Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP009273822,9550TG64.3% 2.4 / 10.7 14T495P (ACC→CCC) ybhFATP‑binding cassette domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base T (0/5);  new base G (9/0);  total (9/5)
Fisher's exact test for biased strand distribution p-value = 5.00e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.61e-03
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

ATCCATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAAC  >  NZ_CP009273/822875‑823020
                                                                                |                                                                 
aTCCATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGAggggggt                                                          >  1:293863/1‑88 (MQ=255)
                          ccGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGGGGGGGGCAACCCCGGAAGGCGGGTCGTCGGGaa                                >  1:24980/1‑90 (MQ=255)
                              gACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGGGGGGGGGAACGCCGGGAGGCGGGTTGTCGCGAAAAAg                            >  2:484614/1‑90 (MQ=255)
                                     ttCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTcc                     <  2:420030/90‑1 (MQ=255)
                                      tctcTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGAGGGGGGCAACGCCGGAAGTCGGTTCGTCGAGAAAAAGAAAGTCCg                    >  1:32159/1‑90 (MQ=255)
                                         ctACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGtt                 <  1:484614/90‑1 (MQ=255)
                                              aTGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGGGGGGGGGAACCCCGGAAGGCGGGTCGTCGGGAAAAAGAAAGTTCGGGTCATGc            >  1:394235/1‑90 (MQ=255)
                                              aTGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGGGGGGGGCAACGCCGGGAGGCGGGTCGTCGAGAAAAAGAAAGTCCGGGTCATGc            >  2:363551/1‑90 (MQ=255)
                                              aTGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGAGGGGGGCAACGCCCGAAAGCGGGTGGTCGAGAAAAAAAAAATCCGGGTCATGc            >  1:217934/1‑90 (MQ=255)
                                                   gTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAg       <  1:352406/90‑1 (MQ=255)
                                                   gTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAg       <  2:368259/90‑1 (MQ=255)
                                                   gTTGATGTGCAGCCAAAATTCACGGCGGGGGAGGGGGGCAACGCCGGAAGGCGGGTCGTCGAGAAACAGAATGTCCGGGTCATGCATCAg       >  1:158466/1‑90 (MQ=255)
                                                        tgtgCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAAc  <  2:217934/90‑1 (MQ=255)
                                                        tgtgCAGCCAAAATTCACGGCGGGGGGGGGGGGCAACGCCGGAAGGCGGGTTGTCGAGAAAAAAAAAGTCCGGGTCCTGCCTCAGCGAAc  >  2:193000/1‑90 (MQ=255)
                                                                                |                                                                 
ATCCATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAAC  >  NZ_CP009273/822875‑823020

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑