| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,634,341 | 0 | C | T | 56.2% | ‑3.3 / 13.3 | 16 | F40F (TTC→TTT) | dtpB | dipeptide/tripeptide permease DtpB |
| Reads supporting (aligned to +/- strand): ref base C (2/5); new base T (9/0); total (11/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.81e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.64e-02 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTTTTCTTCGTTAAACAGCTTGGATTCTCGCAAGAGCAGGCTTTTGTCACTTTTGGTGCTTTTGCTGCGCTGGTCTATGGCCTC > NZ_CP009273/3634252‑3634419 | cagcaACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTTttc < 1:285792/90‑1 (MQ=255)cagcaACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTTttc < 1:42431/90‑1 (MQ=255) gcaACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTtttttt > 1:466578/1‑90 (MQ=255) aGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGGTTTTTTCTTTTAAAAACTTTGGTTTTCGCCAGAGCAGGCGTTTTTCACtt > 2:140259/1‑90 (MQ=255) gCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTTTTTTTTGTTAAAAAGCTTTGGTTTTCGCAAAAGCAGGGGTTTTTTACttt > 1:502561/1‑90 (MQ=255) cGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTTTTCTTCGTTTAACAGCTTGGATTTTCGCAAGAGCAGGCTTTTTTCACcttt > 1:170258/1‑90 (MQ=255) cGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGGTTTTTTTGTTTAAAAACTTTGGTTTTCCCCAAAGAAGGCGTTTTTTACtttt > 2:275650/1‑90 (MQ=255) ggCTACTACGGCGTGCAGGGCGTACTGGCGGTTTTCTTCGTTAAACAGCTTGGATTCTCGCAAGAGCAGGCTTTTGTCACTTTTGGTGCt < 1:183334/90‑1 (MQ=255) actacGGCGTGCAGGGCGTACTGGCGGGTTTTTTTGTTTAAAAGCTTTGGTTTTCCCCAGAGCCGGGTTTTTTTTCTTTTTGGGGttttt > 2:128189/1‑89 (MQ=255) ctacGGCGTGCAGGGCGTACTGGCGGTTTTCTTCGTTAAACAGCTTGGATTCTCGCAAGAGCAGGCTTTTGTCACTTTTGGTGCTTTtgc < 2:502561/90‑1 (MQ=255) gCGTGCAGGGCGTACTGGCGGGTTTTTTTCTTTAACAGCTTGGGTTTTCCCAAAAGCAGGGTTTTTTTCCTTTTTGGGCTTTTTCTgcgc > 2:67835/1‑90 (MQ=255) cGTGCAGGGCGTACTGGCGGGTTTTTTCGTTAAACAACTTGGATTCTCGCCAGAGCAGGCTTTTGTCACTTTTTGTGCTTTTTCTGCGCt > 1:460090/1‑90 (MQ=255) ggCGTACTGGCGGTTTTCTTCGTTAAACAGCTTGGATTCTCGCAAGAGCAGGCTTTTGTCACTTTTGGTGCTTTTGCTGCGCTGGTCTAt < 1:408179/90‑1 (MQ=255) gCGTACTGGCGGTTTTTTTCGTTTAACAGCTTGGATTTTCGCAAGAGCAGGCTTTTTTCACTTTTTGTGCTTTTTCTTCGCTGGGCTATg > 2:12455/1‑90 (MQ=255) tACTGGCGGTTTTTTTCGTTAAACAGCTTGGATTCTCGCAAGAGCAGGCTTTTTTTACTTTTTGTGCTTTTTCTTCGCTGGTTTTTGGcc > 2:121381/1‑90 (MQ=255) tACTGGCGGTTTTTTTCGTTAAACAGCTTGGATTCTCGCAAGAGCAGGCTTTTTTTACTTTTTGGGCTTTTTCTGCGCTGGGCTATGGcc > 2:142188/1‑90 (MQ=255) tACTGGCGGTTTTCTTCGTTAAACAGCTTGGATTCTCGCAAGAGCAGGCTTTTTTCACTTTTGGGGCTTTTTCTGCGCTGGTCTATGGcc > 1:260093/1‑90 (MQ=255) tACTGGCGGTTTTCTTCGTTAAACAGCTTGGATTCTCGCAAGAGCAGGCTTTTGTCACTTTTGGTGCTTTTGCTGCGCTGGTCTATGGcc < 2:466578/90‑1 (MQ=255) cTGGCGGTTTTCTTCGTTAAACAGCTTGGATTCTCGCAAGAGCAGGCTTTTGTCACTTTTGGTGCTTTTGCTGCGCTGGTCTATGGCCTc < 1:477179/90‑1 (MQ=255) | CAGCAACCTCGCCCATTTTTCATGATCTTTTTTGTCGAGTTATGGGAGCGATTCGGCTACTACGGCGTGCAGGGCGTACTGGCGGTTTTCTTCGTTAAACAGCTTGGATTCTCGCAAGAGCAGGCTTTTGTCACTTTTGGTGCTTTTGCTGCGCTGGTCTATGGCCTC > NZ_CP009273/3634252‑3634419 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |