| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,068,184 | 0 | T | G | 53.8% | 12.0 / 16.9 | 13 | T46P (ACT→CCT) | rutB | peroxyureidoacrylate/ureidoacrylate amidohydrolase RutB |
| Reads supporting (aligned to +/- strand): ref base T (1/5); new base G (7/0); total (8/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.66e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.56e-03 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GCCATTTTGAAACCAGATGATCAGCATCCCTGCCGCTCGCGCTGCGGTCACGGCGGTTTGAATGTTGGCAATGACCGGGCGAGTGGTTGAGACATCAAACCCGGCGAGATCTAAGTAGCCGCCTGGCGTGGCATAAGCGTTTTGCATATCCACCACGAT > NZ_CP009273/1068101‑1068259 | gCCATTTTGAAACCAGATGATCAGCATCCCTGCCGCTCGCGCTGCGGTCACGGCGGTTTGAATGTTGGCAATGACCGGGCGAGGGGGTga > 1:21076/1‑90 (MQ=255) gCATCCCTGCCGCTCGCGCTGCGGTCACGGCGGTTTTGATGTTGGGAAAAACCCGGGGAGGGGTTGGGAAATCAAAACCCGGGAGAGCTa > 1:507729/1‑90 (MQ=255) gCATCCCTGCCGCTCGCGCTGCGGTCACGGCGGTTTGAATGTTGGCAAAGACCGGGCGGGGGGGTGGGACATCAAACCCCGCGAGATCTa > 2:419566/1‑90 (MQ=255) aTCCCTGCCGCTCGCGCTGCGGTCACGGCGGTTTGGATGTTGGCAATGACCCGGCGGGGGGGTGGGACATCAAACCCGGGGGGGTCTAAg > 2:229911/1‑90 (MQ=255) tCCCTGCCGCTCGCGCTGCGGTCACGGCGGTTTGAATGTTGGCAATGACCGGGCGAGTGGTTGAGACATCAAACCCGGCGAGATCTAAGt < 1:434208/90‑1 (MQ=255) ccTGCCGCTCGCGCTGCGGTCACGGCGGTTTGAATGTTGGCAAAGACCGGGCGGGGGGGTGGGACATCAAACCCGGCGAGATCTAAGTAg > 2:434208/1‑90 (MQ=255) gCTCGCGCTGCGGTCACGGCGGTTTGGATGTTGGGAATGACCGGGGGAGGGGTTGAGACATCAAAACCCGCGAGGTCTAAGTAgacgccc > 2:363053/1‑89 (MQ=255) gCTCGCGCTGCGGTCACGGCGGTTTGAATGTTGGCAATGACCGGGCGAGGGGGTGGGACATCAAACCCGGCGAGATCTAAGTAgccgccc > 1:243623/1‑89 (MQ=255) ggTCACGGCGGTTTGAATGTTGGCAATGACCGGGCGAGTGGTTGAGACATCAAACCCGGCGAGATCTAAGTAGCCGCCTGGCGTGGCATa < 1:495755/90‑1 (MQ=255) aaTGTTGGCAATGACCGGGCGAGTGGTTGAGACATCAAACCCGGCGAGATCTAAGTAGCCGCCTGGCGTGGCATAAGCGTTTTGCATATc < 1:226709/90‑1 (MQ=255) aaTGTTGGCAATGACCGGGCGAGTGGTTGAGACATCAAACCCGGCGAGATCTAAGTAGCCGCCTGGCGTGGCATAAGCGTTTTGCATATc < 1:419566/90‑1 (MQ=255) tGGCAATGACCGGGCGAGTGGTTGAGACATCAAACCCGGCGAGATCTAAGTAGCCGCCTGGCGTGGCATAAGCGTTTTGCATATccacca < 2:243623/90‑1 (MQ=255) aaTGACCGGGCGAGTGGTTGAGACATCAAACCCGGCGAGATCTAAGTAGCCGCCTGGCGTGGCATAAGCGTTTTGCATATCCACCACGAt > 1:362323/1‑90 (MQ=255) | GCCATTTTGAAACCAGATGATCAGCATCCCTGCCGCTCGCGCTGCGGTCACGGCGGTTTGAATGTTGGCAATGACCGGGCGAGTGGTTGAGACATCAAACCCGGCGAGATCTAAGTAGCCGCCTGGCGTGGCATAAGCGTTTTGCATATCCACCACGAT > NZ_CP009273/1068101‑1068259 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |