| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 585,390 | 0 | T | G | 54.5% | 0.7 / 12.9 | 11 | T341P (ACC→CCC) | nfrA | bacteriophage adsorption protein NfrA |
| Reads supporting (aligned to +/- strand): ref base T (0/5); new base G (6/0); total (6/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.16e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.26e-02 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CCAGACGCAGAGCTTCAGCCTTGTTACGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGCGGGGAGGGTGGCGAGCAGTTTTTGCGCTGCGTCGTACTGACCTTCTTTTAACAGCACCGGTAGCGTCGCGCCAACAACATACTGGCGGTTGTCGG > NZ_CP009273/585309‑585476 | ccAGACGCAGAGCTTCAGCCTTGTTACGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGCGGGGAGGGTGGCGAGCa < 1:477210/90‑1 (MQ=255) agCTTCAGCCTTGTTACGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATCGGCGGGGAGGGTGGCGAGCAGTTTTTgcgc < 2:8163/90‑1 (MQ=255) tCAGCCTTGTTACGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGCGGGGGGGGGGGCGGGCAGGTTTTTTGCTGcg > 1:13086/1‑90 (MQ=255) cTTGTTACGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGCGGGGGGGGGGGGGGGCAGGTTTTTCGCTGcgtcgga > 1:108991/1‑88 (MQ=255) tGTTACGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGCGGGGGGGGGGGGGGGGAGGTTTTGCGCTGCGGCGTCCt > 2:437727/1‑90 (MQ=255) aCGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGCGGGGAGGGTGGCGAGCAGTTTTTGCGCTGCGTCGTACTGAcc < 1:44493/90‑1 (MQ=255) tGACAGCATAACGCTCCTCAAGCATTTCATTGGCGGGGAGGGTGGCGAGCAGTTTTTGCGCTGCGTCGTACTGACCTTCTTTTAACAGCa < 1:237226/90‑1 (MQ=255) aTAACGCTCCTCAAGCATTTCATTGGCGGGGGGGGGGGGGGGCAGGTTTTTCGCTGCGTCGTACTGACCTTCTTTTTAAAACACCGGGAg > 2:237226/1‑90 (MQ=255) ttCATTGGCGGGGGGGGGGGGGGGCAGTTTTTTCGCTGCGTCGTACTGACCTTCTTTTTACAACACCCGGAGCGGCGCGCCCACAACATa > 1:273569/1‑90 (MQ=255) gCGGGGGGGGGGGCGAGCAGTTTTTTCGCTGCCGCGTACTGACCTTCTTTTTTTAACACCCGGGGCGGCGCGCCCACAAAATACTGgggg > 2:636465/1‑90 (MQ=255) gggTGGCGAGCAGTTTTTGCGCTGCGTCGTACTGACCTTCTTTTAACAGCACCGGTAGCGTCGCGCCAACAACATACTGGCGGTTGTCgg < 2:273569/90‑1 (MQ=255) | CCAGACGCAGAGCTTCAGCCTTGTTACGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGCGGGGAGGGTGGCGAGCAGTTTTTGCGCTGCGTCGTACTGACCTTCTTTTAACAGCACCGGTAGCGTCGCGCCAACAACATACTGGCGGTTGTCGG > NZ_CP009273/585309‑585476 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |