Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP009273360,7590TG54.5% 3.6 / 11.4 11T329P (ACC→CCC) lacIDNA‑binding transcriptional repressor LacI
Reads supporting (aligned to +/- strand):  ref base T (1/4);  new base G (6/0);  total (7/4)
Fisher's exact test for biased strand distribution p-value = 1.52e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.28e-03
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

AACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCACCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCT  >  NZ_CP009273/360685‑360846
                                                                          |                                                                                       
aaCCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGGTTGCGGATTGGGCACCAGGGGGGGtttttttttttc                                                                          >  2:544349/1‑87 (MQ=255)
          gCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTTGGCACCAGGGGGGGTTTTTTTTTCACCACTGAGACg                                                                >  1:446379/1‑90 (MQ=255)
                           aTCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGGACCAGGGGGGGTTTTTTTTTTACCCGTGAGACGGGCAACAGCTGATTTcc                                               >  1:262628/1‑90 (MQ=255)
                           aTCGGCCAACGCGCGGGGAGAGGCGGTTTGCGGATTTGGCACCCGGGGGGGTTTTTTTTTCCCCAGGGGGGCGGGCAACAGCTGATTGcc                                               >  2:286927/1‑90 (MQ=255)
                              ggCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGGACCAGGGGGGGTTTTTTTTTTCCCAGGGAGACGGGCAACAGCTGATTGCCCtt                                            >  1:373399/1‑90 (MQ=255)
                                 cAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCACCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCAc                                         <  2:212560/90‑1 (MQ=255)
                                      cgcgGGGAGAGGCGGTTTGCGTATTGGGCACCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCt                                    <  1:349418/90‑1 (MQ=255)
                                               agGCGGTTTTCGTATTTGGCACCAGGGGGGTTTTTTTTTTTACCAGTGAGACGGGCAACAACGGATTGCCCTTTACCCCCTGGCCCTgag                           >  1:505886/1‑90 (MQ=255)
                                                    gTTTGCGTATTGGGCACCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGt                      <  1:439134/90‑1 (MQ=255)
                                                        gCGTATTGGGCACCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGc                                 >  1:171416/1‑75 (MQ=255)
                                                        gCGTATTGGGCACCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGc                                 <  2:171416/75‑1 (MQ=255)
                                                                        ggtggtTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCt  <  1:424644/90‑1 (MQ=255)
                                                                          |                                                                                       
AACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCACCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCT  >  NZ_CP009273/360685‑360846

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑