| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,336,778 | 0 | T | A | 63.6% | ‑0.4 / 22.1 | 22 | M291L (ATG→TTG) | yfaL | AIDA‑I family autotransporter adhesin YfaL/EhaC |
| Reads supporting (aligned to +/- strand): ref base T (8/0); new base A (0/14); total (8/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.13e-06 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.79e-05 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GTCAACAGCTCCGTCATTCTCTGTATTGCCAATAACCAGCGTTTTTCCGTCGG‑CAATATCAAAGGTAACTTCACTTAAGCCGAGATACATAAAGCCACCCGCCGCAGAGGAAGGACCATCTCCATAGCCTGCTGCGCTATTGTTCTCATCGACTAACACGCCTC > NZ_CP009273/2336689‑2336852 | gTCAACAGCTCCGTCATTCTCTGTATTGCCAATAACCAGCGTTTTTCCGTCGG‑CAATATCAAAGGTAACTTCACTTAAGCCGAGATACAt > 2:419929/1‑90 (MQ=255) cAACAGCTCCGTCATTCTCTGTATTGCCAATAACCAGCGTTTTTCCGTCGG‑CAATATCAAAGGTAACTTCACTTAAGCCGAGATACATaa > 2:456318/1‑90 (MQ=255) cAACAGCTCCGTCATTCTCTGTATTGCCAATAACCAGCGTTTTTCCGTCGG‑CAATATCAAAGGTAACTTCACTTAAGCCGAGATACATaa > 1:574489/1‑90 (MQ=255) gCTCCGTCATTCTCTGTATTGCCAATAACCAGCGTTTTTCCGTCGG‑CAATATCAAAGGTAACTTCACTTAAGCCGAGATACATAAAGCCa > 1:230322/1‑90 (MQ=255) ccGTCTTTCTCTGTATTCCCAAAAACCGGTTTTTTCCCGCCGG‑AAATATCAAAGGTACCTCCATTAAAGCCGAGATAAAAAAACcccccc < 2:460842/90‑1 (MQ=255) ccGTCATTCTCTGTATTGCCAATAACCAGCGTTTTTCCGTCGG‑CAATATCAAAGGTAACTTCACTTAAGCCGAGATACATAAAGCCAccc > 1:524801/1‑90 (MQ=255) gTCATTCTCTGTATTGCCAATAACCAGCGTTTTTCCGTCGG‑CAATATCAAAGGTAACTTCACTTAAGCCGAGATACATAAAGCCACccgc > 1:472378/1‑90 (MQ=255) gTATTGCCAATAACCAGCGTTTTTCCGTCGG‑CAATATCAAAGGTAACTTCACTTAAGCCGAGATACATAAAGCCACCCGCCGCAGaggaa > 2:205571/1‑90 (MQ=255) gTATTGCCAATAACCAGCGTTTTTCCGTCGG‑CAATATCAAAGGTAACTTCACTTAAGCCGAGATACATAAAGCCACCCGCCGCAGaggaa > 1:194714/1‑90 (MQ=255) ttGCAAATACCCAGCTTTTTCCCGCCGG‑AAATAAAAAAGGTAACTTCACTAAAGCCGAAATAAAAAAACCCCCCCGCCGCAGaggaagga < 2:194714/90‑1 (MQ=255) caaaTACCCAGCTTTTTCCCGTCGG‑CAATATCAAAGGTAACTTCACTTAAGCCGAGATACAAAAAGCCCCCCGCCGCAGAGGAAGGACCa < 1:451434/88‑1 (MQ=255) caaaTACCCAGCTTTTTCCCGCCGG‑AAATACAAAAGGAACCTTCCCTTAAGCCGAAATACAAAAAGCCCCCCGCCGCAGAGGAAGGACCa < 2:11133/88‑1 (MQ=255) cAATAACCGGCTTTTTCCCGGCGG‑AAATATAAAAGGAAACTTCCCTAAAGCCGAAATACAAAAACCCCCCCGCCGCAGAGGAAGGACCAt < 1:303928/90‑1 (MQ=255) aaaTAACCAGCTTTTTTCCGTCGG‑CAATATCAAAGGTAACTTCACTTAAGCCGAGATACAAAAAGCCCCCCGCCGCAGAGGAAGGACCAt < 1:511090/89‑1 (MQ=255) aaaTAACCAGCGTTTTCCCGTCGG‑AAAAATAAAAGGTAACTCCACTAAAGCCGAGAAACAAAAAGCCACCCGCCGCAGAGGAAGGACCAt < 2:602635/89‑1 (MQ=255) aaaaaCCCGGGTTTTTTCGTTGGACAATAAAAAAGTAAACTTCATTAAAGCGGAAATACAAAAACCCCCCCGCCGCAGAGGAAGGACCAt < 2:414020/87‑1 (MQ=255) aaTAACCAGCGTTTTTCCGTCGG‑CAATATCAAAGGTAACTTCACTTAAGCCGAGATACATAAAGCCACCCGCCGCAGAGGAAGGACCAtc > 2:556299/1‑90 (MQ=255) ttttCCGGGGG‑CAATAAAAAAGGTACTTTCACTAAAGCAGAAAAAAAAAAACCCCCCCGCCGCAGAGGAAGGACCATCTCCATAGCctgc < 2:591635/90‑1 (MQ=255) tCCGTCGG‑CAATATCAAAGGTAACTTCACTTAAGCCGAAATACAAAAAGCCCCCCGCCGCAGAGGAAGGACCATCTCCATAGCCtgctgc < 1:205571/90‑1 (MQ=255) aaaaGGTAACTTCCCTAAAGCCGAGATACAAAAAGCCCCCCGCCGCAGAGGAAGGACCATCTCCATAGCCTGCTGCGCTATTGTTCTCAt < 1:456318/89‑1 (MQ=255) gTAACTTCACTAAAGCCGAAATAAAAAAACCCCCCCGCCGCAGAGGAAGGACCATCTCCATAGCCTGCTGCGCTATTGTTCTCATCGACt < 1:603867/90‑1 (MQ=255) ttAAGCCGAAATAAAAAAAGCCCCCCGCCGCAGAGGAAGGACCATCTCCATAGCCTGCTGCGCTATTGTTCTCATCGACTAACACGCCTc < 1:419929/90‑1 (MQ=255) taaaGCCGAAATACAAAAACCCCCCCGCCGCAGAGGAAGGACCATCTCCATAGCCTGCTGCGCTATTGTTCTCATCGACTAACACGCCTc < 2:230322/88‑1 (MQ=255) | GTCAACAGCTCCGTCATTCTCTGTATTGCCAATAACCAGCGTTTTTCCGTCGG‑CAATATCAAAGGTAACTTCACTTAAGCCGAGATACATAAAGCCACCCGCCGCAGAGGAAGGACCATCTCCATAGCCTGCTGCGCTATTGTTCTCATCGACTAACACGCCTC > NZ_CP009273/2336689‑2336852 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |