| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 4,172,812 | T→G | V547G (GTT→GGT) | rpoB → | DNA‑directed RNA polymerase subunit beta |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 4,172,812 | 0 | T | G | 98.3% | 220.1 / ‑3.6 | 59 | V547G (GTT→GGT) | rpoB | DNA‑directed RNA polymerase subunit beta |
| Reads supporting (aligned to +/- strand): ref base T (1/0); new base G (25/33); total (26/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.41e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.19e-01 | |||||||||||
CAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCT > NZ_CP009273/4172726‑4172896 | caacCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGGTCg < 1:703297/90‑1 (MQ=255)caacCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGGTCg < 1:466957/90‑1 (MQ=255) aacCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGGTCga < 2:955104/90‑1 (MQ=255) aacCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGGTCga < 1:147015/90‑1 (MQ=255) aacCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGGTCga < 2:133140/90‑1 (MQ=255) aacCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGGTCga < 1:543619/90‑1 (MQ=255) acCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGGTCgag < 1:175254/90‑1 (MQ=255) acCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGGTCgag > 2:701380/1‑90 (MQ=255) ccGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGGTCGAGAc > 2:171066/1‑90 (MQ=255) cTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGGTCGAGACGTa > 1:118609/1‑90 (MQ=255) cTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGGTCGAGACGTa > 2:532564/1‑90 (MQ=255) cTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGGTCGAGACGTa > 2:274507/1‑90 (MQ=255) cTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGGTCGAGACGTa > 1:1016184/1‑90 (MQ=255) tCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGGTCGAGACGTacac < 2:607828/90‑1 (MQ=255) cTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGGTCGAGACGTACAcc > 1:947497/1‑90 (MQ=255) tGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGGTCGAGACGTACGccc < 2:118609/90‑1 (MQ=255) tGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGGTCGAGACGTACAccc < 2:130337/90‑1 (MQ=255) gaTTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGGTCGAGACGTACACCCGAc < 1:831192/90‑1 (MQ=255) gaTTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGGTCGAGACGTACACCCGAc < 1:746234/90‑1 (MQ=255) aCGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCAc > 2:749656/1‑90 (MQ=255) aCGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGGTCGAGACGTACACCCGACTCAc > 2:235933/1‑90 (MQ=255) aCGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGGTCGAGACGTACACCCGACTCAc > 2:227832/1‑90 (MQ=255) aCGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGGTCGAGACGTACACCCGACTCAc > 1:758224/1‑90 (MQ=255) aCGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGGTCGAGACGTACACCCGACTCAc > 1:426119/1‑90 (MQ=255) aaaCGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACATGCAGGCTTCGAAGGTCGAGACGTACACCCGACTCACTACGGt > 2:839631/1‑90 (MQ=255) aaCGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGGTCGAGACGTACACCCGACTCATTACGGTc < 2:779843/90‑1 (MQ=255) aaCGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGGTCGAGACGTACACCCGACTCACTACGGTc < 2:689202/90‑1 (MQ=255) gtATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGGTCGAGACGTACACCCGACTCACTACGGTCGCGTAt > 2:283903/1‑90 (MQ=255) aTCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGGTCGAGACGTACACCCGACTCACTACGGTCGCGTATGt > 1:49708/1‑90 (MQ=255) ctcCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGGTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTcc < 2:577793/90‑1 (MQ=255) ctcCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGGTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTcc < 2:197454/90‑1 (MQ=255) aCTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGGTCGAGACGTACACCCGAc < 1:779787/63‑1 (MQ=255) aCTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGGTCGAGACGTACACCCGAc > 2:779787/1‑63 (MQ=255) cTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGGTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGaa < 1:532564/90‑1 (MQ=255) cGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGGTCgaga < 2:336952/48‑1 (MQ=255) cGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGGTCgaga > 1:336952/1‑48 (MQ=255) cGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGGTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAAc > 2:380031/1‑90 (MQ=255) ggCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGGTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGaa > 2:706020/1‑90 (MQ=255) ggCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGGTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGaa < 2:639478/90‑1 (MQ=255) gCGGTCTGACCCGTGAACGTGCGGGCTTCGAAGGTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAg < 2:544756/90‑1 (MQ=255) tCTGACCCGTGAACGTGCAGGCTTCGAAGGTCGAGACGTACACCCGAc < 1:311034/48‑1 (MQ=255) tCTGACCCGTGAACGTGCAGGCTTCGAAGGTCGAGACGTACACCCGAc > 2:311034/1‑48 (MQ=255) tCTGACCCGTGAACGTGCAGGCTTCGAAGGTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTcc > 2:594813/1‑90 (MQ=255) tCTGACCCGTGAACGTGCAGGCTTCGAAGGTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTcc > 2:548529/1‑90 (MQ=255) cTGCCCCGTGAACGTGCAGGCTTCGAAGGTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCg < 1:673767/90‑1 (MQ=255) cTGACCCGTGAACGTGCAGGCTTCGAAGGTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCg < 1:322996/90‑1 (MQ=255) tGACCCGTGAACGTGCAGGCTTCGAAGGTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGa < 2:1016184/90‑1 (MQ=255) aaCGTGCAGGCTTCGAAGGTCGAGACGTTCACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTc < 2:62841/90‑1 (MQ=255) aaCGTGCAGGCTTCGAAGGTCGAGACGTACCCCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTc < 2:1016429/90‑1 (MQ=255) aaCGTGCAGGCTTCGAAGGTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTc < 2:435687/90‑1 (MQ=255) aaCGTGCAGGCTTCGAAGGTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTc < 1:186314/90‑1 (MQ=255) aaCGTGCAGGCTTCGAAGGTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTc < 1:252413/90‑1 (MQ=255) cGTGCAGGCTTCTAAGGTTGAGTCGTACACCCGACTCACTACGGTCGCGTATTTCCAATCTAAACCCCTGAAAGGCCGAACATCGGTCTg < 1:432464/90‑1 (MQ=255) cGTGCAGGCTTCGAAGGTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTg > 1:334741/1‑90 (MQ=255) cAGGCTTCGAAGGTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCa < 1:380031/90‑1 (MQ=255) aGGCTTCGAAGGTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCaa > 1:194795/1‑90 (MQ=255) cTTCGAAGGTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAActc > 1:38609/1‑90 (MQ=255) cTTCGAAGGTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAActc < 1:701380/90‑1 (MQ=255) cGAAGGTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAActctct > 2:327799/1‑90 (MQ=255) | CAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCT > NZ_CP009273/4172726‑4172896 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |