/90‑1 (MQ=255)  cgccgcATTGTTATTGACGATACAGGCATCTTCCGCCCCCGTAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCTTcc                                                                                <  2:220289/90‑1 (MQ=255)   gccgcATTGTTATTGACGATACAGGCATCTTCCGCCCCCGTAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCTTCCg                                                                               >  1:775816/1‑90 (MQ=255)       cATTGTTATTGACGATACAGGCATCTTCCGCCCCCGTAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCTTCCGGcgt                                                                           <  2:976725/90‑1 (MQ=255)       cATTGTTATTGACGATACAGGCATCTTCCGCCCCCGTAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCTTCCGGcgt                                                                           <  2:302528/90‑1 (MQ=255)       cATTGTTATTGACGATACAGGCATCTTCCGCCCCCGTAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCTTCCGGcgt                                                                           <  2:188556/90‑1 (MQ=255)       cATTGTTATTGACGATACAGGCATCTTCCGCCCCCGTAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCTTCCGGcgt                                                                           <  1:977940/90‑1 (MQ=255)            ttATTGACGATACAGGCATCTTCCGCCCCCGTAATCCGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCTTCCGGCGTCGTcc                                                                      <  2:555416/90‑1 (MQ=255)            ttATTGACGATACAGGCATCTTCCGCCCCCGTAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCTTCCGGCGTCGTcc                                                                      <  1:1041161/90‑1 (MQ=255)            ttATTGACGATACAGGCATCTTCCGCCCCCGTAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCTTCCGGCGTCGTcc                                                                      <  2:89655/90‑1 (MQ=255)            ttATTGACGATACAGGCATCTTCCGCCCCCGTAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCTTCCGGCGTCGTcc                                                                      <  2:838805/90‑1 (MQ=255)            ttATTGACGATACAGGCATCTTCCGCCCCCGTAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCTTCCGGCGTCGTcc                                                                      <  1:55268/90‑1 (MQ=255)              aTTGACGATACAGGCATCTTCCGCCCCCGTAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCTTCCGGCGTCGTCCAg                                                                    <  1:1009560/90‑1 (MQ=255)              aTTGACGATACAGGCATCTTCCGCCCCCGTAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCTTCCGGCGTCGTCCAg                                                                    <  1:967458/90‑1 (MQ=255)                  aCGATACAGGCATCTTCCGCCCCCGTAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCTTCCGGCGTCGTCCAGATCa                                                                <  1:169336/90‑1 (MQ=255)                   cGATACAGGCATCTTCCGCCCCCGTAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCTTCCGGCGTCGTCCAGATCAt                                                               >  2:80544/1‑90 (MQ=255)                   cGATACAGGCATCTTCCGCCCCCGTAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCTTCCGGCGTCGTCCAGATCAt                                                               <  1:80544/90‑1 (MQ=255)                    gATACAGGCATCTTCCGCCCCCGTAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCTTCCGGCGTCGTCCAGATCATa                                                              <  2:668095/90‑1 (MQ=255)                     aTACAGGCATCTTCCGCCCCCGTAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCTTCCGGCGTCGTCCAGATCATAc                                                             >  1:562021/1‑90 (MQ=255)                     aTACAGGCATCTTCCGCCCCCGTAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCTTCCGGCGTCGTCCAGATCATAc                                                             >  1:197579/1‑90 (MQ=255)                     aTACAGGCATCTTCCGCCCCCGTAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCTTCCGGCGTCGTCCAGATCATAc                                                             >  2:1028760/1‑90 (MQ=255)                         aGGCATCTTCCGCCCCCGTAATACGGCACAGCCGCTGCGCCAGCGCCCGATCGCGATGTCCGCTTCCGGCGTCGTCCAGATCATACTCGa                                                         <  2:585733/90‑1 (MQ=255)                         aGGCATCTTCCGCCCCCGTAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCTTCCGGCGTCGTCCAGATCATACTCGa                                                         <  1:119012/90‑1 (MQ=255)                             aTCTTCCGCCCCCGTAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCTTCCGGCGTCGTCCAGATCATACTCGAGGGt                                                     >  2:146219/1‑90 (MQ=255)                               cTTCCGCCCCCGTAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCTTCCGGCGTCGTcc                                                                      >  2:581156/1‑71 (MQ=255)                               cTTCCGCCCCCGTAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCTTCCGGCGTCGTcc                                                                      <  1:581156/71‑1 (MQ=255)                               cTTCCGCCCCCGTAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCTTCCGGCGTCGTCCAGATCATACTCGAGGGTCa                                                   >  1:191723/1‑90 (MQ=255)                               cTTCCGCCCCCGTAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCTTCCGGCGTCGTCCAGATCATACTCGAGGGTCa                                                   >  1:71207/1‑90 (MQ=255)                               cTTCCGCCCCCGTAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCTTCCGGCGTCGTCCAGATCATACTCGAGGGTCa                                                   >  1:977348/1‑90 (MQ=255)                                ttCCGCCCCCGTAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCTTCCGGCGTCGTCCAGATCATACTCGAGGGTCAc                                                  <  2:500544/90‑1 (MQ=255)                                ttCCGCCCCCGTAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCTTCCGGCGTCGTCCAGATCATACTCGAGGGTCAc                                                  <  2:967790/90‑1 (MQ=255)                                      ccccGTAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCTTCCGGCGTCGTCCAGATCATACTCGAGGGTCACTGGCGa                                            <  1:797749/90‑1 (MQ=255)                                        ccGTAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCTTCCGGCGTCGTCCAGATCATACTCGAGGGTCACTGGCGAAc                                          >  2:723854/1‑90 (MQ=255)                                         cGTAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCTTCCGGCGTCGTCCAGATCATACTCGAGGGTCACTGGCGAACg                                         >  1:376677/1‑90 (MQ=255)                                            aaTACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCTTCCGGCGTCGTCCAGATCATACTCGAGGGTCACTGGCGAACGCAt                                      <  1:848797/90‑1 (MQ=255)                                                  gCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCTTCCGGCGTCGTCCAGATCATACTCGAGGGTCACTGGCGa                                            >  2:751540/1‑78 (MQ=255)                                                  gCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCTTCCGGCGTCGTCCAGATCATACTCGAGGGTCACTGGCGa                                            <  1:751540/78‑1 (MQ=255)                                                  gCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCTTCCGGCGTCGTCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCTg                                >  1:313817/1‑90 (MQ=255)                                                   cacaGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCTTCCGGCGTCGTCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCTgc                               >  1:399937/1‑90 (MQ=255)                                                   cacaGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCTTCCGGCGTCGTCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCTgc                               >  1:689865/1‑90 (MQ=255)                                                    acaGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCTTCCGGCGTCGTcc                                                                      >  1:6278/1‑50 (MQ=255)                                                    acaGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCTTCCGGCGTCGTcc                                                                      <  2:6278/50‑1 (MQ=255)                                                     cagcagCTGCGCCAGCGCCCGATCGCGATGTCCGCTTCCGGCGTCGCCCAGGTCATACTCGGGGGTCTCCGGCGAACGCATACCCTgcgc                             >  2:738276/1‑90 (MQ=255)                                                       gcagcTGCGCCAGCGCCCGATCGCGATGTCCGCTTCCGGCGTCGTCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCTGCGCaa                           >  2:296806/1‑90 (MQ=255)                                                       gcagcTGCGCCAGCGCCCGATCGCGATGTCCGCTTCCGGCGTCGTCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCTGCGCaa                           >  1:910606/1‑90 (MQ=255)                                                       gcagcTGCGCCAGCGCCCGATCGCGATGTCCGCTTCCGGCGTCGTCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCTGCGCaa                           <  2:562021/90‑1 (MQ=255)                                                          gcTGCGCCAGCGCCCGATCGCGATGTCCGCTTCCGGCGTCGTCCAGATCa                                                                <  2:1062674/50‑1 (MQ=255)                                                          gcTGCGCCAGCGCCCGATCGCGATGTCCGCTTCCGGCGTCGTCCAGATCa                                                                >  1:1062674/1‑50 (MQ=255)                                                          gcTGCGCCAGCGCCCGATCGCGATGTCCGCTTCCGGCGTCGTCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCTGCGCAACgg                        >  2:792135/1‑90 (MQ=255)                                                            tGCGCCAGCGCCCGATCGCGATGTCCGCTTCCGGCGTCGTCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCTGCGCAACGGCt                      >  1:910615/1‑90 (MQ=255)                                                               gcCAGCGCCCGATCGCGATGTCCGCTTCCGGCGTCGTCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCTGCGCAACGGCTTcc                   <  2:503750/90‑1 (MQ=255)                                                                 cAGCGCCCGATCGCGATGTCCGCTTCCGGCGTCGTCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCTGCGCAACGGCTTCCAc                 >  1:610644/1‑90 (MQ=255)                                                                       ccGATCGCGATGTCCGCTTCCGGCGTCGTCCAGATCATACTCGAGGGTCACTGGCGa                                            <  1:617001/57‑1 (MQ=255)                                                                       ccGATCGCGATGTCCGCTTCCGGCGTCGTCCAGATCATACTCGAGGGTCACTGGCGa                                            >  2:617001/1‑57 (MQ=255)                                                                         gATCGCGATGTCCGCTTCCGGCGTCGTCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCTGCGCAACGGCTTCCACCGCGGCtt         >  1:812322/1‑90 (MQ=255)                                                                         gATCGCGAGGTCCGCTTCCGGCGTCGTCCAGATCATCCTCGAGGGTCACTGGCGAACGCATAGCCTGCGCAACGGCTTCCCCCGCGGCtt         <  2:946623/90‑1 (MQ=255)                                                                           tCGCGATGTCCGCTTCCGGCGTCGTCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCTGCGCAACGGCTTCCACCGCGGCTTcc       <  1:171116/90‑1 (MQ=255)                                                                                aTGTCCGCTTCCGGCGTCGTCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCTGCGCAACGGCTTCCACCGCGGCTTCCGCCTg  >  1:576668/1‑90 (MQ=255)                                                                                    ccGCTTCCGGCGTCGTCCAGATCATACTCGAGGGTCa                                                   >  2:580043/1‑37 (MQ=38)                                                                                    ccGCTTCCGGCGTCGTCCAGATCATACTCGAGGGTCa                                                   <  1:580043/37‑1 (MQ=255)                                                                                        |                                                                                  CCGCCGCATTGTTATTGACGATACAGGCATCTTCCGCCCCCGTAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGTCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCTGCGCAACGGCTTCCACCGCGGCTTCCGCCTG  >  NZ_CP009273/3754144‑3754312

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑