| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 4,492,581 | 0 | A | C | 54.2% | 7.7 / 28.7 | 24 | L229R (CTG→CGG) | insG | IS4‑like element IS4 family transposase |
| Reads supporting (aligned to +/- strand): ref base A (6/5); new base C (13/0); total (19/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.09e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.43e-04 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CCCAGTTTTCTGATCTCTTCATATTGCGCTCCCTTTCTGAGAGGTATCATCCAGTGGCGGTGTTCTCCCGCCAGGCTCCAGGCATTTAACAGTCCCAGTGAGTAAT‑AACCTTTATCCATTAACGTCAGAGTGTTATCGCCGGTTTGTTCTATAAGTTGC > NZ_CP009273/4492509‑4492667 | cccAGTTTTCTGATCTCTTCATATTGCGCTCCCTTTCTGAGAGGTATCATCCAGTGGCGGTGTTCTCCCGCCAGGCTCCAGGCATTTAAc < 1:352348/90‑1 (MQ=255) cAGTTTTCTGATCTCTTCATATTGCGCTCCCTTTCTGAGAGGTATCATCCAGTGGCGGTGTTCTCCCGCCAGGCTCCAGGCATTTAACAg < 1:587614/90‑1 (MQ=255) gTTTTCTGATCTCTTCATATTGCGCTCCCTTTCTGAGAGGTATCATCCAGTGGCGGTGTTTTCCCCCCCCGCTCCCGGCATTTTACAGTc > 2:599240/1‑90 (MQ=255) gTTTTCTGATCTCTTCATATTGCGCTCCCTTTCTGAGAGGTATCATCCAGTGGCGGTGTTCTCCCCCCCGGCCCCCGGCCTTTTACAATc > 1:458287/1‑90 (MQ=255) ctctTCATATTGCGCTCCCTTTCTGAGAGGTATCATCCAGTGGCGGTGTTTTCCCCCCCGGCTCCAGGCATTTAACAGTCCCAGTGAGta > 2:166877/1‑90 (MQ=255) ctctTCATATTGCGCTCCCTTTCTGAGAGGTATCATCCAGTGGCGGTGTTCTCCCGCCAGGCTCCAGGCATTTAACAGTCCCAGTg < 2:569170/86‑1 (MQ=255) ctctTCATATTGCGCTCCCTTTCTGAGAGGTATCATCCAGTGGCGGTGTTCTCCCGCCAGGCTCCAGGCATTTAACAGTCCCAGTg > 1:569170/1‑86 (MQ=255) ctTCATATTGCGCTCCCTTTCTGAGAGGTATCATCCAGTGGCGGTGTTCTCCCCCCCGGCTCCCGGCCTTTAACAATCCCCGTGAGgaat > 1:279604/1‑90 (MQ=255) tatTGCGCTCCCTTTCTGAGAGGTATCATCCAGTGGCGGTGTTTTCCCCCCAGGCTCCCGGCATTTAAAAGTCCCAGTGAGTAAT‑AACCt > 1:506131/1‑90 (MQ=255) ttGCGCTCCCTTTCTGAGAGGTATCATCCAGTGGCGGTGTTCTCCCGCCa < 2:9479/50‑1 (MQ=255) ttGCGCTCCCTTTCTGAGAGGTATCATCCAGTGGCGGTGTTCTCCCGCCa > 1:9479/1‑50 (MQ=255) cgcTCCCTTTCTGAGAGGTATCATCCAGTGGCGGTGTTTTCCCCCCCGGCTCCCGGCATTTTAAAGTCCCCGTGGGTAAT‑AAACtttttt > 1:455475/1‑87 (MQ=255) cgcTCCCTTTCTGAGAGGTATCATCCAGTGGCGGTGTTCTCCCCCCCGGCTCCAGGCATTTAACAGTCCCAGTGAGTAATAAACCttttc > 1:536253/1‑88 (MQ=255) cccTTTCTGAGAGGTATCATCCAGTGGCGGTGTTTTTCCCCCCGGCTCCCGGCATTTTACAGTCCCCGGGGGGAAT‑AACCTTTTTCCAtt > 2:578647/1‑90 (MQ=255) gagGTATCATCCAGTGGCGGTGTTCTCCCCCCCGGCTCCCGGCATTTAACAGTCCCCGTGGGGAAT‑AACCTTTTTCCCTTAACGTCagag > 2:63619/1‑90 (MQ=255) gagGTATCATCCAGTGGCGGTGTTCTCCCCCCCGGCTCCAGGCATTTAACAGTCCCAGTGAGTAAT‑AACCTTTTTCCATTAACGTCagag > 2:163646/1‑90 (MQ=255) ggTATCATCCAGTGGCGGGGTTCTCCCGCCCGGCTCCCGGCATTTAACAATCCCCGGGGGGAAT‑AACCTTTTTCCCTTAACGTCagaggg > 2:295973/1‑88 (MQ=255) tATCATCCAGTGGCGGTGGTCTCCCCCCCGGCTCCAGGCCTTTAACAGTCCCAGTGAGGAAT‑AACCTTTATCCATTAACGTCAGAGGGtt > 1:619976/1‑90 (MQ=255) atcatcCAGTGGCGGTGTTCTCCCGCCCGGCTCCAGGCATTTAACAGTCCCAGTGGGTAAA‑AACCTTTATCCATTAACGTCAGAGGGTTa > 1:536786/1‑90 (MQ=255) aGTGGCGGTGTTCTCCCCCCAGGCTCCAGGCATTTAACAGTCCCAGTGAGTAAT‑AACCTTTATCCATTAACGTCAGAGGGTTATCGCCgg > 1:510382/1‑90 (MQ=255) gTGGCGGTGTTCTCCCGCCAGGCTCCAGGCATTTAACAGTCCCAGTGAGTAAT‑AACCTTTATCCATTAACGTCAGAGTGTTATCGc < 2:397924/86‑1 (MQ=255) gTGGCGGTGTTCTCCCGCCAGGCTCCAGGCATTTAACAGTCCCAGTGAGTAAT‑AACCTTTATCCATTAACGTCAGAGTGTTATCGc > 1:397924/1‑86 (MQ=255) ggTGTTCTCCCGCCAGGCTCCAGGCATTTAACAGTCCCAGTGAGTAAT‑AACCTTTATCCATTAACGTCAGAGGGTTATCGCCGGtttttt > 1:69877/1‑90 (MQ=255) ggTGTTCTCCCCCCCGGCTCCAGGCATTTAACAGTCCCAGGGAGGAAT‑AACCTTTTTCCATTAACGTCAGAGGGTTATCGCCGGgttgtt > 2:405546/1‑90 (MQ=255) cGCCAGGCTCCAGGCATTTAACAGTCCCAGTGAGTAAT‑AACCTTTATCCATTAACGTCAGAGTGTTATCGCCGGTTTGTTCTATAAGTTg > 1:140358/1‑90 (MQ=255) gCCAGGCTCCAGGCATTTAACAGTCCCAGTGAGTAAT‑AACCTTTATCCATTAACGTCAGAGTGTTATCGCCGGTTTGTTCTATAAGTTGc < 2:536253/90‑1 (MQ=255) | CCCAGTTTTCTGATCTCTTCATATTGCGCTCCCTTTCTGAGAGGTATCATCCAGTGGCGGTGTTCTCCCGCCAGGCTCCAGGCATTTAACAGTCCCAGTGAGTAAT‑AACCTTTATCCATTAACGTCAGAGTGTTATCGCCGGTTTGTTCTATAAGTTGC > NZ_CP009273/4492509‑4492667 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |