| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 4,227,178 | 0 | T | C | 60.0% | 7.6 / 16.0 | 15 | D118D (GAT→GAC) | yjbG | capsule biosynthesis GfcC family protein |
| Reads supporting (aligned to +/- strand): ref base T (4/2); new base C (0/9); total (4/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.10e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.47e-04 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TCCAGCGCCGACGATGCCGCTGCGATTAACGCCTTACGCCAGCAAATTCAGGCGTTGAAAGTGACGGGCAGGCAAAAAATCAATCTTGATCCCGATATCGTCCGCGTTGCCGAACGCGGTAACCCGCCGTTGCAGGGCAACTACACGCTGTGGGTCGGACCACCGCCGTCCAC > NZ_CP009273/4227089‑4227261 | tCCAGCGCCGACGATGCCGCTGCGATTAACGCCTTACGCCAGCAAATTCAGGCGTTGAAAGTGACGGGCAGGCAAAAAATCAATCTTGAt < 1:32819/90‑1 (MQ=255) cAGCGCCGACGATGCCGCTGCGATTAACGCCTTACGCCAGCAAATTCAGGCGTTGAAAGTGACGGGCAGGCAAAAAATCAATCTTGATcc < 2:142938/90‑1 (MQ=255) gacgaTGCCGCTGCGATTAACGCCTTACGCCAGCAAATTCAGGCGTTGAAAGTGACGGGCAGGCAAAAAATCAATCTTGATCCCGATATc > 1:42112/1‑90 (MQ=255) cGCTGCGATTAACGCCTTACGCCAGCAAATTCAGGCGTTGAAAGTGACGGGCAGGCAAAAAATCAATCTTGATCCCGATATCGTCCGCGt < 2:621357/90‑1 (MQ=255) ttACGCCAGCAAATTCAGGCGTTGAAAGTGACGGGCAGGCAAAAAATCAATCTTGATCCCGATATCGTCCGCGTTGCCGAACGCGGTAAc > 2:103000/1‑90 (MQ=255) gCAAATTCAGGCGTTGAAAGTGACGGGCAGGCAAAAAATCAATCTTGATCCCGATATCGTCCGCGTTGCCGAACGCGGTAACCCGCCGtt > 1:392412/1‑90 (MQ=255) aaaTTCAGGCGTTGAAAGTGACGGGCAGGAAAAAAATCAATCTTGACCCCGATATCGCCCGCGTTGCCGAACGCGGTACCCCGCCGTTGc < 1:546717/90‑1 (MQ=255) gAAAGTGACGGGCAGGCAAAAAATCAATCTTGACCCCGATATCGCCCGCGTTGCCGAACGCGGTAACCCGCCGTTGCAGGGCAACTacac < 2:95156/90‑1 (MQ=255) aaaaGTGAGGGGCAGGAAAAAAATCAATCTTGACCCCGATATCGCCCGCGTTGCCGACCGCGGTAACCCGCCGTTGCAGGGCAACTacac < 2:59122/89‑1 (MQ=255) gTGACGGGCAGAAAAAAAACCACTCTTGACCCCGATATCGCCCGCGTCGCCGACCGCGGAAACCCGCCGTTGCAGGGCAACTACACGCtg < 2:424995/90‑1 (MQ=255) tGACGGGCAGGAAAAAAATCAATCTTGACCCCGATATCGTCCGCGTTGCCGAACGCGGTAACCCGCCGTTGCAGGGCAACTACACGCtgt < 1:307267/90‑1 (MQ=255) gACGGGCAGGCAAAAAATCAATCTTGATCCCGATATCGTCCGCGTTGCCGAACGCGGTAACCCGCCGTTGCAGGGCAACTACACGCtgtg > 2:245626/1‑90 (MQ=255) ggcaggaaAAAAATAAATTTTGACCCCGATTTCGTCCGGGTTGCCGAACGGGGTACCCCGCCGTTGCAGGGCAACTACACGCTGTGGGTc < 1:418234/90‑1 (MQ=255) aaaTCAATCTTGACCCCGATATCGTCCGCGTTGCCGAACGCGGTAACCCGCCGTTGCAGGGCAACTACACGCTGTGGGTCGGACCAccgc < 1:162959/90‑1 (MQ=255) acttttGACCCCGATACCGCCCGCGTTGCCGACCGCGGTAACCCGCCGTTGCAGGGCAACTACACGCTGTGGGTCGGACCACCGCCGTcc < 2:298030/86‑1 (MQ=255) tcTTGACCCCGATACCGCCCGCGTTGCCGAACGCGGTACCCCGCCGTTGCAGGGCAACTACACGCTGTGGGTCGGACCACCGCCGTCCAc < 2:42112/90‑1 (MQ=255) | TCCAGCGCCGACGATGCCGCTGCGATTAACGCCTTACGCCAGCAAATTCAGGCGTTGAAAGTGACGGGCAGGCAAAAAATCAATCTTGATCCCGATATCGTCCGCGTTGCCGAACGCGGTAACCCGCCGTTGCAGGGCAACTACACGCTGTGGGTCGGACCACCGCCGTCCAC > NZ_CP009273/4227089‑4227261 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |