| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 17,050 | 0 | A | C | 53.8% | 10.0 / 17.0 | 13 | intergenic (‑147/‑439) | mokC/nhaA | type I toxin‑antitoxin system toxin MokC/Na+/H+ antiporter NhaA |
| Reads supporting (aligned to +/- strand): ref base A (6/0); new base C (0/7); total (6/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.83e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.56e-03 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GGCCTCGGGTTGATGGTAAAATATCACTCGGGGCTTTTCTCTATCTGCCG‑TTCAGCTAATGCCTGAGACAGACAGCCTCAAGCACCCGCCGCTATTATATCGCTCTCTTTAACCCATTTTGTTTTATCGATTCTAATCCTGAAGACGCCTC > NZ_CP009273/16967‑17117 | ggCCTGGGGTTTAGGATAAAATCTCCCGCGGGTTTTTTCTCTTCCTCCCG‑TTCAGCTAATGCCGGAGAAACACACCCCCAAGCCCccgcc < 2:95256/90‑1 (MQ=255)ggCCTGGGGTTGATGGAAAAATATCCCTCGGGTTTTTTCTCTTTCTCCCG‑TTCAGCTAATGCCTGAGACAGACAGCCTCAAGCCCccgcc < 2:427473/90‑1 (MQ=255)ggCCTCGGGTTGATGGTAAAATATCACTCGGGGCTTTTCTCTATCTGCCG‑TTCAGCTAATGCCTGAGACAGACAGCCTCAAGCACccgcc > 1:450306/1‑90 (MQ=255)ggCCTCGGGTTGATGGTAAAATATCACTCGGGGCTTTTCTCTATCTGCCG‑TTCAGCTAATGCCTGAGACAGACAGCCTCAAGCACccgcc > 1:513037/1‑90 (MQ=255)ggCCTCGGGTTGATGGTAAAATATCACTCGGGGCTTTTCTCTATCTGCCG‑TTCAGCTAATGCCTGAGACAGACAGCCTCAAGCACccgcc > 2:125667/1‑90 (MQ=255)ggCCTCGGGTTGATGGTAAAATATCACTCGGGGCTTTTCTCTATCTGCCG‑TTCAGCTAATGCCTGAGACAGACAGCCTCAAGCACccgcc > 2:566485/1‑90 (MQ=255) ccTCGGGTTGATGGTAAAATATCACTCGGGGCTTTTCTCTATCTGCCG‑TTCAGCTAATGCCTGAGACAGACAGCCTCAAGCACCcgccgc > 1:242795/1‑90 (MQ=255) ccTCGGGTTGATGGTAAAATATCACTCGGGGCTTTTCTCTATCTGCCG‑TTCAGCTAATGCCTGAGACAGACAGCCTCAAGCACCcgccgc > 1:427473/1‑90 (MQ=255) gggtttGATGAAAAAATACCCCGGGGGGTTTTTCTCTCTCGCCCT‑TTCAGCAAATGCCTGAGACAGACCCCCCCAACCCCCCGCCGCtat < 2:513037/86‑1 (MQ=255) aaaaaaaaACACGCGGGCTTTTTCTCTTTCTGCCT‑TTCGGTTAACGCCGGAGAAAGACACCCACAAGCCCCCGCCGCTATTATATCGctc < 2:125115/88‑1 (MQ=255) gggCTTTTCTTCTCCCGCCGTTTCAGCAAACGCCTAAGAAAACCACCCCAAACCCCCCGCCGCTATTATATCGCTCTCTTTAACCCAttt < 1:566485/90‑1 (MQ=255) gCCT‑TTCACCAAATGCCTGAGACAGACACCCCCAACCCCCCGCCGCTATTATATCGCTCTCTTTAACCCATTTTGTTTTATCGATTCTaa < 1:533026/90‑1 (MQ=255) ccTGAGACAGCCACCCTAAACCCCCCGCCGCTATTATATCGCTCTCTTTAACCCATTTTGTTTTATCGATTCTAATCCTGAAGACGCCTc < 2:153500/90‑1 (MQ=255) | GGCCTCGGGTTGATGGTAAAATATCACTCGGGGCTTTTCTCTATCTGCCG‑TTCAGCTAATGCCTGAGACAGACAGCCTCAAGCACCCGCCGCTATTATATCGCTCTCTTTAACCCATTTTGTTTTATCGATTCTAATCCTGAAGACGCCTC > NZ_CP009273/16967‑17117 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |