Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP009273822,9630TG61.5% ‑0.3 / 11.9 13D492A (GAC→GCC) ybhFATP‑binding cassette domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base T (0/5);  new base G (8/0);  total (8/5)
Fisher's exact test for biased strand distribution p-value = 7.77e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.99e-03
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

CCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAACAGGCCAGCGCCAGCCGCTGTTTAAAACCTAAT  >  NZ_CP009273/822901‑823052
                                                              |                                                                                         
ccGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGGGGGGGGCAACCCCGGAAGGCGGGTCGTCGGGaa                                                                >  1:24980/1‑90 (MQ=255)
    gACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGGGGGGGGGAACGCCGGGAGGCGGGTTGTCGCGAAAAAg                                                            >  2:484614/1‑90 (MQ=255)
           ttCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTcc                                                     <  2:420030/90‑1 (MQ=255)
            tctcTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGAGGGGGGCAACGCCGGAAGTCGGTTCGTCGAGAAAAAGAAAGTCCg                                                    >  1:32159/1‑90 (MQ=255)
               ctACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGtt                                                 <  1:484614/90‑1 (MQ=255)
                    aTGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGGGGGGGGGAACCCCGGAAGGCGGGTCGTCGGGAAAAAGAAAGTTCGGGTCATGc                                            >  1:394235/1‑90 (MQ=255)
                    aTGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGGGGGGGGCAACGCCGGGAGGCGGGTCGTCGAGAAAAAGAAAGTCCGGGTCATGc                                            >  2:363551/1‑90 (MQ=255)
                    aTGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGAGGGGGGCAACGCCCGAAAGCGGGTGGTCGAGAAAAAAAAAATCCGGGTCATGc                                            >  1:217934/1‑90 (MQ=255)
                         gTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAg                                       <  1:352406/90‑1 (MQ=255)
                         gTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAg                                       <  2:368259/90‑1 (MQ=255)
                         gTTGATGTGCAGCCAAAATTCACGGCGGGGGAGGGGGGCAACGCCGGAAGGCGGGTCGTCGAGAAACAGAATGTCCGGGTCATGCATCAg                                       >  1:158466/1‑90 (MQ=255)
                              tgtgCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAAc                                  <  2:217934/90‑1 (MQ=255)
                              tgtgCAGCCAAAATTCACGGCGGGGGGGGGGGGCAACGCCGGAAGGCGGGTTGTCGAGAAAAAAAAAGTCCGGGTCCTGCCTCAGCGAAc                                  >  2:193000/1‑90 (MQ=255)
                                                              tCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAACAGGCCAGCGCCAGCCGCTGTTTAAAACCTAAt  >  1:4074/1‑90 (MQ=255)
                                                              |                                                                                         
CCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAACAGGCCAGCGCCAGCCGCTGTTTAAAACCTAAT  >  NZ_CP009273/822901‑823052

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑