| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,667,520 | 0 | A | C | 53.3% | 8.1 / 16.5 | 15 | G176G (GGT→GGG) | yphB | aldose 1‑epimerase |
| Reads supporting (aligned to +/- strand): ref base A (4/3); new base C (0/8); total (4/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.56e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.22e-02 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TCCGGCAAAACCATTGTTCACCCACTGGCGCGGCAACGGCGCGGGCTGGTTAAAATCCAGTTCCTGCGGTAGCTGCTCGCAAAACTCACCCGCCAGCCACTGCTCCCGCTCCAGCCAGTAACCGCTCGCCTGTGCCTGAATCCGCGTTTGCGGCGACAACGGAAAATAAGG > NZ_CP009273/2667433‑2667603 | tCCGGCAAAACCATTGTTCACCCACTGGCGCGGCAACGGCGCGGGCTGGTTAAAATCCAGTTCCTGCGGTAGCTGCTCGCAAAACTCAcc < 1:344766/90‑1 (MQ=255) cGGCAAAACCATTGTTCACCCACTGGCGCGGCAACGGCGCGGGCTGGTTAAAATCCAGTTCCTGCGGTAGCTGCTCGCAAAACTCACCCg > 1:580161/1‑90 (MQ=255) cAAACCCATTGTTCCCCCACTGGCGCGGCAACGGCGCGGGCTGGTTAAAATCCAGTTCCTGCGGTAGCTGCTCGCAAAACTCACCCgcca < 2:414074/90‑1 (MQ=255) aCCATTTTCCCCCCACGGGCGCGGCAACGGCGGGGGCTGGTAAAAACCCATTCCCTGGGGTAGCTGCTCGCAAACCTCCCCCgccagcca < 2:220065/90‑1 (MQ=255) tCACCCACTGGCGCGGCAACGGCGCGGGCTGGTTAAAATCCAGTTCCTGCGGTAGCTGCTCGCAAAACTCACCCGCCAGCCACTGCTccc > 2:494279/1‑90 (MQ=255) cccgggCGCGGCAACGGCGGGGGCGGTTAAAAACCCATTCCCGGGGGTAGCTGCTCGAAAACCCCCCCCGCCAGCCACTGCTCCCGCTcc < 2:616225/86‑1 (MQ=255) gcgcGGCAACGGCGCGGGCTGGTTAAAATCCAGTTCCTGCGGTAGCTGCTCGCAAAACTCACCCGCCAGCCACTGCTCCCGCTccagcca > 1:64222/1‑90 (MQ=255) gcgGCAACGGCGCGGGCTGGTTAAAATCCAGTTCCTGCGGTAGCTGCTCGCAAAACTCACCCGCCAGCCACTGCTCCCGCTCCAGCCAGt > 1:251723/1‑90 (MQ=255) cgGCAACGGCGCGGGCTGGTTAAAACCCAGTTCCTGCGGTAGCTGCTCGCAAAACTCACCCGCCAGCCACTGCTCCCGCTCCAGCCAGTa < 1:592011/90‑1 (MQ=255) ggCGGGGGCTGGTTAAAATCCAGTTCCTGGGGTAGCTGCTCGAAAAACTCCCCCGCCAGCCACTGCTCCCGCTCCAGCCAGTAACCGCTc < 1:571149/90‑1 (MQ=255) gcgcGGGCTGGTAAAAACCCAGTCCCTGCGGTAGCTGCTCGAAAACCTCCCCCGCCAGCCACTGCTCCCGCTCCAGCCAGTAACCGCTCg < 2:64222/90‑1 (MQ=255) aacccAGTTCCGGCGGTAGCGGCCCGCAAACCTCCCCCGCCAGCCACTGCTCCCGCTCCAGCCAGTAACCGCTCGCCTGTGCCTGAATcc < 2:241157/87‑1 (MQ=255) aacccAGTCCCTGCGGTAGCTGCTCGCAAACCCCCCCCGCCAGCCACTGCTCCCGCTCCAGCCAGTAACCGCTCGCCTGTGCCTGAATcc < 2:251723/87‑1 (MQ=255) gTAGCTGCTCGCAAACCTCCCCCGCCAGCCACTGCTCCCGCTCCAGCCAGTAACCGCTCGCCTGTGCCTGAATCCGCGTTTGCGGCGACa < 1:426527/90‑1 (MQ=255) aaccTCCCCCGCCAGCCACTGCTCCCGCTCCAGCCAGTAACCGCTCGCCTGTGCCTGAATCCGCGTTTGCGGCGACAACGGAAAATAAgg < 1:469251/87‑1 (MQ=255) | TCCGGCAAAACCATTGTTCACCCACTGGCGCGGCAACGGCGCGGGCTGGTTAAAATCCAGTTCCTGCGGTAGCTGCTCGCAAAACTCACCCGCCAGCCACTGCTCCCGCTCCAGCCAGTAACCGCTCGCCTGTGCCTGAATCCGCGTTTGCGGCGACAACGGAAAATAAGG > NZ_CP009273/2667433‑2667603 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |